...Read this Catalyst Report “Making Mentoring Work” to learn about what makes a good mentoring program. ...guidance. Second, I will offer some advice on how to “make the ask” once you have found someone you admire ...professional presence, approach them for advice on what makes them that way. Is there a senior person whose career...Choose 1-2 tactics to try. One of my favorites is making the pledge to have lunch or coffee with someone...able to: Help identify the skills you will need to make a change Provide perspective on core values at another...mentoring program, consider tapping into it and helping make it work by being an educated mentee. Other opportunities...and time. Consider this option but be cautious and make sure to choose one that comes highly recommended...

...BioBrick parts to make more materials: to make more fuel, to make more chemicals, to make more medicine. ... of DNA, transcription of DNA to make RNA, translation of RNA to make proteins. That's central to everything...systems, what do you want to make? So, tell us readers, what do you want to make?   References: Jerome Bonnet...BioBricks Foundation is seeking to accomplish is to make the engineering of biology much easier. So you could...much fun and so incredibly useful that we wanted to make them free to use by everybody. (For more on these...really plugging into the "stuff sector" if you will, making chemicals and materials, and we anticipate plugging...imagination. The question is: what can you use biology to make? If you think about the BIOFAB collection and really...

...let’s chat about giving science talks and how to make them serve you well. The happy byproduct might just...results in the order they were performed does not make for a memorable presentation anywhere but in a lab...knowing your audience is that it can help you avoid making mistakes. I once made a joke about social science... story. Using stories is one of the best ways to make you and your presentations engaging and memorable...you are more likely to remember the point.   What makes a story? A story is a narrative connecting cause...all the time, you just need to figure out how to make it suspenseful. 4. Start preparing your talk early...them. Of course, if some of your work does relate, make sure to include that material, but don’t force it...

...off your work at a conference, you need to first make your poster. While a poster generally contains the...participants can identify you. You may also wish to make creative use of a footer in your poster. This is...sections, so you can include a lot more. Font size: Making your poster readable While we are talking about...This strategy helps your sections stand out, and makes the entire poster more navigable.  Choose your content...that it will display your data most prominently, making it the star of your presentation. Portrait. This...potentially more catchy, but it can be trickier to make figures fit nicely into circular shapes rather than...color you select for the background, you’ll want to make sure that the color of your font is compatible. ...

...volunteer to lead a charitable cause.  While I can’t make it “all better” for you, I can offer some tips and...that end, here are my personal 10 commandments for making it all work – most of the time. #1 Thou shalt find... so will your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead...management tools and tactics to plan your work and make this happen. Be realistic and: Your co-workers ...done your way. Be patient with yourself; you will make progress over time. #6 Thou shalt throw money at...and deliver your groceries. Do anything you can to make your life easier and to have more time to relax ...enthusiastically foster independence Your partner can make his or her own dinner if you are going away to a...

...community: Diversity makes the difference  Association for Psychological Science: Diversity Makes Better Science...formally, governments are the organizations that make and enforce the rules of the lands we live on. Rules...cannot declare that gravity change direction and make things fly up when we drop them. Scientific realities...to an earlier analogy, no amount of shouting will make an apple fall up. As scientist and politician Rush...will fall up instead of down when dropped will not make it happen. In a time when a top official can be ... the job of having evidence”, scientists want to make sure having evidence is the job of everyone in government...Immigration Pollution Healthcare Science education To make scientists relatable (17, 20). This is an effort...

... information makes your manuscript more valuable to the research community. It also makes it more likely...Materials and methods section of their manuscript to make resources easier to identify, find back, and share...enough information to replicate your experiments, making research more reproducible and your results more...reviewers and other researchers to access the data and make up their own minds on whether the data analysis ...reliability of science publications. Peer reviewing To make sure your manuscript is perceived as a reliable ...ts when publishing a scientific manuscript Dos: Make sure your paper fits the scope of the journal you...Guidelines of the journal you are submitting to and make sure your manuscript fits the house style and prerequisites...

...organizations have been working to provide tools that make it easier to share in-depth information about reagents...FPbase are working hard to enable researchers by making it easier to find information about research tools... more descriptive and interactive protocols that make it much easier to learn new experimental techniques...Electronic lab notebooks also play a role here by making it easier to retain searchable, thorough notes ...techniques, an important component of reproducibility is making sure that others can understand your analyses and...data analysis while other organizations like Dryad make it easier for researchers to share data thereby ...published data if you need to. Similarly, FigShare also makes it easier to share data through their curation of...

... to produce constructs and edit genomes so as to make plants, and agricultural crops in particular, resistant...general. We are funded by a charity and since we are making all the tools for plant research here and validating...like for people to use them. The MoClo Plant Kit makes it really easy to clone genes for expression in ... the time. Now there are many, so if you want to make a 10-gene construct, you don’t have to worry about...plants, including many things people already use. It makes the switch to Golden Gate Cloning easier because...pathogens. It’s also easy to express genes in, which makes it a good laboratory workhorse. Tips & tricks Addgene... that you need from the kit and getting started making your own constructs with Golden Gate MoClo. The...

...Did you see Blugene’s make-over and our new homepage? If you've visited Addgene’s homepage recently, ...messaging, as well as simplifying the page, and making it all look clean and easy to use.  Easily search...global navigation and menus over the past year, making it much easier to get around our site.  Community...navigation, we also wanted to emphasize the values that make Addgene unique: community, sharing, and fun. The...reagents and knowledge with one another, and Addgene make this process easy and fun every day.  With these...three values with each element. We also set out to make sure the page looked clean, professional, and modern...exploration throughout the site.  Blugene gets a make-over You may also have noticed another big update...

...receive such a call, make sure to get that person’s contact information and again, make a personal connection... the hundreds of others that did the same thing? Make an effort to meet people. Attend smaller talks within... CV or resume). If you receive a card in return, make sure to follow up with a professional “thank you...company. It does, however, offer you an opportunity to make a personal connection; the importance of which will...there, so that you can tailor your CV accordingly. Make it stand out with key words and by putting your ...’ll find a variety of listings. Post your CV and make it visible, which will sometimes result in a recruiter... a traditional training program, and you hope to make a career in academia, you are likely in good shape...

...NB) were generated in pMAK:  pMAK461(NBαRabitb-IgG-Fc, Addgene #140699) pMAK462 (NBαMouse/Rat-IgG1-Fc...#140700) pMAK463 (NBαMouse-IgK, Addgene #140701) pMAK464 (NBαEGFP, Addgene #140702) The pMAK461-64 plasmids...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...which alleviates the inclusion body problem and makes nanobody production in E.coli as simple as following...miniprep protocol!  First, the authors created the pMAK backbone by modifying pMAL-T-Avi-His/BirA, a vector...used for in vivo biotinylation via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by ...

... paper into an electronic environment and hence making it searchable and shareable, but some ELNs provide...community were to: (1) prevent vendor lock in by making it easy to get data out of the ELN; and (2) facilitate...re-used frequently in the group’s research – it makes no sense and is not practical to move all this data...references the record of research is incomplete, making it difficult or impossible to reproduce. As the...external data sources, they also need to continue to make use of other tools they use in research. This includes...sharing and reproducibility ecosystem protocols.io makes possible. Connectivity to open source data repositories...have been launched and are now a main vehicle for making research data available for public access, review...

...goals. These tools remind us of our daily tasks, make our days more effective, and our schedules more ...research. Electronic lab notebooks (ELNs) are here to make lab work much more reproducible, convenient and ...required every once in a while, so it is good to make sure you’ll be able to do it when needed. How many.... If these are important in your lab, you should make sure your ELN allows you to assign specific permissions...ownership should be yours (your institution’s), so make sure you don’t use ELN providers that take any ownership...set for every member of your team. Do you need to make sure your research can be patented? I.e. is automated...difficult to figure out all the ways a given ELN can make your lab life easier. If you can talk to an ELN ...

...information about AAV, read our AAV guide . Can I make a stable cell line with adenovirus? No, adenoviral...

... as well as the instructions on how to make your own, will make it easier for you to use genomic tagging...alleninstitute.org. A classic challenge in cell biology is making sure that what we observe through the microscope...and result in a ubiquitous background signal that makes it hard to visualize the precise location of the...cellular structures, we provide guidance on how to make your own gene-tagging constructs below. Read on ...process following the double strand break CRISPR/Cas9 makes at the target site. The whole segment—a GFP tag ...cells. As a precautionary measure, it’s also good to make a few single base pair mutations in crRNA target...and destroyed by CRISPR/Cas9 during transfection. Making the double strand break and introducing the tag...

...Coaching can be an excellent tactic for scientists making a career shift or who wish to improve their current... committed to. Finally, hiring a paid coach will make you take the experience more seriously and I have...good ones take this responsibility seriously. As I make my rounds speaking at research institutions and ... Sometimes only a few hours of coaching time can make an enormous difference so don’t assume that this... with. Talk to the coach about their “style” and make sure this is a style that will help you. A good ...good coach usually will be a clear communicator and make direct suggestions for improvement and change. ...launched an online career coaching program that might make her coaching more affordable for you. There is also...

...nice if you can afford them). I am talking about making sure the audience is interested, educated and talking...with room for fortuitous improvisation. You want to make it look effortless to the audience, but the organizers...moderator should have a well-planned approach to making the event interesting and engaging. Long before...funny, provocative or interesting stories that will make for a fun discussion. People are much better at ...interesting questions and topics in advance is key. Make sure all your panelists have the correct date, location...as it approaches. Room set-up and logistics To make a really lively discussion, I strongly suggest asking...colleagues to events for this purpose. If possible, make sure the panelists are prepared to stay around to...

... in the world knows it—to be the first person to make a discovery. I can’t tell anyone else what the “...cases, don’t enjoy, is worthwhile and can help us make decisions about what we want to do in the future...only demonstrates initiative and drive, but also makes for an experience more tailored to personal interests...professors about their research, and learn about what makes them excited to do what they do. I would also highly...eggs in one basket. There are many factors that can make or break a lab experience. Previous Addgene blogs...want to dedicate your time and energy to. How can I make the most of my experience in the lab? Ask your questions—especially... I’ve been afraid to ask “dumb questions” and to make mistakes. Ultimately, though, scientists are all...

...produced in the lab. The first decision you must make is which system you will use, or more specifically...specifically, the type of cells that will be used to make the virus. Viruses can be produced in many different... by the specific type of virus you are trying to make. For example, bacteriophages will need to be produced...methods, understanding the differences can help you make an informed decision. Mammalian cells Mammalian ... they contain some of the necessary machinery to make these viruses. The cells are also easy to cultivate...mammalian cells. However, it is much more common to make lentiviruses and AAV in-house. High-titer adenoviruses... that specifically infect insects. This is what makes insect cells a popular choice for protein production...