Skip to main content
Addgene
Showing: 121 - 140 of 413 results

  2. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

    Type
    Blog Post
    ...Konermann explained, was new knowledge about Cas9’s crystal structure. “I thought maybe we could use information...target DNA. Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki...activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena...

  3. Plasmids for Stem Cell Research

    Type
    Collection
    ...induced pluripotent stem cells. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. Capecchi Replicating...single polycistronic vector. Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):157-62. Jaenisch Lentivirus ... of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek Fibroblasts Cardiomyocytes...fibroblasts to dopaminergic neurons. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10343-8. Parmar Fibroblasts...tripotent neural precursor cells. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32. Wernig Fibroblasts ...

  4. Microbiology Resources

    Type
    Collection
    ...constructs for S. cerevisiae - Dueber Lab Yeast Prototrophy : Restoration of prototrophy in common S. cerevisiae...

  5. RNA Extraction Without A Kit

    Type
    Blog Post
    ... for at least 4 hours.   If your final RNA sample(s) are resuspended in water or TE buffer, store them...break down RNA upon cell lysis.  In the late 1970’s, Chirgwin and colleagues showed that a strong protein...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...

  7. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...compared head-to-head with the E. coli and BioID2 BirA*s in the context of RaPID for their ability to detect...proteins were then subtracted from Ramanathan et al.’s RaPID datasets. Applications of RaPID RaPID sounds..., Rao, D.S., Neela, P.H., Zarnegar, B.J., Mondal, S., Roth, J.G., Gai, H., Kovalski, J.R., Siprashvili...

  8. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular cloning system for standardized...transcribed together. They can express one or more gene(s), and typically begin with a promoter and end with...

  9. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... to dramatically alter levels of expression. Cas9’s ability to alternately serve both as a means of modifying...gRNAs An alternative approach is to modulate Cas9’s nuclease activity by modifying the gRNA with which...nucleotides of complementarity to a target site, Cas9’s DNA binding capability remains intact while its nucleolytic...

  10. To Each HIS Own

    Type
    Blog Post
    ...Natl Acad Sci U S A. 1991;88(20):8972-6. Moks T, Abrahrnsen L, Oesterlof B, Josephson S, Oestling M, Enfors...polyhistidine tag, alternative fusion tags were used, such as S. aureus’ Protein A (Moks 1987). These tags, however...

  11. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ....  Mahlandt, E. K., Palacios Martínez, S., Arts, J. J., Tol, S., van Buul, J. D., & Goedhart, J. (2023...single-component systems take advantage of BcLOV4’s light-induced binding of the plasma membrane. In previous...doi.org/10.1016/j.ceb.2015.08.005.  Tei, R., Bagde, S. R., Fromme, J. C., & Baskin, J. M. (2023). Activity-based...

  12. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...plant cells. Like other model systems, expression of S. pyogenes Cas9 or Cas9 variants (hereafter referred.... References Belhaj K, Chaparro-Garcia A, Kamoun S, Nekrasov V (2013) Plant genome editing made easy:...https://doi.org/10.1128/mmbr.67.1.16-37.2003 Khatodia S, Bhatotia K, Passricha N, Khurana SMP, Tuteja N (2016...7: . https://doi.org/10.3389/fpls.2016.00506 Kwak S-Y, Lew TTS, Sweeney CJ, Koman VB, Wong MH, Bohmert-Tatarev...

  16. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene(s) from Ensembl genome browser: https:/...index.html Design crRNA/ssODN: Using Ensembl Transcript ID(s) as an input query, pick crRNA (guide RNA sequence...Garcia-Diaz C, Blin C, Grant V, Bulstrode H, Gogolok S et al. 2017. Efficient CRISPR/Cas9-assisted gene targeting...PubMed PMID: 9928493. 4. Liang X, Potter J, Kumar S, Ravinder N, Chesnut JD. 2017. Enhanced CRISPR/Cas9...

  18. Qi Lab CRISPR Page

    Type
    Collection
    ...system, CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) pathway, as an RNA-guided...nuclease-deficient Cas9 (dCas9) protein and a customized s ingle g uide RNA (sgRNA) designed with a 20-basepair...

  19. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9), potential target sites are...10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J, Beaver CM, Younger... Ding Q, Collins R, Ragavendran A, Brand H, Erdin S, Cowan CA, Talkowski ME, Musunuru K (2014) Low Incidence...10.1016/j.stem.2014.04.020 Yang L, Guell M, Byrne S, Yang JL, De Los Angeles A, Mali P, Aach J, Kim-Kiselak...

  20. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...yeast inducible CRISPRi library - Ampl133 targets S. cerevisiae genes with 6-12 guides per gene where ...new anti-CRISPRs were identified that inhibit the S. aureus Cas9, a Cas protein that is smaller and easier...pre-synaptic targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui Zeng'...
Showing: 121 - 140 of 413 results