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Showing: 121 - 140 of 293 results

  1. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...solution. Since this technique is intensive and requires an electron microscope, it is not routinely performed...a few ways:  Absolute quantification by qPCR requires generating a standard curve of known concentration...

  2. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... tracrRNA and crRNA to cleave target DNA (Cpf1 requires only a crRNA for target recognition and cleavage... PFS. This is in stark contrast to Cas9, which requires that each DNA target have high sequence identity...

  3. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...look like (the 3rd sequencing trace). While it requires a little extra wet lab work, this is still a great...edits and look for off-target changes. This method requires a set of control cells to compare the sequencing...

  6. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...for how sensitive you can make a reaction. NLuc requires 100-fold less protein to produce the same amount...detecting traditional luciferases’ dim light emissions requires long acquisition times that may be impractical...

  8. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...writing can take weeks to a couple of months and requires that all of the experiments have been completed...thoughtful engaging talk takes significant effort. It requires evaluation of all of the data and any recent relevant...

  9. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...world, so a process or project or mission that requires a broad knowledge base is going to necessarily...the type of team. Knowing what roles your team requires to function is certainly necessary, but equally...

  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...'t require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic) Digenome...doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to time since ...

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...neuronal tracing compounds and this is an area that requires further study. Traditional neuronal tracing methods...for neuronal tracing and network identification requires both antero- and retrograde labelling, synaptic...

  14. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...on or off the expression of shRNA. This system requires less work than using established methods and minimizes...thorough understanding of complex biological systems requires both a catalog of molecular players and contextualized...
Showing: 121 - 140 of 293 results