...Hill University of Illinois Chicago                     Boston Children's Hospital                           ...fighting the pandemic could be even more impactful by reaching more scientists. Many members of the scientific...industry-based scientific community. Conversations with children playing in the background became a normal occurrence...Institute                                       Chinese Academy of Medical Sciences University of Texas...plasmids quickly and Addgene’s quality control and archiving system allowed us to handle and distribute such...

... and were only available as green probes. The Patriarchi Lab aimed to overcome these limitations by developing...researchers looking to study norepinephrine, the Patriarchi lab continues to work on further improvements...nLightG. Many thanks to Mike Waring and Tommaso Patriarchi for their assistance with this blog post. References...and resources References Feng, J., Zhang, C., Lischinsky, J. E., Jing, M., Zhou, J., Wang, H., Zhang, ...Benke, D., Weber, B., Bohacek, J., Reiner, A., … Patriarchi, T. (2023). Sensitive multicolor indicators for...Mirdita, M., Schütze, K., Moriwaki, Y., Heo, L., Ovchinnikov, S., & Steinegger, M. (2022). ColabFold: Making...

...experiment. Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in... cell. These so-called recombinase-based state machines could potentially be used to report upon and/or...post to learn more about recombinase-based state machines and how they function. Adapting Toehold Switches... describes R-bodies, “force generating protein machines that can extend and retract over many microns ...you can better design your donor DNA template to achieve more efficient genome editing - all influenced ...

...relationships, so that you can reap rewards in the form of reaching career development goals. Check out Joanne's Reddit... adjust expectations, and record milestones in reaching goals. The more time and thought you put into ...setting goals, the more progress you will make in reaching them. Of course, you won’t reach all the goals...happens. Mentors bring more than their knowledge and teaching to help a Mentee reach goals. One of the most ...sometimes it is only the knowledge that they will be watching out for me that gets me out of bed and into my...being a Mentor: Learn other perspectives Develop coaching skills Encourage your creative thinking Enhance...

...specificity far greater than the one that can be achieved using pharmacological or electrophysiological ...., 2016; Hudry and Vandenberghe, 2019). How to achieve strong opsin expression in specific neuronal cell...expression in vivo (Schnütgen et al., 2003). This is achieved through site-specific recombinases such as Cre...expression in specific neuronal cells? This can be achieved using Cre-dependent viruses, such as the optogenetic... functional topography of the neural circuit architecture of the brain regions associated with motivated...love for educational science led her to pursue a teaching career, and she now is a very happy scientific... and approaches for optogenetics." Biological psychiatry 71.12 (2012): 1033-1038. PubMed PMID: 22480664...

...traditional cell culture, enriching your active RNAi subpopulation can be easily achieved through fluorescence-activated...resulting in processing by the endogenous RNAi machinery. Alternatively, endogenous miRNA precursors can...initial state of the dsRNA upon activating the RNAi machinery (long dsRNA, typically hairpin RNA, or artificial...gene target to silencing of the reporter. This is achieved at the level of the DNA construct, where your ...itself is indicative of active gene silencing. We achieved this by creating constructs that directly fused...Polytechnic Institute where he investigated how plants achieve polarized cell growth in the lab of Luis Vidali...

...provide them on your smart phone. 1. Lab Archives by LabArchives The single, most important part of lab ... life. These apps prevent you from constantly searching the interwebs for lab resources, and instead conveniently...lab life is the lab notebook. Lab Archives is a multipurpose application that allows you to share your data...notebook from your smartphone to your computer. Lab Archives will help both you and your lab stay organized...from 2 years ago. The free subscription to Lab Archives allots for 25 MB of space per user, but, if you...

...than KillerRed, with KillerRed reaching greater than 75% photobleaching after just 1 minute of exposure...produce ROS - targeted cell ablation via ROS is achieved through localized expression of the FAP. A new...portion of the complex and is derived from the malachite green (MG) dye, but has been modified with the...longer exposure times (5 minutes vs 10 seconds) to achieve the same level of killing as Mg-2I-dL5**. Mg-2I-dL5...exposure. MG-2I-dL5** experienced minor bleaching after longer exposure times. Additionally, KillerRed requires...

...gRNAs are released by endogenous tRNA processing machineries, specifically, RNaseZ and RNaseP. Although the... for gene editing. They named this new array architecture the DAP array, short for “drive-and-process....length of 5’ leader sequences of a given tRNA to achieve the highest efficiencies of multiplex editing at...also engineered a DAP array for prime editing, achieving up to 3-loci MPE with similar high efficiencies...payloads. Different from existing multiplex array architectures, including previous tRNA-gRNA arrays, that rely...Comparison of previous representative dual-gRNA architectures with DAP array   Qichen Yuan is a PhD...

...immensely. Paratore: I have two children active in sports, so I enjoy watching their games.  If I’m not at....Paratore: I’ve certainly had a front row seat watching the international growth through my work on the...graduated with highest honors from the University of Michigan, Ann Arbor and obtained her J.D. from Boston University...CISA), and certified health insurance executive (CHIE). He is a member of both the Internal Institute ...Food Bank and participates locally in Read To A Child's "Lunchtime Reading Program" in Cambridge, MA. ...

...What is an Antibody animation!  Isotype switching Isotype switching occurs when a B cell changes which heavy...other isotype heavy chain constant domains. This switching helps B cells adapt during the course of an immune...the effector function of the antibody. Isotype switching is caused by a specialized type of DNA recombination...IgG are deleted.     Figure 2: Isotype switching requires DNA recombination of the antibody gene...constant domain gene. This process is called isotype switching and results in the B cell expressing an antibody...

...-based policy and legislation hit home hard in Michigan, where programs like the Great Lakes Restoration...planet. And as we rallied on the University of Michigan campus speakers thoughtfully reminded us to use...a postdoctoral researcher in the University of Michigan Department of Astronomy. She studies how planets...tirelessly to get the publications, grant funding, and teaching experience that it takes to complete your resume...march ended that I loved - there were booths for children to learn little things about science. We need ...to be the perfect opportunity to cement in my children's heads the importance of science and positively... that most who participated will consider that marching in and of itself will be enough. IT WON’T! Promoting...

...transcription machinery.   Well, I’ve got some good news: complete DNA demethylation can be achieved by dCas9...can be achieved by fluorescence-activated cell sorting, and stable gRNA expression can be achieved using... activity. They interact with transcriptional machinery to modify local gene expression, confounding any...doi.org/10.1016/j.neuron.2013.08.032.  Morita S, Noguchi H, Horii T et al. (2016) Targeted DNA demethylation...

...for in vivo delivery of advanced genome editing machinery such as CRISPR-Cas. Figure 1: Schematic...transduced to target cells. Within target cells, Cas9 machinery is transiently expressed and binds to target DNA...rates of targeted mutagenesis similar to those achieved by isogenic AdVs encoding TALENs targeting the...). If sufficient gene transfer and knock-in is achieved, producer cells could produce corrective amounts...potential platform that could be realized via simple switching of the relevant gRNAs and donor DNA Adenoviral...Han Y-C, Ogrodowski P, Crippa A, Rekhtman N, de Stanchina E, Lowe SW, Ventura A (2014) In vivo engineering...

... that many researchers were having difficulty achieving indel formation (gene disruption) with this new...most of the respondents have been struggling to achieve genome editing with NgAgo, this does not mean the...successful indel generation and epitope knockin was achieved by a few respondents suggesting that NgAgo can...most of the researchers surveyed here could not achieve indels with the NgAgo system indicates that it ...which might be rapidly degraded by the cellular machinery, leaving a small amount of active NgAgo/ssDNA ... cell. As proposed in the original article, to achieve greater efficacy, researchers will have to carry...

...domain. Feng Zhang's group utilized the bi-lobed architecture of Cas9 to engineer a series of "split" cas9...must not act on any of the other bits in order to achieve accurate and reliable multi-bit memory. To this...cassette, you can specifically express your new chimeric protein in a variety of model systems. The MultiSite...but a translated mRNA will be red. This system achieves great specificity and resolution by using 6-12...mitochondrial subpopulations within the same cell; by photoswitching PSmOrange and photoactivating PAGFP in separate...screened for speed and voltage sensitivity. This hierarchical screen led to the generation of two enhanced... CMV-LUC2CP/intron/ARE). The Luc-I intron is a chimeric β-globin/immunoglobulin intron that has been commonly...

... plasmids to utilize diverse host replication machinery. For instance, the ori typically contains iterons...However, if plasmids have their own replication machinery such as Rep or other initiator proteins, they ...vulgaris, Erwinia carotovora, E. chrysanthemi, Escherichia coli, Gluconacetobacter xylinus, Gluconobacter...E. herbicola, E. rubrifaciens, E. stewartii, Escherichia coli, Klebsiella spp., Legionella pneumophila...spp., Brucella spp., Caulobacter crescentus, Escherichia coli, Gluconacetobacter xylinus, Paracoccous ...integron promoter-based expression systems for Escherichia coli and Agrobacterium tumefaciens. 3 Biotech...

... passes through the machine’s lasers and gives off a signal read by the machine’s sensors. (Usually this...or unbound antibodies.) Each event read by the machine is plotted as a dot on an X,Y axis to generate ...variations in fluorescent signals, and how the machine reads/interprets the signals, the axis is instead...populations. This shape is called a ‘gate’; it tells the machine that everything inside the gate is one population...Doloff, J., Bose, S., Vegas, A., Ma, M., Sahay, G., Chiu, A., Bader, A., Langan, E., Siebert, S., Li, J.,...

...become popular. There are only so many methods and machines one team can learn so linking together expert ... a mailing list. This project gave me practice reaching out to other scientists, being clear about what...beyond your organization and/or scientific field. Reaching out to people who have little in common with you...starting a collaboration with a colleague. However, reaching outside your knowledge sphere allows you to connect...time to talk or to at least read my emails. Then pitching myself and the event was very difficult and somewhat...experience during my graduate studies in regard to reaching out to new people, checking in regularly, and ...

...scientists and non-scientists alike are marching for. I will be marching to advocate for science education ...is a Pro-Science and Pro-Evidence march. Who is marching and why? Who? All sorts of people are choosing...promises more cuts (3, 12, 13, 27). In order to achieve the breakthroughs, technologies, and treatments...exhaustive list and every marcher is not necessarily marching for every cause. The references provided also ...opinions or research on each topic. Resources for marching and advocacy If the march for science rallies ...