We narrowed to 166 results for: ipp

1. dTAG - You're it!

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   ...co-opt E3 ligases such as cereblon (CRBN) or von Hippel-Lindau (VHL) to bring the endogenous degradation...

2. RANbodies: Reporter Nanobody Fusions

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   ...stained with two HRP RANbodies if the sample is stripped. HRP-RANbodies are, however, compatible with most...

3. Visualizing Protein Turnover In Situ

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   ...dynamics of newly synthesized proteins in rat hippocampal neurons." Nature neuroscience 13.7 (2010): 897...

4. Human Germline Editing Using CRISPR

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   ...always come with some uncertainty. As biologists equipped to understand the results of this paper, we should...

5. RNA Extraction Without A Kit

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   ... mid 1980’s set out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski...

6. Antibodies 101: Selecting the Right Antibody

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   ...feel free to make the decision by price point, shipping time, or other factors. Just remember that these...

7. Transferable Skills Guide: Problem Solving

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   ...help convince a future employer that you are well equipped to work through challenges from day one in your...

8. Addgene: The First Twenty Years

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   ... our repository grew to over 150,000 plasmids, shipped out our two millionth item, and had a cumulative...

9. Plasmids 101: The protein expression toolbox

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   ...domains (DD) are akin to degron tags, just with a flipped mechanism. When a protein is tagged with a DD, ...

10. Developing Lab Management Software for Biology

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    ...technicians to retrieve the sample and get it ready for shipping. In the first case, we naively assume that everything...

11. New Tool for Lineage Tracing: The ClonTracer Library

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    ...work. She says, “I spend a lot of time packing, shipping, and sharing protocols one by one,” and she is...

12. Adenoviral Vector Production and Troubleshooting

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    ...personalized medicine (2017).   1st Gen AdVs are stripped of regulatory genes E1 and E3. Without these genes...

13. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...to divide. However, if fueled with caffeine and equipped with the pDCAF3 operon, the growth of this knockout...

14. Lab Automation at Addgene

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    ...vial fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials. Have you ever wondered...

15. Science communication: 9 Strategies to Get Your Foot in the Door

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    ...involved in education advocacy non-profits like Phipps Conservatory or the Brilliant Club and share your...

16. Hot Plasmids - October 2020

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    ... tracking the synaptic maturation pattern of hippocampal adult-born granule cells over time that were ...

17. Corralling and Cornering the Goldilocks Antibody

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    ... as showing a very bright signal only in the hippocampus compared to other regions, that informs us that...

18. Design Tips for Prime Editing

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    ...-1711-4 Chen, P. J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P.-F., Chen, C., Nelson...

19. Viral Vectors 101: Chemogenetics

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    ...immediate effects of specific neuron firing are better equipped using fast-acting optogenetic tools. The advantages...

20. Plasmids 101: Modular Cloning

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    ...piece. Rob is postdoc in Peter Schultz’s group at Scripps Research, where he works on genetic code expansion...