We narrowed to 1,053 results for: sta

1. Viral Vectors 101: Biosensors

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   Blog Post

   ...Biosensors tend to be more reproducible when used as stable systems, rather than transiently overexpressing...

2. GPCRs: How Do They Work and How Do We Study Them?

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   Blog Post

   ...//doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, ...

3. OCaMP: A New Calcium Indicator for Neural Imaging

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   Blog Post

   ... GCaMP series (e.g., jGCaMP8) remains the gold standard for green fluorescence, while red indicators jRGECO1a...

4. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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   Blog Post

   ...that this enzyme does not cleave at a predictable distance from the targeted region. Konermann et al. show...

5. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ...a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line and then transduce this...

6. Plasmids 101: Methylation and Restriction Enzymes

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   Blog Post

   ...if not methylated (if it comes from a phage for instance). Aside from restriction modification systems,...

7. Summer SciComm: Preprints

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   Blog Post

   ....org) in the past few years.   Figure 1: Stand aside, CERN — it's a new age of accelerating science...

8. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   Blog Post

   ...workshop was well received, and then when the team started brainstorming ideas for the next workshop, they...

9. Plasmids 101: Golden Gate Cloning

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   Blog Post

   ...method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in different combinations, or other...

10. Viral Vectors 101: Chemogenetics

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    Blog Post

    ...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...

11. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post

    ...2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used...

12. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    Blog Post

    ...aa 155), includes I152L in N-terminal fragment Constant rate of p53 tetramerization in response to DNA...

13. Progress Towards a PAM-Free CRISPR

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    Blog Post

    ...away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away does. ...

14. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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    Blog Post

    ...organism where deletion strains are available. Then, staff or students clone similar sequences from a less-...

15. Viral Vectors 101: Voltage Indicators

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    Blog Post

    ...translate perceived signals to physical actions. Understanding neuronal pathways and the function of certain...

16. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ...useful tools, and as such, we’re always looking to understand our community better. We therefore looked at ...

17. Plasmids 101: 5 factors to help you choose the right cloning method

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    Blog Post

    ...techniques do tip the scales in your favor. For starters, Golden Gate cloning is extremely efficient due...

18. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post

    ...where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM range, recognizing...

19. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ...and adaptations that can provide an increased understanding of DNA binding and gene regulation. For additional...

20. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post

    ...not a leader,” because you don’t see yourself  standing in front of a crowd and using your charisma to...