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Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Addgene Executive Director Chonnettia Jones reflects on highlights of 2024 and shares exciting plans for 2025.

This blog post explores how CRISPR/Cas9 can be used to visualize multiple intrachromosomal loci in vivo and map the 4D nucleome.

Learn how and why AAV vectors are being used in cell and gene therapies.

2024 is the Year of Camelids! To celebrate, we are taking a look at how these species (e.g. camels, llamas, alpacas) have impacted biological research.

SPARC is a tool that labels a fraction of a genetically-defined subset of cells. The result: easy visualization of individual, non-overlapping cells.

Find CRISPR plasmids to study the regulatory networks involved in retinal development.

This post describes the biology and use of integrase-deficient lentiviral vectors in comparison to standard lentiviral vectors.

Learn how biotin is used in a variety of molecular biology experiments and makes it easier for researchers to label and purify macromolecules of interest.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Use plasmids to develop synthetic nanobodies in a few weeks with a three step selection process.

Learn how to produce, purify, and titer adenoviral vectors for your research.

Researchers at the Wyss Institute for Biologically Inspired Engineering explain how you can use truncated gRNAs to regulate gene expression.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

A review of a new brain to gut neural fiber technology from the Anikeeva and Bohorquez labs.

Right variable region, but wrong constant? Chimeric antibodies may be the answer!

Viral genome sequencing is a high-throughput method for determining AAV transgene identity and confirming the serotype.

TOPO cloning is a restriction enzyme and ligase free cloning method. Learn how TOPO works in this post.