...flowers that change color throughout the day) and doing this with very little money. So when did inspiration...biology PhD’s. I was facing the prospect of having to do a third postdoc in order to rebuild my resume, so...we spent a lot of time talking about what we could do to make ourselves marketable to the wider world and...altogether. We had a lot of ideas about things we could do for work, and they weren’t restricted to science ...some point, our ideas got pretty good. We started doing extracurricular research on some of them to see ...along the lines of “I hate GMOs, but what you are doing makes sense. I can support that.” That was interesting...building this company, and that’s what we want it to do. It’s exhilarating to have that sort of freedom. ...

...organization and see how I can help. Q: What do you enjoy doing outside of work? BN: I love to travel and...interesting. It’s very varied. There’s IP work to do, general corporate work, and negotiating contracts...bring to Addgene -- that comfort with saying, “if we do this, then what?” I hope to bring that business experience...

... unrelated to do (many Addgenies enjoy rock climbing, for example) and let your brain do some thinking...had to think creatively to solve a problem. But how do you intentionally engage in a creative problem-solving...during. (Boyes, 2019) If you’ve taken the time to do this and are still struggling with idea generation...on your problem-solving resources. What would you do if you had all the funding in the world? What if ...if you could hire more people? What would you do with unlimited time? Another strategy for your brainstorm...participants feel comfortable sharing their ideas? And how do we give people the opportunity to speak out and prevent...If all else fails, one of the best things you can do for yourself when you’re stuck on a problem is to...

...Here are a few easy and fast things you can start doing right away to reduce your consumption in the lab...on all the latest news and encourage your peers to do the same. Long-term lab conservation goals Lastly...motivated and to feel accomplished. In addition to doing some of the quick things above, try joining or starting... -80 °C freezer to -70 °C and teach other labs to do the same. -70 °C is cold enough to keep some common...

...together 10-gene systems, what do you want to make? So, tell us readers, what do you want to make?   References..., and the future of the bioeconomy. Addgene: What do these BIOFAB and BIL gates kits – the first to be...Typically when people use plasmids, they are trying to do one thing at a time. What the BioBricks Foundation...every primary metabolite in a cell. The only way to do that at scale right now is to grow up a bunch of ...displays for control and visualization. Addgene: What do you envision for the future as this programming language... You can now forward engineer a 10-gene system to do something. We know from our testing of these parts...

...and @HelloPhD "Role" call. #BLACKandSTEM what do you do? — Stephani Page, PhD 👩🏾‍🔬💜 (@ThePurplePage... I'm my lab's resident #scicomm expert. What tips do you have for using social media as a scientist? pic.twitter.com...of Twitter and LinkedIn probably had something to do with it. Five resources to help you get started ...people for about a week and then unfollow those who do not capture your interest during that week.” You ...be useful, but it’s also one more thing on your to do list. This video shows quick ways social media can...

...ve been able to watch the growth since then. What do you think is the most important resource that Addgene...and other necessary information that’s required to do research. Paratore: What I’ve been most impressed...distributing them and spending more time on research. What do you hope Addgene will accomplish in the near future...progresses in 2019 and beyond. In your perspective, what do you think are important considerations for the future...Master the fundamentals. Before you can go off and do great things in a particular area or profession, ... fundamentals.   What are your favorite things to do out of the office? Katz: I have a puppy and so I ...

...figure out where they bind to the genome. We’ve been doing that for years using this assay called ChIP-seq,... In adapting the CRISPR/Cas system, what we are doing is adding in a second plasmid to target an epitope...quickly? Myers: We were hoping to figure out how to do this for many hundreds of transcription factors. ...antibody problem. We thought it was the right thing to do to put these out there. It’s a simple solution. It...from FLAG to GFP or a different epitope. Addgene: Do you have any additional tips, tricks, or words of... social pressure on many other labs that may be doing something similar. There may be other variants of...

...they do it.    Finding Conflict in Science  In all science communication, what you are doing is telling...they’re firmly part of the story to the scientists doing the work.  And while these pieces aren't always ...of my story” and that’s a very valid critique! But do you remember earlier when I said that even when we...t that sense of curiosity and wonder why you’re doing and sharing science in the first place?   References...

...that we are doing just that. Do you like the direction the blog has taken over the past year? Do you think... 7. Career Coaching for Scientists: Why and Where Do I Find One? If you need help making a transition...Addgenie Joanne Kamens explains what career coaches do and offers up some career coach suggestions. 8. ...

...to the principle that you can't motivate people to do things, you can only demotivate them. The primary...less a really good one). While getting your PhD or doing a postdoc few science trainees will have opportunities...hard and most people have some learning they need to do before they can be good at it. Like any other skill...freedom and diversity built into their jobs Chance to do/learn new things, diversity of responsibilities, ...if opportunities for learning and personal growth do not exist or if they feel underutilized in their ...

...Cancer. 2003 Jan;3(1):11-22. PubMed PMID: 12509763 . Do you have suggestions for other plasmids that should...

...specific repetitive DNA sequences in human chromosomes Do you have suggestions for other plasmids that should...

...more versions of this plasmid. Root Return to Top Do you have suggestions for other plasmids that should...

...customer place an Addgene order using a PO number? Why do I have to order directly from your website? Many ...sent to the contact email addresses provided. How do I place an order using a PO number? Placing an order... PO, learn more about how to create a quote. How do I pay the invoice? After your Addgene order ships...this article, check out our Help Center article How do I pay by purchase order (PO) number? You can find...

... news and updates. Scientists must do science to be happy. What do we have to change to ensure that all...all scientists can have successful careers doing science in some form? There is a lot of talk about the... behind and some just don't have the resources to do enough. There are still university research departments...

...researchers in the field. Dos and don’ts when publishing a scientific manuscript Dos: Make sure your paper...editor or reviewer over their decision. If you do object, do so in a respectful and professional manner. ...need to include in your manuscript. What information do I need to include in my paper? More and more journals...

...you in building a grant career. Do you have a passion for the arts? Do you find satisfaction in helping...Chat, and then our grant training and mentoring. How do I become a grant professional? Employment opportunities...healthcare facilities and with private businesses. How do you get the expertise needed to enter the grants ...helping your local food shelf raise money? Do you have experience with research grants? Are you interested...find work and target your professional development. Do you want to work in a particular area, e.g. education... childhood development, or community development? Do you want to focus on a part of the grant development...

...Wood of Duke University Medical Center. “While we do not yet have a comprehensive understanding of how... become resistant, an emerging theme is that they do so by activating signaling pathways controlling properties...contexts, so he assembled a plasmid kit designed to do exactly that. As described in the journal Science... world who are interested to use our libraries to do things - sometimes drug screens and sometimes totally...

...are trying to achieve: Do you want to permanently knock out or knock-in a gene? Do you want to activate ...Are you trying to create a single point mutation? Do you want to create a fusion with a reporter protein...appropriate expression system and delivery method: Do you need stable integration or is transient expression...sufficient? Which cell types will you be editing? Do you want to deliver the components as DNA, or would...surprised at the number of options available - how do you choose between them? One of the first steps is...