...the risk of contaminating reagents we recommend making small aliquots of master mixes, primers, and probes...and place in the Biological Safety Cabinet (BSC). Make sure that the BSC to be used for dilution and the...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...

...place it in the Biological Safety Cabinet (BSC). Make sure that the BSC is supplied with sufficient pipette...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...the risk of contaminating reagents we recommend making small aliquots of master mixes, and primers/probe...

...repeated throughout a specific DNA sequence, which can make it difficult at times to identify compatible restriction...site-specific recombination or a ligation step, making it an easy, cheap and rapid cloning method. So ...

...Over-Agar Antibiotic Plating You may also like... Making LB Agar Plates Bacterial Transformation Recovering...transformed E. coli . Over-agar spreading of antibiotic makes it easy for an investigator to conveniently plate...

... Rule For each sample you want to load on a gel, make 10% more volume than needed because several microliters...For example, if you want to load 1.0 μg in 10μL, make 1.1μg in 11μL. Reference Page Top Index...

...sequencing primers, including: Is the primer unique? Make sure the primer only anneals once within the entire...incorrect sequence. Look at the peaks in the area and make sure they are justifiable peaks. For instance, ...

...exhibited high levels of constitutive activity, making them less than ideal. DREADDs D esigner R eceptors... previously used in human populations in Japan, making it an attractive option for translational studies...

... that increase the transformation efficiency and make bacteria more susceptible to either chemical or ... plasmids at once. To save money, many labs also make their own competent cells. This is a relatively ...

...endonuclease negative E. coli strain such as NEB stable. Make a mixture of a total of 500 μL PEI-OptiPro SFM with...-Tip There can be batch to batch variation when making the PEI working stock, therefore the ratio of μg...

...plunger. Remove the entire pipette from the container making sure not to touch the sides of the container with...important for scientists to calibrate their pipettes to make sure that they are properly tuned to dispense the...

...solutions at various dilution. Make sure to use the polybrene-containing media to make the cell solution in this...

...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...

...buffer. Fill the chamber with 1X MOPS running buffer. Make sure that the chamber is sealed. Load 5 µL of the...Pro-Tip Leaving clear lanes between samples will make quantifying the gel easier. Cover the electrophoresis...

...bottle up/down vigorously for 30 sec (it’s okay to make bubbles). Incubate at RT for 15 min. Note that longer...add the OptiMEM + DNA + PEI solution to the CS5. Make sure that all five layers are covered with media...

.... See below for options if the recombinant band makes up a significant proportion of the DNA pool. Last... weight of each pellet should be ~1-2 g. Pro-Tip Make sure to weigh the empty tube beforehand! If you’...

...the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace is composed ...known mutants at $7.00 a strain. Makerspaces like ours can also make accounts with  Addgene. We now have...of the aspects of this project that makes it ideal for makerspaces is the data is freely available, and...  Funding Makerspaces One of the main challenges when starting a new project at a makerspace is finding...following post was contributed by Derek Jacoby from Makerspace Victoria, CA Over the decades science has become...manifested itself in the creation of Hackerspaces and Makerspaces back in 2007, which function as centres for peer...lectures. There are currently around 1,000 active makerspaces around the world. Hackerspaces.org maintains ...

...experience and make sure your next team works better. In fact, don’t wait to be invited onto a team. Make one yourself...Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d spent my senior year ... For example, when it came time to update how we make and distribute kits, I helped assemble a crack team...Is the goal of your project to launch a product? Make an informed decision about something? Fix a problem...indeed. Mission team A Mission Team is assembled to make progress towards open-ended goals whose metrics ...Team, a like-minded group of co-workers who want to make our organization as environmentally friendly as ...There’s also no end-game here, unless we manage to make Addgene the most environmentally friendly organization...

...they don’t make in wages, they make up for in training. Unfortunately, these low wages also make it so it... trainees pay may also make them more attuned to the bang for their buck - make them demand more services...answered about the makeup and welfare of the many researchers out there who are making great progress on...academic research community to develop a plan to make postdoctoral pay/benefits commensurate with work...how many post-docs there are in the U.S., their makeup (in terms of nationality, gender, race, etc), or... One of the Saturday Hack Day projects sought to make an interactive game that highlights just this point...will help you in your academic publishing goals and make you a better communicator overall. Don’t Write...

...reasoning out the window when making decisions. We think scientists make choices based on logic and reason...’s actually something you CAN choose and it will make a huge difference for your future career and life...Which route should I take to get to work?”; we often make important life decisions without much logic - even...your own style of work and communication before making this important decision. I’ll highlight some of...matter to me?”  and hone in on the factors that will make the most difference for your success. Gather data...of written questions for the PI and lab members.  Make coffee or beer dates to talk to as many lab alumni...energy level in the lab? Does a frenetic atmosphere make you edgy or energized?   Are you an only child and...

...online). Mentee: Make a list of topics/skills you are interested in exploring. Mentor:  Make a list of topics...your career development as your mentor. How do you make the most of this new relationship? Consider adding...take this past the chatting stage you will rarely make any change in your skill sets or activate the helpful...that need work or growth. Here are some ideas for making your meetings productive: Discuss logistics if...communicate between meetings (email, phone, etc.)? Make agendas for your meetings, take informal minutes...goals with a mentor: The secret of accountability To make a mentoring relationship really productive, the ...many mentoring meetings to hone the goals and to make them “SMART.” SMART goals are Specific, Measurable...