...posits the way science is being done has changed significantly over the last decade. He describes the detection...these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up...

...months, pausing or terminating employment for a significant fraction of the workforce. Wet lab scientists...exponential decay model, with dips during weekends and significant holidays. Figure 4: Plasmid request trajectories...

...ancestry, Sean and Aaron knew that their study had significant statistical limitations, and expected to find...Cas9 from cutting, both of which can introduce significant bias into the results. But the reference genome...

...GFP positive cells isolated with TREE have a significantly higher frequency of genomic base pair edits ...GFP positive cells can be sorted and have a significantly higher frequency of base edits of interest than...

... channel as the Cas13 reporter), they could significantly amplify the detection signal for a single target...but is undergoing validation studies.     Jennifer Tsang contributed to updating this article. References...

...expressing it with additional cofactors or using significantly truncated forms that limit its functionality...Both PILATeS (18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors (~60 ...

... the Addgene repository can save researchers significant time and removes a variable from the lengthy ...trends in our own repository data, giving us significant insight into which vectors are likely to be of...

...Ward T.H., Margison G.P. “Mycoplasma infection significantly alters microarray gene expression profiles.”...Ellem K.A., Cleghorn G., West M.J., Wei MQ. “The significance of controlled conditions in lentiviral vector...

...survey respondents indicated that cost was a significant barrier to ELN adoption. This includes financial... step of the research process will save them significant amounts of time and effort and give the lab a...

...has spread to almost every part of the world manifesting as a full-fledged pandemic. Containing the spread...emission reader Approach: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique that uses...

...evolution until they identified a mutant with significantly increased sensitivity that did not affect the...any issues in toxicity. TurboID’s signal is significantly greater than miniTurbo at earlier time points...

...are not exclusive to IF, but they can have a significant impact on the outcome of your IF experiment. ...antibodies you choose for your experiment will have a significant impact on the protocol you use and the ultimate...

...tdTomato), they produce a temperature sensor that significantly outperforms previous designs. B-gTEMP is excitable...optimal conditions). While B-gTEMP itself is a significant improvement over previous temperature indicators...

...mice, it is sex-independent and does not show significant differences in transduction efficiency between...brain. Both AAV9 and AAV9.452sub.LUNG1 have significantly higher off-target expression in the liver than...This post was written by Rachel Leeson, with significant contributions from Cynthia Arokiaraj. Addgene...

...results, the signal from your target needs to be significantly higher than the background noise (Waters 2009....14112. PubMed Central PMCID: PMC5773976. Waters, Jennifer C. “Accuracy and Precision in Quantitative Fluorescence...

...of interest. The piggyBac™ system allows for significantly enhanced random integration efficiencies that... If the cell’s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates...

...Identifying new ligands for orphan GPCRs is of significant medical interest, however efforts to do so have...improved version of BioID, called BioID2, is significantly smaller, allows for more selective targeting...metabolically cooperating (SeMeCo) communities. The uniform multiple cloning site in the plasmid series also...

... Organizing a thoughtful engaging talk takes significant effort. It requires evaluation of all of the ...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...

...repair of a temperature-sensitive pha-1 mutation significantly enriches for knock-in of 2x and 3xFLAG epitopes...recipients for the 2015 Breakthrough Prize included Jennifer Doudna and Emmanuelle Charpentier for their pioneering...

... amplicon sequencing (Hendel et al. 2015), a significant difference as CRISPR experiments move closer ...networks in biological systems. NGS will also play significantly into the realm of clinical therapeutic development...