...if not methylated (if it comes from a phage for instance). Aside from restriction modification systems,...

...workshop was well received, and then when the team started brainstorming ideas for the next workshop, they...

...medRxiv.org) in the past few years.   Fig. 1: Stand aside, CERN - it's a new age of accelerating science...

...method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in different combinations, or other...

...organism where deletion strains are available. Then, staff or students clone similar sequences from a less-...

...2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used...

...aa 155), includes I152L in N-terminal fragment Constant rate of p53 tetramerization in response to DNA...

...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...

...away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away does. ...

...techniques do tip the scales in your favor. For starters, Golden Gate cloning is extremely efficient due...

...where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM range, recognizing...

...useful tools, and as such, we’re always looking to understand our community better. We therefore looked at ...

...translate perceived signals to physical actions. Understanding neuronal pathways and the function of certain...

...and adaptations that can provide an increased understanding of DNA binding and gene regulation. For additional...

...not a leader,” because you don’t see yourself  standing in front of a crowd and using your charisma to...

...extremely important gene in animal development. To understand where sonic hedgehog got its name, we need to...

...medical journal. Decades later and after years of painstaking work trying to isolate the causative gene for...

...IgG isotype standard 2.5 mg/mL - can use commercial standard or validate an in-house standard Before Starting...Thaw IgG standard and prestained protein ladder on ice. Reagent Preparation Prepare IgG Standard Dilute ...ready-to-use bio-safe Coomassie G-250 stain (Addgene uses SimplyBlue SafeStain) and ImageJ software. The sample... serial dilutions of a standard antibody of known concentration. After staining with Coomassie blue, protein...ready-to-use biosafe Coomassie stain and ImageJ software. Protocols... Protocols Coomassie Purity Stain of Recombinant Antibodies...Antibodies Coomassie Purity Stain of Recombinant Antibodies You may also like... Antibody Plasmid Collection...

... Protocols Generating Stable Cell Lines with Lentivirus Generating Stable Cell Lines with Lentivirus... antibiotic resistance (e.g., puromycin, blasticidin), which enables selection of a stable cell culture...lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells but can be adapted... of antibiotic, which may not be stable at 37 °C. To achieve a stable cell pool, the antibiotic selection...expression. As polyclonal populations of resistant cells start proliferating and the individual wells become...Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector...Introduction This protocol can be used to generate stable cell lines expressing a gene of interest from an...

...help stabilize the standards add carrier DNA to a final concentration of 4 ug/mL to each standard dilution... Once a validated standard curve is obtained, make a small aliquot of each standard (enough for 1 or 2...their initial value. If the Ct value of the standard starts to drift, it’s time to make a new one. When...your plasmid standard with DNase ** Make 6 serial dilutions, in duplicate, of your standard curve plasmid...5'-CGGCCTCAGTGAGCGA ITR-containing plasmid for standard curve RNase-free DNase 10X DNase buffer Nuclease-free...Pipette tips General Considerations Always run standards and samples in duplicate at least If possible,... the samples have been added to the qPCR plate. Start by adding water, then SYBR master mix, then the ...