...Two hybrid systems were developed in Saccharomyces cerevisiae in 1989 and are still used extensively ...

...majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along the way involves...

...Sadowski. 1985. “The FLP Recombinase of the Saccharomyces Cerevisiae 2 Micron Plasmid Attaches Covalently...

... vectors for CROPseq-multi using selection by Puromycin, Zeocin®, or nuclear expression of BFP2.   ...

...check out these two references: Dayton, Robert D., Mychal S. Grames, and Ronald L. Klein. "More expansive...

... a mScarlet reporter, luciferase reporter, and neomycin resistance reporter. Image adapted from Katti ...

...So, think beyond Drosophila melanogaster or Saccharomyces cerevisiae (both fine research organisms) -  ...

...et al. "Adaptation of Enterococcus faecalis to daptomycin reveals an ordered progression to resistance....

...Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae. Paques F and Haber JE. Microbiology...

...genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS Yeast Res. 12(2) (2012): 197...

...protein‐tracking studies in the budding yeast Saccharomyces cerevisiae." Yeast 25.9 (2008): 651-659. PubMed...

...Toolkit for Extensive and Flexible Engineering of Saccharomyces Cerevisiae.” ACS Synthetic Biology, vol. 12,...

... genes along with 1000 non-targeting controls Puromycin Doench , Root 73179 Human gRNA pooled library ... genes along with 1000 non-targeting controls Puromycin Doench , Root Human activation pooled libraries...56,762 unique sgRNAs targeting 18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries...human genes along with 500 non-targeting controls Puromycin Doench , Root 92386 Human CRISPR inhibition library...human genes along with 500 non-targeting controls Puromycin Doench , Root Mouse knockout pooled libraries ... genes along with 1000 non-targeting controls Puromycin Doench , Root Cas9 Lentivirus Cas9 viruses can...Zhang 61426 lenti MS2-P65-HSF1_Hygro MS2-P65-HSF1 Hygromycin lenti vector encoding the MS2-P65-HSF1 activator...

...plasmids is described in: Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Dicarlo...meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin 48668 M-SPcas Mammalian S. pyogenes Cas9 expression...pyogenes Cas9 (SP) + tracrRNA expression, cloDF13/spectinomycin 48647 DS-ST1cas Bacterial S. thermophilus #1...#1 Cas9 (ST1) + tracrRNA expression, cloDF13/spectinomycin 48648 DS-TDcas Bacterial T. denticola Cas9 (... (TD) + tracrRNA expression, cloDF13/spectinomycin 48649 PM-SP!TA Bacterial SP crRNA expression: targets...

...Control Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination... tested for mycoplasma contamination. To date, Addgene has never had a case of mycoplasma contamination...contamination using mycoplasma detection kits. The cell line is maintained for ~20 passages before being...

...Resources The m echanistic or m ammalian t arget o f r apamycin (mTOR) is a key metabolic regulator controlling...threonine kinase 11 mTOR Mechanistic target of rapamycin NF1 Neurofibromin 1 PRAS40 Also known as AKT1S1...associated protein 1 mTOR Mechanistic target of rapamycin p53 TP53; tumor protein p53 PDK1 Pyruvate dehydrogenase...10.1016/j.cell.2012.03.017. PubMed PMID: 22500797 . Rapamycin: one drug, many effects. Li J, Kim SG, Blenis ...

...YET Cre is coexpressed with tdTomato and Pac (puromycin N-acethyl-transferase) pCDH-CB-copGFP-T2A-iCre...pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato...-copGFP-T2A-Puro 72263 Express copGFP and the puromycin resistance gene from the EF1 promoter pCDH-CMV-mCherry-T2A-Puro...pCDH-CMV-mCherry-T2A-Puro 72264 Express mCherry and puromycin resistance gene from the CMV promoter pCDH-CMV...

...-pLKO-puro 3rd inducible expression of shRNA; puromycin selection. See plasmid 21916 for Neo selection...cDNA expression, gateway plasmid, constitutive Hygromycin resistance gene coexpression. See article for...19319 pLJM1-EGFP 3rd for EGFP fusion; PGK driven puromycin Sabatini 25895 pLX301 3rd Gateway plasmid for ...Tet-pLKO-neo 3rd inducible expression of shRNA; neomycin selection. See plasmid 21915 for Puro resistance...

...pyogenes URA3 Wyrick pCRISPomyces-2 61737 Bacteria BbsI yes, cut S. pyogenes Apramycin Zhao pRB1017 59936...none S. pyogenes Zon pCRISPomyces-1 61736 Bacteria BbsI yes, cut S. pyogenes Apramycin Zhao pMB70 47943 Worm...activate, interfere, or nick. Selection , such as Puromycin or EGFP Cloning enzyme used for insertion of your...

... Helicobacter pylori Listeria monocytogenes Mycobacterium sp. Neisseria sp. Pseudomonas sp. Ralstonia ...application and by color. External Resources European Saccharomyces Cerevisiae Archive for Functional Analysis (...Reagent Program (Link opens in a new window) Saccharomyces Genome Database (SGD) (Link opens in a new window...