...Master Mix 2X Primer pair targeting AAV2 ITR ( Aurnhammer et al., 2012 ) fwd ITR primer, 5'-GGAACCCCTAGTGATGGAGTT...10 μL 1,000 μL 100 μM Forward Primer 0.15 μL 15 μL 100 μM Reverse Primer 0.15 μL 15 μL Nuclease Free Water...GGAACCCCTAGTGATGGAGTT rev ITR primer, 5'-CGGCCTCAGTGAGCGA ITR-containing plasmid for standard curve RNase-free... SYBR master mix, then the forward and reverse primers. Vortex briefly, immediately before use. Use a ... of ~70–75 °C usually indicates the presence of primer dimers which can increase background signal and...

...features to help with the post-design process such as primer and plasmid design. Examples:  “multiplex design...or multiplex features. However, tools aiding in primer design are more and more common. User interaction...

...on the Addgene blog: Addgene Optogenetics guide Primer on Optogenetics blog series Addgene Biosensors ...

...chatting quickly with the postdoc who’s really good at primer design – but first, you have to know what resources...

...amenability to genetic manipulation.  This is just a primer so that you are aware of at least some of the possibilities...

...amplified using the same sets of forward and reverse primers). The abundance of a particular barcode sequence...

... Addgene Blog New to Virus Work? Check out Our Primer for Beginners Read Our Lentivirus FAQ Learn about...

...or toe clip) and using PCR with the appropriate primers to detect your targeting vector or gene of interest...

...Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics    ...

...stuff: the experiments! In our final post in this primer on optogenetics we’ll delve into some of the important...

...epitope tag or fusion protein. If your sequencing primer was chosen to confirm a tag or fusion protein is...

... region of interest can be PCR amplified using primers that (A) flank the region of interest (deletions...

...expressed is amplified using a gene-specific forward primer containing the desired restriction site (the MCS...has several options) and a pUCX-specific reverse primer containing the missing codons from the antibiotic...if a small contaminating PCR product formed from primer dimers and containing the missing codons from the...resistance gene is inserted. It is important that primers be designed to minimize the possibility of dimer...

...Adenovirus Associated Virus New to Virus? Check out Our Primer for Beginners! Learn All about Lentiviral Vectors...

...effective. Their Snapdragon tool helps you select primers to synthesize RNA for gene silencing. Additionally...

...and how they work, watch Endy's 10-minute YouTube primer on BIL gates.) Addgene: Why is it so important ...

...process. Either SYBR green technology or a TaqMan primer/probe set can be used and samples are quantified...

... plate containing the mastermix which includes primers and a ddPCR supermix. Note that the supermix and...

...half a century; the Addgene blog has an excellent primer and tips from experts on FRET techniques. By combining...

...determining titers by qPCR, we recommend using primers targeting the viral genome or inverted terminal...