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Showing: 101 - 120 of 424 results

  1. AAV for Neuronal Tracing

    Type
    Collection
    ... Neuronal Tracing References Wickersham IR, Finke S, Conzelmann KK, Callaway EM. 2007a. Retrograde neuronal...Wickersham IR, Lyon DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, Young JA, Callaway EM. 2007b. Monosynaptic...

  2. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...staggered cutter  Originally classified as Cpf1, Cas12’s primary difference from Cas9 is that it generates ...j.molcel.2019.03.014 Zetsche, Bernd, HGootenberg, S., Jonathan, Abudayyeh, O., Omar, et. al. Cpf1 is a....2015.09.038 Harrington, B., Lucas, Burstein, David, Chen, S., Janice, et. al. Programmed DNA destruction by miniature...

  3. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...657. Douglas Kim , GENIE Project Calcium GCaMP6f/m/s calcium sensors for imaging neural activity (Constitutive...and mRuby2 for imaging neural activity (GCaMP6f/m/s) (Constitutive or Cre-dependent) Cell-specific restoration...S0896-6273(20)30398-6. Edward Boyden Calcium GCaMP6f/s Ca sensors and separate nuclear localized dTomato ...Calcium Intersectional viral expression of GCaMP6m/s in cells depdent on Cre, Flp, or Vcre Comprehensive... Calcium Axon-specific calcium imaging (GCaMP6f/m/s) (Constitutive or Cre-dependent) In vivo measurement... encoded fluorescent sensor. Proc Natl Acad Sci U S A. 2004 Dec 14;101(50):17404-9. Ca2+ indicators based...genetically encoded sensors. Proc Natl Acad Sci U S A. 2011 May 3;108(18):7351-6. Amy Palmer Zinc Cytosolic...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    Blog Post
    ...thanks to our guest blogger, Harshana S De Silva Feelixge. Harshana S De Silva Feelixge is a researcher whose...This post was contributed by guest blogger Harshana S De Silva Feelixge. Gene therapy technologies hold...science communication.     References 1. Gaspar, H. B., S. Howe, and A. J. Thrasher. "Gene therapy progress ...

  5. Plan Your Experiment

    Type
    Collection
    ...endogenous gene(s) without permanently modifying the genome dCas9-activator (such as dCas9-VP64) gRNA(s) targeting... (CRISPRi) Reduce expression of a particular gene(s) without permanently modifying the genome dCas9-repressor...repressor (such as dCas9-KRAB) or dCas9 gRNA(s) targeting promoter elements of target gene dCas9-KRAB ...

  7. Year of the Camelid: Antibody Style

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    Blog Post
    ...10.3389/fimmu.2017.01589 Jin B, Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation...https://doi.org/10.3390/ijms24065994 Xu J, Xu K, Jung S, et al (2021) Nanobodies from camelid mice and llamas...

  8. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM...

  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...180. PubMed PMID: 11815306. 4. Nicole Kresge, R. D. S. and R. L. H. “The Discovery of Avidin by Esmond E... (2004). PubMed PMID: 20353954. 5. Kay, B., Thai, S., & Volgina, V. “High-Throughput Biotinylation of ...PubMed PMID: 2388593 7. Holden, M. A., & Cremer, P. S. “Light activated patterning of dye-labeled molecules...Central PMCID: PMC4461904. 9. Huang, Q., Mao, Z., Li, S., Hu, J., & Zhu, Y. “A non-radioactive method for ...PMC4439073. 12. Ludwig, L. B., Hughes, B. J., & Schwartz, S. A. “Biotinylated probes in the electrophoretic mobility...

  10. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Mahou P, Le Franc Y, Supatto W, Matho KS, Ieng S, Fouquet S, Dupin E, Benosman R, Chédotal A, Beaurepaire...technique to subdivide neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson ...Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature...Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development...

  11. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...Stohler P, Bocquet N, Hug MN, Huber S, Siegrist M, Hetemann L, Gera J, Gmür S, Spies P, Gygax D, Geertsma ER...Breedam W, Roose K, van Schie L, Hoffmann M, Pöhlmann S, Graham BS, Callewaert N, Schepens B, Saelens X, McLellan...Egloff P, Hutter CAJ, Kuhn BT, Bräuer P, Newstead S, Dawson RJP, Geertsma ER, Seeger MA (2020) Generation...

  13. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...produce the primary recombinant adenoviral stock (rAdV-S). HEK293 cells are transfected with the AdV plasmid...The resultant lysate is your primary low titer rAdV-S, and can be stored at -80C for later use or used immediately...very low titer. Amplification (1-2 weeks) - The rAdV-S is used to infect more HEK293 cells and produce more...8892868. PubMed Central PMCID: PMC190817. 5. Lee, Cody S., et al. "Adenovirus-mediated gene delivery: Potential...

  14. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...10.1016/j.jconrel.2019.02.009 Wang D, Mou H, Li S, Li Y, Hough S, Tran K, Li J, Yin H, Anderson DG, Sontheimer...the plasmids at Addgene: pAdSh.PGK.Cas9 (expresses S. pyogenes Cas9 from the PGK promoter) and U6 promoter-driven...Elsevier, pp 39–67 Ding Q, Strong A, Patel KM, Ng S-L, Gosis BS, Regan SN, Cowan CA, Rader DJ, Musunuru...

  15. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...When complexed with a full-length gRNA, native Cas9's two catalytic domains, HNH and RuvC, cleave DNA strands... to dramatically alter levels of expression. Cas9’s ability to alternately serve both as a means of modifying...gRNAs An alternative approach is to modulate Cas9’s nuclease activity by modifying the gRNA with which...nucleotides of complementarity to a target site, Cas9’s DNA binding capability remains intact while its nucleolytic...

  16. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    ...they think they are.  References Berns KI, Adler S (1972) Separation of two types of adeno-associated...Maynard LH, Smith O, Tilmans NP, Tham E, Hosseinzadeh S, Tan W, Leenay R, May AP, Paulk NK (2019) Fast-Seq... . https://doi.org/10.1089/hgtb.2019.110 Pacouret S, Bouzelha M, Shelke R, Andres-Mateos E, Xiao R, Maurer...

  17. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
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    ...technology! Resources and References References Orguc, S., Sands, J., Sahasrabudhe, A., Anikeeva, P., & Chandrakasan...https://doi.org/10.1109/EMBC44109.2020.9175600 Park, S., Loke, G., Fink, Y., & Anikeeva, P. (2019). Flexible...C8CS00710A Sahasrabudhe, A., Rupprecht, L. E., Orguc, S., Khudiyev, T., Tanaka, T., Sands, J., Zhu, W., Tabet...

  18. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...Bright, S. M., Robinson, M. K., Graham, E. T., Sabo, J. P., Owens, R., King, D. J., Norris, S. H., & Scher... Scher, D. S. (1994). Isotype choice for chimeric antibodies affects binding properties. In Journal of...

  19. Plasmids 101: TOPO Cloning

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    Blog Post
    ... colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia virus DNA topoisomerase...coli is sequence specific."  Proc Natl Acad Sci U S A.  1991 Nov 15;88(22):10104-8. PubMed PMID: 1658796...synthesis using vaccinia DNA topoisomerase. Shuman S. J Biol Chem.  1994 Dec 23;269(51):32678-84. PubMed...

  20. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...out, might depend more on the individual sequence(s) than on how well that sequence fits into the five...Knight, T. F., Thomas C. Evans, J., & Lohman, G. J. S. (2018). Comprehensive Profiling of Four Base Overhang...Potapov, V., Bilotti, K., Pokhrel, N., & Lohman, G. J. S. (2022). Rapid 40 kb Genome Construction from 52 Parts.... B., Bilotti, K., Cantor, E. J., & Lohman, G. J. S. (2020). Enabling one-pot Golden Gate assemblies of...
Showing: 101 - 120 of 424 results