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Showing: 101 - 120 of 1106 results

  1. Qi Lab CRISPR Page

    Type
    Collection
    ...Individual plasmids can be ordered via the links below: ID Plasmid Description 44246 pdCas9-humanized A catalytically...Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts... hort P alindromic R epeats) pathway, as an RNA-guided DNA binding platform, to repress expression of ... Cas9 (dCas9) protein and a customized s ingle g uide RNA (sgRNA) designed with a 20-basepair complementary... are described in: Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene ...23452860 . PMC3664290 . CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert...

  2. AAV Packaged on Request

    Type
    Collection
    ... plasmid for more information on its performance. ID Plasmid Gene/Insert Promoter PI Additional Resources...transduce many mammalian cell types. AAV is used in a wide range of experiments, both in vitro and in vivo....

  3. Brain Initiative Collection

    Type
    Collection
    ...publications associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes...for in vivo use. Learn more about our viral service ID Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine...trusted partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications...of the brain generated through this effort will provide novel ways to treat, cure, and even prevent brain... the BRAIN Initiative below. Support has been identified by depositors and/or when BRAIN Initiative grants...jGCaMP8s and ChrimsonR are separated by cleavable peptide sequence P2A. Mark Histed 179459-AAV1 pAAV-EF1a-DIO-JEDI...available at Addgene. AAV Viral Service . Addgene provides a viral service to help facilitate scientific ...

  4. Genetic Code Expansion

    Type
    Collection
    ...contain one or more copies of the cognate tRNA gene. ID Plasmid Synthetase Origin ncAA Expression Codon PI...to enhance non-standard amino acid incorporation. ID Strain Description PI 48998 C321.ΔA all TAG sites...modified codons for unnatural amino acid incorporation. ID Plasmid Gene/Insert Vector Type PI...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...genetic code for all life is based upon four nucleotides, 64 codons, and 20 amino acids. Yet in the past...from the tRNA is incorporated into the growing peptide chain. To expand the genetic code, modified tRNAs... the genetic code, there are several things to consider. If you are working off of a previously established...

  5. Validated gRNA Sequences

    Type
    Collection
    ...Species Target Sequence Plasmid ID Application Cas9 Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG...in the associated article (listed below by PubMed ID). These factors include: Does a given gRNA sequence...gRNA Target Sequences You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...in Addgene's database as well as from sequences provided to us by users (see below for more details). Keep... Keep in mind that additional factors must be considered before using a validated gRNA sequence in your...

  6. EXPRESs plasmids for human platelets

    Type
    Collection
    ... 2015 May; 14(5):1265-74. PubMed 25713122 Plasmid ID Plasmid Name 51623 ACVR1-bio-His 51624 PECAM1-bio-His...screening of extracellular protein interactions to identify novel receptor-ligand interactions that function... A Human Platelet Receptor Protein Microarray Identifies the High Affinity Immunoglobulin E Receptor Subunit...

  7. Zebrafish Plasmid Collection

    Type
    Collection
    .... Large collections or kits are described below . ID Plasmid Description Gene/Insert PI Return to top ...rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater fish...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology ...CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression plasmids - Keith Joung...scientists in Latin America. The Zebrafish Book - A guide for the laboratory use of zebrafish. CRISPRz - A...zebrafish-specific gene expression database that provides information about when genes are active in developing...

  8. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...click on the plasmid name for ordering information. ID Name Promoter Description Category PI 157970 pTR-...application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable for...We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida...retina following subretinal injection. It was identified by John Chiorini and characterized by Shannon...

  9. Cre-lox system

    Type
    Collection
    ...of plasmids that are currently in our repository. ID Plasmid Description Promoter Expression System PI...Empty backbones to regulate your gene of interest: ID Plasmid Description Expression System PI 11578 pSico...selecting desired clones, and making transgenic mice. ID Plasmid/Article Description Expression System PI ... Cre; NeoR act15 D. discoideum Kay 108996 pDM1489 Cre; HygroR act15 D. discoideum Kay 109378 LZF40: LZF40...Cre expression once it is induced. Addgene has a wide variety of Cre recombinase plasmids. Special features...Cre expression plasmids. Addgene's viral service provides high-quality and high-titer viral preparations...65727 pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and ...

  10. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...Individual plasmids can be ordered via the links below: ID Plasmid Description 47948 3xFLAG-dCas9/pCMV-7.1 enChIP...Expresses a guide RNA (gRNA) to target human IRF-1 promoter 61080 gRNA-hIRF-1 #12/pSIR Expresses a guide RNA ...Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...genomic regions retaining molecular interactions and identify molecules interacting with those regions. Locus-specific...catalytically inactive form of Cas9 (dCas9) plus guide RNA (gRNA) for biochemical purification (Fig. 2)...interest. 98134 hIRF-1 gRNA #409 (Sa) Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the...system. 105283 hIRF-1 gRNA #351 (Sa) Expresses a guide RNA (gRNA) to target human IRF-1 promoter for the...

  11. TALEN Plasmids and Kits

    Type
    Collection
    ...Plasmids for Use with Addgene TALEN Kits For Use With: ID Plasmid Depositing PI Description Golden Gate TALEN...You may also like... Addgene's Genome Engineering Guide CRISPR Plasmids Zinc Finger Kits TALENs are important...repeats in the TAL effectors. View Addgene's TALEN Guide. These collections and accompanying documentation...

  12. Control AAV Preps

    Type
    Collection
    ...VCre-dependent Constitutive (non-cre-dependent) Clear Filters ID Name Promoter Fluorophore/Tag Activity Serotype PI...We provide high quality AAV preps from select plasmids in the repository. Browse our control AAV collection... found to match all requested criteria. Please consider using fewer filtering options. Browse all Control...

  13. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...Lab Improved Phosphoprotein Synthesis Reagents Type ID Item Strain 192872 rEcoli XpS (MG1655-C321 mutS+,... Human Serine Phosphopeptide Library described in: Encoding human serine phosphopeptides in bacteria for... for proteome-wide identification of phosphorylation-dependent interactions. Barber KW, Muir P, Szeligowski...Substrate Profiling Using a Proteome-wide Serine-Oriented Human Peptide Library. Barber KW, Miller CJ, Jun...serine phosphorylation ( Rinehart Human Serine Phosphopeptide Library ). The phosphorylation sites (“phosphosites...phosphoserine site flanked by 15 amino acids on either side occurring within the parent protein. Using the recoded...purifying the phosphosite library as GST-fusion peptides (“mode #1” expression). The mode #1 phosphosite...

  14. Rett Syndrome

    Type
    Collection
    ...bacterial) Insert species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return...toddlerhood primarily affecting females at an incidence of approximately 1:10,000. It is characterized...for proper nervous system function. While there is wide variability in symptoms even within patients with...window) PMID: 1606614 1999 - Huda Zoghbi's lab identifies MECP2 as the genetic cause of RTT. (Link opens...the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology ... Rett syndrome in Australia: a review of the epidemiology. J Pediatric . 148(3):347-352. (Link opens in...

  15. Optogenetics AAV Preps

    Type
    Collection
    ...AAV1 AAV2 AAV5 AAV8 AAV9 AAVrg PHPeB Clear Filters ID Name Promoter Opsin Variant Tag Activity Serotype...We provide high quality AAV preps from select plasmids in the repository. Browse our optogenetics AAV... See our Optogenetics Plasmids and Optogenetics Guide page for more information about this field. Use ... found to match all requested criteria. Please consider using fewer filtering options. Browse all Optogenetics...

  16. Retrograde AAV viral preps

    Type
    Collection
    ...Activators Inhibitors Other Molecular Tools Clear Filters ID Name Promoter Activity Category PI 37825 AAV-CAG-... found to match all requested criteria. Please consider using fewer filtering options. Browse all Retrograde...

  17. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Library Size Genome-wide Subpool Viral Prep Available Yes No Clear Filters Name ID Library Type Species...4096 (4 6 ) combinations of 6 nucleotides. Chong Zhang E. coli Genome-wide Inhibition Library 113134 — ...Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406 Human improved genome-wide library v1 ...genome-wide library v1 Discontinued Knockout Mouse Yusa 3rd 5 87,897 Mouse improved genome-wide library...Perturb-seq Guide Barcodes (GBC) 85968 Barcode Human Weissman 3rd N/A N/A Retroviral Mouse Genome-wide CRISPR...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...Adamson 3rd ∼3 366 or 1,573 Activity-optimized genome-wide library Discontinued Knockout Human Sabatini and...

  18. Biosensor AAV Preps

    Type
    Collection
    ...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Promoter Sensor Label Activity Serotype PI Acetylcholine... found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde...

  19. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ... R-mAbs from transfected mammalian cells. Addgene ID Plasmid Target Target Species Host Species Isotype...produce scFvs from transfected mammalian cells. Addgene ID Plasmid Hybridoma Target Target Species Host Species...nanobodies from transfected bacterial cells. Addgene ID Plasmid Hybridoma Target Target Species Host Species...IgG2a 188173 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/134R] NPY2R/Neuropeptide Y receptor type 2 Human...Parvalbumin Rat Mouse IgG2b 206639 Anti-Neuropeptide Y [L115/13R-2b] Neuropeptide Y Human Mouse IgG2b 206640 Anti-VGAT... 211594 Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/106R-2b] NPY2R/Neuropeptide Y receptor type 2 ...Rat Mouse 190505 NPY/Neuropeptide Y scFv [L115/13] L115/13 scFv 2t NPY/Neuropeptide Y Human Mouse 190506...

  20. Immunology Research Plasmids and Resources

    Type
    Collection
    ...CCN3, IGFBP9 NPFF neuropeptide FF-amide peptide precursor FMRFAL NPPA natriuretic peptide precursor A ANF... PIK3CD phosphoinositide-3-kinase, catalytic, delta polypeptide p110D PIK3CG phosphoinositide-3-kinase...natriuretic peptide precursor B BNP NPPC natriuretic peptide precursor C CNP NPR1 natriuretic peptide receptor... HBGF8, HBNF, NEGF1 PYY peptide YY PYY1 QRFP pyroglutamylated RFamide peptide 26RFa, MGC119794, P518 RABEP1...class I polypeptide-related sequence A FLJ60820, MGC111087, PERB11.1 MICB MHC class I polypeptide-related...
Showing: 101 - 120 of 1106 results