...Ehmee, and David Shiffman @WhySharksMatter and see what kind of content they share and produce and how they...nontraditional,” helped prepare me to start CINSO. Here, I share some of the tips I learned along the way and hope...about their research? Then we could get everyone to share their articles on social media and have their friends...their favorite. This seemed like a great way to share some of the research taking place at our university...in doing science outreach, not only can I easily share science with the public, but I can watch and learn...the point at which my career path began to take shape, was when I started volunteering. I wasn’t sure ...Additional Resources I asked some colleagues to share valuable life lessons they have learned during their...

...other blog posts on sharing science: 10 Ways to Share Your Science More Scientific sharing posts! ...Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate...first-year event’s success is evidence that, to share science, all you need is a great idea and a group...willing to make that idea a reality! How do you share your science with the community? Addgene would love... sharing...

...10 Ways to Share Your Science 6 Tools & Tips: Online Social Networking for Scientists Sharing Your Lab ...on the AMA process below, but we also wanted to share some thoughts on why we decided to do an AMA in ...reasons why you should consider using Reddit to share your science. While Reddit isn’t for everyone, particularly...well moderated. One of our original worries about sharing science advice on Reddit was that we’d be dragged...which falls in line with our general mission to share- as opposed to sell- science. 4. You can prepare... Before I turn it over to Joanne, I thought I’d share one of my favorite questions from the AMA - as you...Experiments Additional Resources See How We're Sharing Science through Video with the Addgene Youtube ...

...interest in the ways science is shared and in making it easier to share, we take special note as the open... to Share Science, in honor of a decade spent making plasmids more accessible and easier to share. As ...those of us at Addgene believe in the power of sharing, and especially in keeping the products of science...let's make a toast to open science. And, please, share your favorite OA developments of the past year with... comments. Or let us know your favorite ways to share science. Image credit: Judy Breck, via flickr ...

...thought leader in scientific sharing, leading by example to increase sharing in the community Addgene fosters...Ramsey, Randall, Ray, Rebecca, Renee, Shannon, Susanna, Tenisha, Thomas, Tony, Tracy, Tyler, Tyrel, Wogenie...transparency. We don’t have to explain that the materials shared through Addgene are being used for research purposes...we attract partnerships with organizations that share our community-oriented values. Addgenies like the...our mission to accelerate research with easier sharing and collaboration. Joanne and all the Addgenies...

...data if you need to. Similarly, FigShare also makes it easier to share data through their curation of ... at Harvard University 29:31 - 49:45 - Reagent Sharing - Susanna Bachle, Addgene the nonprofit plasmid...working to provide tools that make it easier to share in-depth information about reagents, protocols, ... Companies like Code Ocean allow researchers to share code they’ve used for data analysis while other ...organizations like Dryad make it easier for researchers to share data thereby enabling you to do modified analyses...science Twitter is very active with many scientists sharing their unpublished data. There are blogs - both ...ReFigure: Save Scientific Figures into Dashboards and Share Your Insights Who gives a tweet? 9 facts about scientists...

...Before I started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media...professionally, and 2) I needed to start using Twitter. Sharing Chemistry Cat memes use to be how I used social...used colony PCR before and thought it was cool to share my knowledge with other scientists who maybe hadn...paper. I wrote an Addgene blog post about it and shared the paper with my lab since one of the AAV vectors...of the #BLACKandSTEM Twitter community. Dr. Page shares her story of how one seemingly innocuous tweet ...the pointers are different from the other links shared in the post. And the blog writing tips are elements...media helped you advance your research or career? Share your story in the comments below!   Additional...

...to all of our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids (with 16 labs ...labs currently sharing viral preps) through the Addgene repository. Without these scientists and the support...to have such an impact on scientific materials sharing. I would also like to recognize a few scientists...Addgene has been successful in helping thousands of sharing scientists from laboratories all over the world...the scientists were waiting. I look forward to sharing new developments on this project and on all the...the interesting things going on at Addgene. Sharing speeds science and we are proud to be adding viral ...

...expression stable regardless of copy number (Segall-Shapiro et al., 2018). Designing stabilized promoters To...the case of the stabilized promoters from Segall-Shapiro et al., they chose a TALE protein as the repressor...replication, the stabilized promoters created by Segall-Shapiro et al. showed nearly no position-dependent differences...division slows (Kittleson et al., 2011). Segall-Shapiro et al. showed that the stabilized promoters were...during the transition to stationary phase, Segall-Shapiro et al. placed a trans-acting RepA protein under... proteins are needed sequentially. When Segall-Shapiro et al. placed the genes under the control of a ...stabilized promoters a try. References 1. Segall-Shapiro, Thomas H., Eduardo D. Sontag, and Christopher ...

...exchange vectors here! ShadowR, a novel FLIM-FRET acceptor Figure 4: ShadowR is used as a FRET acceptor...or mScarlet. Image: Murakoshi et al., 2019. ShadowR is an orange light-absorbing chromoprotein that...FLIM-FRET) in living cells (Figure 4). They created ShadowR by modifying the surface of the chromoprotein Ultramarine...binding to proteins can inhibit their activity. ShadowR can be used as a FRET acceptor in combination with...or mScarlet as the donor in HeLa cells.   Find ShadowR for mammalian cell expression here   Now that you...M., Sato, A., Koga, N., & Nabekura, J. (2019). ShadowR: a novel chromoprotein with reduced non-specific...

...., Gore, B. B., Weed, N., Omstead, V., Bishaw, Y., Shapovalova, N. V., Martinez, R. A., Fong, O., Yao,...expressed in both BALB/cJ mice and C57BL/6J mice (Shay et al., 2023; Huang et al., 2023). Systemic capsids...CNS: neurons and astrocytes Mice No (LY6C1) Shay et al., 2023 AAV-PHP.V1* CNS: Biased toward...cells in the CNS of both BALB/cJ and C57BL/6J mice (Shay et al., 2023). AAV-PHP.AX is biased toward astrocytes...would also like to thank Viviana Gradinaru and Tim Shay for their support and input. Resources and references...Bindu, D. S., Zhang, M., Taskin, N., Goertsen, D., Shay, T. F., Sullivan, E. E., Huang, S.-F., Ravindra ...X., Lefevre, A., Singh, V. P., Arokiaraj, C. M., Shay, T. F., Vendemiatti, J., Jang, M. J., … Gradinaru...

...include materials sharing in their dissemination plans and in particular, that they all share the plasmids ...scientists from 21 different labs who clearly also share this wonder of the organism.   It’s not an overstatement...ways to culture, propagate, transform, store, and share all of these new organisms continues to be challenging...systems. How the EDGE Program promotes scientific sharing The EDGE Program is designed to expand the number...The parasitic fusion is such that the two plants share RNA, proteins, and DNA. Euprymna scolopes (Joshua...re excited to see new tools being developed and shared for many different organisms. Do you have a favorite...

... what challenges remain in materials sharing, information sharing, and research reproducibility while ...to reflect on our accomplishments from 2022 and share what we’re looking forward to in 2023 and beyond...2022 we also rolled out an updated AAV Data Hub to share curated information about AAV usage in research ...around the world can upload AAV validation data, sharing what works and, equally importantly, what doesn...commitment to supporting the research community through sharing science and distributing high-quality materials...

...resources References Shemetov, A.A., Oliinyk, O.S. & Verkhusha, V.V. How to Increase Brightness of Near-Infrared...4557 (2017). Shcherbakova, D.M., Baloban, M. & Verkhusha, V.V. Near-infrared fluorescent proteins engineered...Shcherbakova, D.M., Stepanenko, O.V., Turoverov, K.K. & Verkhusha, V.V. Near-Infrared Fluorescent Proteins: Multiplexing...Shemetov, A.A., Pletnev, S., Shcherbakova, D.M. & Verkhusha, V.V. Smallest near-infrared fluorescent protein...Nat Methods 11, 641-644 (2014). Filonov, G.S. & Verkhusha, V.V. A near-infrared BiFC reporter for in vivo...Shcherbakova, D.M., Cox Cammer, N., Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and...Nat Commun 5, 3626 (2014). Shcherbakova, D.M. & Verkhusha, V.V. Near-infrared fluorescent proteins for multicolor...

...openness and sharing in science.  We’re excited to support the neuroscience community by sharing not only ...neuronal manipulation. The goal of the workshop was to share the results of these discussions as a publicly accessible...To complement these efforts, we’re beginning to share data through our recently launched AAV Data Hub,...researchers using AAV from our viral service can share experimental design and data. With these different...science, we aim to accelerate the rate by which sharing speeds science.   Check out the precise neuronal...

...in Dr. Zhang's introduction. His willingness to share reagents so freely with the academic community has...a puzzle to decrypt to a tool to be harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition...repository, and I was proud to be responsible for sharing some of our data at this meeting. We weren't quite...gratitude for the role Addgene plays in plasmid sharing to those who were anxious to hear about what was...have given the attendees an amazing opportunity to share their cutting edge science in increasingly important...Interested in Addgene's Keystone Poster?  We'll be sharing the poster with our blog readers and on social ...

...very anxious at this point. Here's the message I shared with the Addgenies on March 11, 2020 after rumors...This is a time when we need as much open science sharing as we can get. The effects of Addgene’s brief...for grant-driven research being done in all labs sharing materials via Addgene. Perhaps if each of many ... unique resource for scientists that helps them share reagents — leading to open, reproducible science...pandemic-related slowdown. More scientists are sharing plasmids and other reagents every day, and this..., and we look forward to supporting scientific sharing of materials for the foreseeable future. ...

...research in terms of preparedness, organization, and sharing data. Here are five FREE science lab apps that ...is a multipurpose application that allows you to share your data with colleagues and link your notebook...the information about that restriction enzyme or share it via email with a colleague. The app is also great... Quiz/Card by Andrey Solovyev Lab Help by Kris Shamloo These are just a few of the many apps available...the lab. If you have a favorite lab app, please share it in the comments section below! Many thanks to...Notebook for Bioinformatic Analyses Use Apps to Share Your Lab Protocols Check Out How Addgene Develops...

...for supporting scientific sharing. We’re very excited for more reagent sharing projects in the years to...15 years of plasmid sharing has certainly taken us on many adventures...From moving office locations ...service in 2016...we’re excited to help scientists share their reagents with the scientific community.   ... tools. How many times have these plasmids been shared in total? Just over 49,000 times! Their long-lasting... from the Zhang lab. In fact, the Zhang lab has shared over 40,000 CRISPR-Cas9 components over the years...

...presentations and a handout on protocol sharing, material sharing, computational reproducibility, and tools...wet lab protocols, reagent sharing, bioinformatic tools, data and code sharing, and data analysis and visualization...who were also thinking about reproducibility and shared similar desires to take action. Thanks to Teytelman...s contributions by Tyler Ford included reagent sharing and were presented at the ASPB meeting by the collaborators...