...flow strips that contain streptavidin which can bind to the biotinylated targets. They also tried to confirm...lateral flow strips. Since the probes are tagged with streptavidin, the lateral flow strip first captures...This post was contributed by Shravanti Suresh from Iowa State University. Since its appearance, SARS-... this virus has become an utmost priority for countries around the world and to do so, the WHO recommends...testing, tracking, and social distancing. With countries like South Korea, one of the earlier epicenters...be captured by an antibody further along on the strip. Highlights: Of the 83 known COVID-positive samples...viruses. Unlike Cas12a, Cas13a does not require strict sequence preferences at the target site, while ...

...originally being developed for A → G edits, researchers tried inhibiting MPG/AAG to improve editing efficiency...

...get them below a given threshold even when you’ve tried everything listed here. For some applications, this...CRISPR to (hopefully) be used in human clinical trials. An off-target rate of 5% is far too high when ...

...’s catalog — from the recently developed to the tried-and-true classics. Check out our Biosensors Collection...biosensors new to Addgene this year. Feel the love with MTRIA sensors One of the latest additions to Addgene’s...TRansmembrane region-to-IntrAcellular loop linkers” (MTRIA for short), where a fluorescent protein is inserted... ligand (Ino et al. 2022). The oxytocin sensor MTRIAOT enabled real-time observations of oxytocin dynamics...earlier tools. They also produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters...ligands - almost A to Z! - from ATP to urotensin. The MTRIA sensors will be useful for investigating neuronal...sensors for these and other targets. Figure 1: MTRIA biosensors are generated by inserting a circularly-permuted...

...GTPase, and GAP set is referred to as a minimal triad, with each component involved in GTPase signaling..., Gateway GTPase Sar1b 51128 198 Gateway GTPase Trim23 373 574 Browse ARF GAP and GEF Plasmids by Gene...

...zebrafish ( Danio rerio ), named for the horizontal stripes on the sides of the adult body, is a tropical freshwater...Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines from European researchers...

... Kit comprised of 834 plasmids of pre-assembled trimer and tetramer RVD domains designed for rapid TALEN...cell lines. Features include (i) Esp3I (BsmBI) restriction sites for full compatibility with the Golden ...

...caused by abnormal expansion of a repeating CAG triplet series within the huntingtin (HTT) gene. An individual... the disease, a small portion of cases can be attributed to known genetic factors such as defects in LRRK2...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...Goldstein Nematode: Injection and selection for Cas9-triggered homologous recombination Cas9-sgRNA target construct...

...TCTTCTTGTGCTCTTATTggcaggtacctggtgacTCACCTTATGGGCGCTGA matrixmetallopeptidase15 (membrane-inserted) TAL3484 & TAL3485 ...TGATCTGTTCCTGGTAGCcgttcatgagctgggcCATGCCCTTGGACTGGAA matrixmetalloproteinase14a (membrane-inserted) TAL3486 & TAL3487...

...Constitutive 9 Patriarchi 187180 pAAV_hSyn1_nLightR Syn nLightR none Constitutive 9 Patriarchi Oxytocin (OT...

...heard stories and read papers wherein a researcher tries 5 separate experiments at once to get confirmatory...procedures like pouring a plate or running a gel have intricacies that aren’t always mentioned in static written...

...Shortly after though, three groups independently tried reproducing these results and determined that even... expression. References: Allocca M, Doria M, Petrillo M, Colella P, Garcia-Hoyos M, Gibbs D, Kim SR,...

...Vector Core will produce viral-based vectors for distribution via Addgene. For a full list of Addgene's viral... The following vectors are now available for distribution at Addgene: Penn ID Addgene ID Name Function...

...engineering and expressing zinc finger proteins for distribution to academic and nonprofit laboratories for research...

... iNDI iPSC line generation, phenotyping, and distribution efforts. Plasmids The following plasmids can...

... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate...

... plasmids containing KLF, we encourage you to contribute to this community resource. Learn about the benefits...

...Collections Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection CRISPR CRISPR-on...

...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...