Colony Formation Titering Assay
Type
Protocol
...markers. Note this assay requires staining resistant colonies with crystal violet solution and, therefore...Change media as needed Days 14–18: Stain cells and count resistant colonies Equipment Class II, Type A2...Filter 0.1% crystal violet solution through a 0.22 μm filter to remove any precipitates. Stain each well...well with 1 mL of 0.1% crystal violet for 10 min at RT. Gently remove the stain. Wash cells 3x with 1 mL...exchanges every 3–4 days. Colonies were then stained with 0.1% crystal violet and counted. Figure 2: A549 cells...lentiviral preparations that confer antibiotic resistance. Protocols...lentivirus preparations that confer antibiotic resistance. Determining the titer of your lentiviral preparation...