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We narrowed to 132 results for: primer

Showing: 81 - 100 of 132 results

  6. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Master Mix 2X Primer pair targeting AAV2 ITR ( Aurnhammer et al., 2012 ) fwd ITR primer, 5'-GGAACCCCTAGTGATGGAGTT...10 μL 1,000 μL 100 μM Forward Primer 0.15 μL 15 μL 100 μM Reverse Primer 0.15 μL 15 μL Nuclease Free Water...GGAACCCCTAGTGATGGAGTT rev ITR primer, 5'-CGGCCTCAGTGAGCGA ITR-containing plasmid for standard curve RNase-free... SYBR master mix, then the forward and reverse primers. Vortex briefly, immediately before use. Use a ... of ~70–75 °C usually indicates the presence of primer dimers which can increase background signal and...

  7. Sequencing Primers

    Type
    Guide
    ...what primers are listed under "5' sequencing primer" and "3' sequencing primer" or explore the sequence ...list of universal sequencing primers and other common sequencing primers. Educational...Molecular Biology Reference Sequencing Primers Sequencing Primers Though Addgene mainly uses next-generation...sequencing primers. These lists are provided for your reference, Addgene does not distribute primers. To identify...additional annotated primers. You can also use Addgene’s protocol to design your own primers . Still have questions...plasmid verification, Addgene has used a number of primers for Sanger sequence verification over the years...years. Below is a list of popular universal primers available from most sequencing services, along with a...

  14. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...Vectors? Learn about Viral Vector Elements Read Our Primer on Optogenetics    ...

  15. Molecular Biology Reference

    Type
    Guide
    .... For a list of commonly used primers, check out our sequencing primers page . Types of Plasmids Plasmids...enzyme, the template DNA to be copied, and a primer. A primer is a small piece of DNA, approximately 18–...Molecular Cloning Techniques Promoters Sequencing Primers Origins of Molecular Genetics The concept of genes...sort the cells by visualization or flow cytometry. Primer Binding Site A short, single-stranded DNA sequence...amplification or DNA sequencing of the plasmid. Primers can be utilized to verify the sequence of the insert...know some of its sequence to design a effective primer. To learn more about the sequencing technologies...approximately 500–1,000 bases downstream of the known primer region with very few errors, making it an efficient...

  19. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...expressed is amplified using a gene-specific forward primer containing the desired restriction site (the MCS...has several options) and a pUCX-specific reverse primer containing the missing codons from the antibiotic...if a small contaminating PCR product formed from primer dimers and containing the missing codons from the...resistance gene is inserted. It is important that primers be designed to minimize the possibility of dimer...
Showing: 81 - 100 of 132 results