...Get started with Gibson Assembly Cloning!...plasmids by designing primers to split an antibiotic resistance gene to effectively create an extra part, one...contiguous DNA fragment. Pro-Tip Add Extreme Thermostable Single-Stranded DNA-Binding protein (ET SSB)...

...the SV40 origin of replication use a HEK293 line stably expressing the SV40 large T antigen. For antibody-expressing...Epstein-Barr virus origin of replication use a HEK293 line stable expressing Epstein Barr nuclear antigen (EBNA).... saline solution, Millipore Sigma A6279 Before Starting Warm the DNA and working stock of PEI-MAX to room...

... out more protocols and videos to help you get started in the lab! Introduction There are four biosafety...sometimes scientists work for a long time while standing. Wear comfortable shoes that cushion your feet...

...entire construct. Is the primer an appropriate distance from the target? The primer should be a minimum...and make sure they are justifiable peaks. For instance, in the trace file below, you can see that just...

... out more protocols and videos to help you get started in the lab! Introduction A centrifuge is a common...balanced. Using the centrifuge in an unbalanced state can damage the centrifuge and be dangerous for the...

.... (2014) (Link opens in a new window) . Before Starting To reduce the risk of contaminating reagents we...0.5 2 50 Annealing/Extension 60 1 2 50 Signal Stabilization 98 10 2 1 Hold 4 ∞ 2 1 After PCR is complete...

...properties: Length of 18-24 bases 40-60% G/C content Start and end with 1-2 G/C pairs Melting temperature (...

...DNA are denatured. Pro-Tip Do not vortex at this stage or the genomic DNA will become sheared and will ...