Viral Vectors 101: Viral Vector Elements
Type
Blog Post
...what goes into ‘making virus’ or what gag and pol stand for, we demystify all components of viral vectors...
We narrowed to 1,055 results for: sta
-
-
Viral Vectors 101: Biosensors
TypeBlog Post...Biosensors tend to be more reproducible when used as stable systems, rather than transiently overexpressing... -
GPCRs: How Do They Work and How Do We Study Them?
TypeBlog Post...//doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, ... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog Post...OptoNB can’t bind, it localizes to the cytosol. To start, the team inserted AsLOV2 into conserved sites in... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post...a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line and then transduce this... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...if not methylated (if it comes from a phage for instance). Aside from restriction modification systems,... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...workshop was well received, and then when the team started brainstorming ideas for the next workshop, they... -
Summer SciComm: Preprints
TypeBlog Post....org) in the past few years. Figure 1: Stand aside, CERN — it's a new age of accelerating science... -
Plasmids 101: Golden Gate Cloning
TypeBlog Post...method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in different combinations, or other... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away does. ... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...organism where deletion strains are available. Then, staff or students clone similar sequences from a less-... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...aa 155), includes I152L in N-terminal fragment Constant rate of p53 tetramerization in response to DNA... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...useful tools, and as such, we’re always looking to understand our community better. We therefore looked at ... -
Viral Vectors 101: Voltage Indicators
TypeBlog Post...translate perceived signals to physical actions. Understanding neuronal pathways and the function of certain... -
xCas9: Engineering a CRISPR Variant with PAM Flexibility
TypeBlog Post...where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM range, recognizing... -
Plasmids 101: 5 factors to help you choose the right cloning method
TypeBlog Post...techniques do tip the scales in your favor. For starters, Golden Gate cloning is extremely efficient due... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...time and resources allow, you might be better off starting from the beginning. Before trying again, revisit...