...labels for the core parts that would be found on almost any centrifuge. Types and Parts There are several...techniques. Regardless of the type of centrifuge you use, most will have essentially the same components. The photo...

...Cloning? No need for specific restriction sites. Join almost any two fragments regardless of sequence. No scar...contiguous DNA fragment. Pro-Tip Add Extreme Thermostable Single-Stranded DNA-Binding protein (ET SSB)...

...contains the plasmid DNA separated from bacterial chromosomes. Collect the supernatant into a new tube by pipetting.... This is included in the resuspension buffer of most kits. Reference Page Top Index...

...green are the ones loaded onto the qPCR plate for most samples. If sample is expected to have a titer <...have some small amount of background signal that is most evident during initial PCR cycles. This background...

...Although our recommended temperature should kill most potential contaminants, certain types of spores ... agar will remain liquid at this temperature but most antibiotics will not break down at this temperature...

...is. While simple homogenization is effective for most mammalian tissues; more hardy tissues such as bone...

...SMAP, SMAP2, p120 BTC betacellulin - C19orf10 chromosome 19 open reading frame 10 EUROIMAGE1875335, IL25... CD27L, CD27LG, TNFSF7 CECR1 cat eye syndrome chromosome region, candidate 1 ADGF, IDGFL CER1 cerberus...

...different wavelengths of light to varying degrees and most have a specific wavelength that they maximally absorb...

...concentration of long-term glycerol storage is unknown. Most labs store bacteria in 15-25% glycerol. You can ...

...and room temperature. This general protocol suits almost any water bath. Video Watch the video for tips ...

...best buffer that works for both of your enzymes. Most companies will have a compatibility chart, such ...

...manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your...

... placing it in the PCR machine. Diluting Primers Most people order primers from a company, which synthesizes...

...of dilutions and pick the population that has the most desirable level of expression. Over time, transgene...

...manufacturer’s instructions for your competent cells. For most standard cloning, you can transform 1-2μl of your...

...protein of interest. RIPA buffer is suitable for most proteins but more stringent buffers and a sonication...

...place of the stuffer. The AgeI site is destroyed in most cases (depending on the target sequence), while ...

...genome. They have four chromosomes: the X (and Y), second, third, and fourth chromosomes. Also, the fourth ...third chromosomes are the ones you’ll work with. If YFG is somehow on the Y or fourth chromosomes, you ...geneticists use balancer chromosomes to prevent genetic recombination. Balancer chromosomes essentially are messed...the start of the day and throughout daytime. They mostly stop emerging at night. An incubator with a light...fourth and Y chromosomes are miniscule, so researchers generally do not manipulate them. Essentially, the X... messed up chromosomes with multiple inversions. They are unable to participate in genetic recombination... viable if they are homozygous for a balancer chromosome. Thus, you can stably maintain a heterozygote...

...tropicalis, the two most commonly used species of frog. (Liu et al., 2016). Some of the most popular tools ...emerging model organisms!) Escherichia coli One of the most recognizable and utilized model organisms is Escherichia... organism for over 60 years with a status as the most studied prokaryotic organism. With its fast growth...low cost, it’s understandable why E. coli is the most popular bacterial organism in microbiology and biochemistry...developed to thrive in laboratory settings. One of its most attractive features is its stability and easily ...Youn, 2016). For these reasons rats were once the most used mammalian model in medical research with an...has its perks, it is far from perfect. One of the most limiting attributes of rats is their difficult to...

...assay. The most common options are 4 °C overnight or 1–2 hours at room temperature (RT). Most people choose...need depends mostly on the weight of the proteins you’re interested in. Table 1 lists the most common types...wet transfer is the most efficient method, meaning that it will transfer the most protein from your gel...probably the most limiting of steps, as you may only have access to one or another. Most institutes have...blot protocol and protocol video!  Antibodies The most important part of your western is your antibodies...direct detection method Indirect detection is the most common way to run a western blot. It allows you ...different lysis buffers available commercially, and most common buffers have recipes available online if ...