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Showing: 121 - 140 of 461 results

  1. Reflections and Looking Ahead

    Type
    Blog Post
    ...Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F., Bradbury, A. R. M., Cable, H., & Longworth, S. (2024...References Ayoubi, R., Ryan, J., Gonzalez Bolivar, S., Alende, C., Ruiz Moleon, V., Fotouhi, M., Alqazzaz...

  2. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T. Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi...FISH) or fluorescently labeled TALEs, CRISPR/Cas9’s ease of use and ability to label live cells make this...

  3. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...thanks to our guest blogger, Harshana S De Silva Feelixge. Harshana S De Silva Feelixge is a researcher whose...This post was contributed by guest blogger Harshana S De Silva Feelixge. Gene therapy technologies hold...science communication.     References 1. Gaspar, H. B., S. Howe, and A. J. Thrasher. "Gene therapy progress ...

  5. Genetic Code Expansion

    Type
    Collection
    ...209744 pAB228v TrpRS S. cerevisiae Bacterial Ahmed Badran 209745 pAB228v6 TrpRS S. cerevisiae Bacterial...)RS Methanogenic archaeon S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-...Badran 209746 pAMC070n1a12 ScTrpRS, Lum1PylRS, MmPylRS S. cerevisiae, Lum1, M. mazei Bacterial Ahmed Badran...

  6. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...10.3389/fimmu.2017.01589 Jin B, Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation...https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung S, et al (2021) Nanobodies from camelid mice and llamas...

  8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...Pike-Overzet, K., Chatters, S. J., de Ridder, D., Gilmour, K. C., Adams, S., Thornhill, S. I., Parsley, K. L.,...10.1038/gt.2009.135. PubMed PMID: 19847206 Desfarges, S., & Ciuffi, A. (2012). Viral Integration and Consequences...: 23792628 PubMed Central PMCID: PMC3773023 Howe, S. J., Mansour, M. R., Schwarzwaelder, K., Bartholomae...

  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...180. PubMed PMID: 11815306. 4. Nicole Kresge, R. D. S. and R. L. H. “The Discovery of Avidin by Esmond E... (2004). PubMed PMID: 20353954. 5. Kay, B., Thai, S., & Volgina, V. “High-Throughput Biotinylation of ...PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules...Central PMCID: PMC4461904. 9. Huang, Q., Mao, Z., Li, S., Hu, J., & Zhu, Y. “A non-radioactive method for ...PMC4439073. 12. Ludwig, L. B., Hughes, B. J., & Schwartz, S. A. “Biotinylated probes in the electrophoretic mobility...

  10. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire...technique to subdivide neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson ...Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature...Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development...

  11. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...plasmid deposit. Figure 2: Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information...plasmids.     Figure 3: If submitting plasmid(s) online (left), choose to submit as published or unpublished...with an asterisk are required. If submitting plasmid(s) using a spreadsheet (right), click the “Download ...following descriptions for your plasmid: Encodes insert(s) A plasmid with one or more genes, protein coding...if you’d like to distribute your bacterial strain(s) through Addgene. Description – please indicate the...Figure 7: Enter information about the gene/insert(s). Step 3: Cloning Information Please enter the name...

  12. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...10.1016/j.jconrel.2019.02.009 Wang D, Mou H, Li S, Li Y, Hough S, Tran K, Li J, Yin H, Anderson DG, Sontheimer...the plasmids at Addgene: pAdSh.PGK.Cas9 (expresses S. pyogenes Cas9 from the PGK promoter) and U6 promoter-driven...Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan CA, Rader DJ, Musunuru...

  13. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER...Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S, Graham BS, Callewaert N, Schepens B, Saelens X, McLellan...Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA (2020) Generation...

  15. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...produce the primary recombinant adenoviral stock (rAdV-S). HEK293 cells are transfected with the AdV plasmid...The resultant lysate is your primary low titer rAdV-S, and can be stored at -80C for later use or used immediately...very low titer. Amplification (1-2 weeks) - The rAdV-S is used to infect more HEK293 cells and produce more...8892868. PubMed Central PMCID: PMC190817. 5. Lee, Cody S., et al. "Adenovirus-mediated gene delivery: Potential...

  16. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...When complexed with a full-length gRNA, native Cas9's two catalytic domains, HNH and RuvC, cleave DNA strands... to dramatically alter levels of expression. Cas9’s ability to alternately serve both as a means of modifying...gRNAs An alternative approach is to modulate Cas9’s nuclease activity by modifying the gRNA with which...nucleotides of complementarity to a target site, Cas9’s DNA binding capability remains intact while its nucleolytic...

  17. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...multiple Cas9 variants including high fidelity Cas9’s. Further advancements have been made by optimizing...

  18. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...technology! Resources and References References Orguc, S., Sands, J., Sahasrabudhe, A., Anikeeva, P., & Chandrakasan...https://doi.org/10.1109/EMBC44109.2020.9175600 Park, S., Loke, G., Fink, Y., & Anikeeva, P. (2019). Flexible...C8CS00710A Sahasrabudhe, A., Rupprecht, L. E., Orguc, S., Khudiyev, T., Tanaka, T., Sands, J., Zhu, W., Tabet...

  19. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher... Scher, D. S. (1994). Isotype choice for chimeric antibodies affects binding properties. In Journal of...

  20. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...they think they are.  References Berns KI, Adler S (1972) Separation of two types of adeno-associated...Maynard LH, Smith O, Tilmans NP, Tham E, Hosseinzadeh S, Tan W, Leenay R, May AP, Paulk NK (2019) Fast-Seq... . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao R, Maurer...
Showing: 121 - 140 of 461 results