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Learn about antibody engineering, including what it is and why we like it. Deep dive into antibody directed evolution techniques, and learn about Addgene's partnership with the Institute for Protein Innovation (IPI).

Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The collection can be used for screening transcription factors for cellular programming or cancer drug resistance.

High schoolers invented a new diagnostic for Lyme disease. In the process, they also created a tool for jointly designing RPA primers and Cas12a sgRNAs.

Learn about RNA interference and how you can use shRNA plasmids to knockdown gene expression!

This guide on single chain fragment variables (ScFvs) covers how ScFvs are made, the advantages and disadvantages of using ScFvs, and several applications.

Learn how CRISPR-based diagnostic systems can easily detect nucleic acids of choice with high sensitivity, specificity and portability.

Learn how you can use kinase translocation reporters to monitor kinase activity at the single cell level.

Choosing a CRISPR activator? This article features four of the most popular CRISPR activators (dCas9-VP64, SAM, SunTag, and VPR) and some newer activators like dCas9-CBP and SPH.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

Multicistronic or bicistronic vectors contain IRES and/or 2A peptides to allow for coexpression of multiple genes from a single transcript.

How do we know if a therapeutic antibody is a promising clinical prospect? In vivo and in silico methods can help us decide!

Repositories provide fast access to wet lab research materials during COVID-19 such as cell lines, plasmids, and mouse strains.

Ginkgo Bioworks and Addgene are synthesizing and distributing thousands of plasmids for COVID-19 research. Read to learn more about these constructs

Check out how you can use the top lentivirus and AAV from 2016 in your own research.

Check out some of the new and useful tools recently deposited in the Addgene repository!

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

Looking for a plasmid that can thrive in a number of different bacteria? Learn about broad host range plasmids in this post!

If flow plots seem confusing, our guide to understanding them is here to help!

Open science resources like Addgene's Data Hub increase reproducibility, reduce validation and optimization timeframes, and drive science forward.