We narrowed to 285 results for: nif
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TypeBlog Post... Organizing a thoughtful engaging talk takes significant effort. It requires evaluation of all of the ...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...
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Sequencing Options for CRISPR Genotyping
TypeBlog Post... amplicon sequencing (Hendel et al. 2015), a significant difference as CRISPR experiments move closer ...networks in biological systems. NGS will also play significantly into the realm of clinical therapeutic development... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo kits are designed...j.xgen.2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific... -
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post... applications that involve ranges of pH, and significant lengths of time, the stability of fluorescence...biology, fluorescent labeling techniques have significantly enhanced our understanding of cellular mechanisms... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...enough. Nashville, TN, USA 36.1627°N 86.7816°W- Jennifer Stancill I am a research biologist by training...supporters is much larger and stronger that I expected. Jennifer Stancill, Ph.D., is currently a post-doctoral ... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...the immune mechanisms of the host. The cause, manifestation, diagnosis, recurrence, and treatment of cancer...differ in practically every facet starting from manifestation to their specific interactions with different... -
Antibody Validation for Flow Cytometry
TypeBlog Post...PFA-triton). The fix/perm solutions can make a significant difference in the efficacy of your antibody. ..., PhD, MRCP, from Only Good Antibodies, with significant input from Michael Biddle, PhD, also from Only... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog Post...C-terminal end of PEmax to produce PE7. PE7 significantly improves editing efficiencies compared to PEmax...Liu's lab! This post was originally written by Jennifer Tsang in October 2019 and updated by Emily P. ... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post... manuscript could only have been viewed as a significant improvement. As a result, the software piñata...those engaging in CRISPR design, but it is a significant hurdle made worse by an ever increasing marketplace... -
Technical Design of a Western Blot
TypeBlog Post...However, they have high cross-reactivity, vary significantly from lot-to-lot, and have to be re-validated...option to cast your own gel. Handcast gels are significantly cheaper and generate less waste. However, they... -
Protocol - How to Create a Bacterial Glycerol Stock
TypeProtocol...freezing (5-6 times). Make sure that you see one uniform solution, and there are no layers present. Be sure... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol...available. Unlike the plastic tray, this will not significantly reduce the UV, but will protect the UV box from... -
Protocol - How to Perform a Diagnostic Digest
TypeProtocol...or enzymes) that cuts within the insert, but significantly off center (ideally around 1/3 of the way from... -
Centrifugation
TypeProtocol...volume as your samples. If your samples are significantly higher density than water, then you may need... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog Post... Jennifer Tsang... -
Help Us Name Blugene’s New Virus Friend
TypeBlog Post... Jennifer Tsang... -
Protocol - Bacterial Transformation
TypeProtocol.... Pro-Tips Commercial competent cells range significantly in their transformation efficiency. The lowest... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog Post... Jennifer Tsang... -
It’s CRISPR Week at Addgene!
TypeBlog Post... Jennifer Tsang... -
Participate in the Pets of Addgene Scavenger Hunt
TypeBlog Post... Jennifer Tsang...