... Antibody Suggestion Form . Browse All Antibodies ID Recombinant Antibody Description Source Species Isotype...based on our in-house testing and data provided by trusted outside labs – with full experimental details...accessibility in research. For each antibody, we provide important details including the protein and plasmid...about antibodies and their use, visit our Antibody Guide . Browse or search the table below or visit the ...you choose, store, or use your antibody. Antibody Guide Antibody Protocols Recipient Instructions Antibody...

...information on the experimental use of these plasmids. ID Plasmid Gene/Insert Vector Type PI Publication Do...Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog...was originally used. Here are a few questions to consider when choosing a gRNA: Does a given gRNA sequence...the deposit process. Want more information on the wide variety of Cas enzymes? CasPEDIA is an encyclopedia...enzyme activity, PAM requirements, experimental considerations, and more, created and maintained by the Doudna...

...sequencing Plasmids Available as Cloning Grade DNA ID Plasmid Description Industry PI FAQ What Quality ... about Addgene's Beta Projects and how you can provide us with input and feedback on this service. DNA...and Customer Support Teams are also available to provide assistance at [email protected] . Don’t See What...

...2011 Nov 9;480(7378):534-7. PubMed 22080952 Plasmid ID Plasmid Name 36145 ama1CD4d3+4-bio 36146 ama1CD4d3...screening of extracellular protein interactions to identify novel receptor-ligand interactions that function...

...target that guide the user through the REAL or REAL-Fast assembly process or lists of plasmid ID numbers ...and the DNAs produced by all three methods are identical in sequence. TAL effector repeat arrays produced... a new window) is available to enable users to identify both dimeric TALEN and monomeric TALE transcriptional...additional pages describing these reagents are provided below: Joung Lab REAL Assembly TALEN Kit Individual...

...transferred to Addgene for distribution. Look up the IDs of particular Penn Vector Core catalog items. Virus-associated... experimental conditions. Protocols Written and video protocols for how to use your viral prep and how... Addgene, with details about storage. Biosafety Guide Documentation for your institutional biosafety office...biosafety of viral vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from...used to make the virus. In order for Addgene to provide viral vector services to you, we must ensure that...

...allow you to insert and induce your gene of interest. ID Plasmid Description Co-expressed tTA, rtTA, or TetR...expresses the transactivator for your tetraycline system. ID Plasmid Description Element On or off PI 26429 pLenti...expression. Several endogenous promoters had been identified that responded to stimuli, such as hormones or... lac system with the inducer isopropylthio-β-galactoside (IPTG) was tested first, but IPTG was found to... Element (TRE) A TRE is seven repeats of a 19 nucleotide tetracycline operator (tetO) sequence, and is...domain, resulting in reduced gene expression. This idea of a hybrid transactivator was initially used with...1995, Gossen et al. used random mutagenesis to identify which amino acid residues of tetR were important...

... AAV preparations of many chemogenetics plasmids. ID Plasmid Description Gene/Insert Vector Type Promoter...Plasmid Collection You may also like... Chemogenetics Guide Viral Service: Chemogenetics AAV Blog: Chemogenetics... Actuator Modules (PSAMs). DREADDs are the most widely used chemogenetic receptors and are G-coupled protein... your experiments, please see our Chemogenetics Guide . Chemogenetics Plasmids Browse, sort, or search...

...Individual plasmids can be ordered via the links below: ID Plasmid 46294 pBS-Hsp70-Cas9 : A codon-optimized ...Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts...site sequence can be added by inserting an oligonucleotide linker into BsaI digested vector. Cloning targeting...additional information CRISPR Optimal Target Finder Identifies gRNA target sequences from an input sequence ...Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease. Gratz SJ, Cummings AM, Nguyen JN...

... ) VPR ( Addgene #63798) Plasmids from Collection ID Plasmid Description PI Publications Kaufman, M. L...phosphorylated oligonucleotides containing the guide target sequence: Up to two guides can be expressed...utilizing one of the dual guide shuttle plasmids to insert a second guide within a U6-guide cassette. This process... shuttle plasmid for shuttling U6-guide cassettes to make dual guide expressing plasmids dCas9-KRAB-MeCP2...desalted oligonucleotides with the appropriate overhangs: The plasmids listed here are provided as empty...Protocols Single guide cloning: Zhang Lab General Cloning Protocol (PDF, 237 KB) Dual guide cloning: Brzezinski...in the context of the developing mouse retina. To aide in their studies of the regulatory networks involved...

...plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s) Description PI 16539 pBV-Luc...plasmids; these are noted with the viral service icon . ID Plasmid Luciferase Type Promoter Description PI 60226...that contains your regulatory element of interest. ID Plasmid Description Gene/Insert PI Return to Top ...emission of photons. The robust signal, sensitivity, wide dynamic range, and resistance to photobleaching ...depending on the application. Firefly luciferase is considerably brighter than Renilla luciferase, but is more...systems. Firefly and Renilla luciferase are less-than-ideal protein tags due to their large size. NanoLuc® Luciferase...level of expression. Scientists have developed a wide range of luciferase-based plasmid systems for many...

... such as pX458 (Addgene plasmid ID 48138) or pX459 (Addgene plasmid ID 48139), which include GFP and puromycin... the pSpCas9(BB) plasmid (pX330) (Addgene plasmid ID 42230). This plasmid allows for the simultaneous ... . Use these tools to help identify guide sequences that minimize identical genomic matches or near-matches...CRISPR Plasmids CRISPR Guide Addgene Blog Posts Addgene is proud to present a video reprint from the Journal...oligos for each guide and its associated reverse complement including additional nucleotides for cloning ...Add “CACC” before the 20-mer guide sequence and “AAAC” before the guide’s reverse complement for cloning...Journal of Visualized Experiments (JoVE). The video publication by Stuart Orkin's and Daniel Bauer's labs...

...items will be shipped as stabs at room temperature. ID Name 13421 pAC-Kan-alphaGal4 13422 pBAC-lacZ 13424...

...available in standard format and as a Lentiviral prep . ID Plasmid Description Mutations Industry PI Due to ...

...site", "active site loop", or "subunit interface") ID Plasmid Description Gene/Insert Mutations Additional...protein with many available isoforms and mutants provides a shared experience for the research community...

...virus. AAV Vectors for Monosynaptic Neuronal Tracing ID Name Description Serotype PI 52473 pAAV-synP-FLEX-splitTVA-EGFP-B19G...Rabies Virus-based Monosynaptic Tracing The core idea of the system is to infect the cell population of...deletion-mutant rabies, the essential G protein is provided to the starter cell in trans , via delivery by...

...antibodies (search for "anti-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top ... encoding scFv fragments that bind to the GCN4 peptide, from the popular SunTag system, fused to sfGFP...antibodies and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following...

...synthases needed for use of these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector...Bergendahl V, Marahiel MA.1998. Peptide bond formation in nonribosomal peptide biosynthesis. Catalytic role...MCP tagged plasmids for visualization of proteins inside cells. Depositor...ACP-, and MCP-tags for visualization of proteins inside living or fixed cells. The plasmids associated ...-cell-permeant proteins. CLIP-tag CLIP-tag is identical in function to SNAP-tag but reacts with benzylcytosine...are useful for labeling secreted proteins with disulfide bridges such as antibodies. MCP-tag is a mutant...

...AAV5 AAV8 AAV9 Retrograde AAV PHPeB Clear Filters ID Name Receptor and Activity Reporter/Tag Cre requirement...See our Chemogenetics Plasmids and Chemogenetics Guide pages for more information about this field. Receptor... found to match all requested criteria. Please consider using fewer filtering options. *AAVrg = retrograde...

...Individual plasmids can be ordered via the links below: ID Plasmid Description 44246 pdCas9-humanized A catalytically...Available from Addgene You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts... hort P alindromic R epeats) pathway, as an RNA-guided DNA binding platform, to repress expression of ... Cas9 (dCas9) protein and a customized s ingle g uide RNA (sgRNA) designed with a 20-basepair complementary... are described in: Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene ...23452860 . PMC3664290 . CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert...