...interneurons with AAV-S5E2-GCaMP6f are now available as a ready-to-use viral prep in serotype AAV1. Distinguish...lab are now available as ready-to-use AAV vectors in serotype AAVrg.   ...putative pre-synaptic terminals with one vector. AAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby from Liqun ...cortical interneurons. We now provide ready-to-use AAV vectors expressing GCaMP6f with this promoter. Image...Distinguish dendritic neurites from axonal neurites with AAV vectors expressing cytoplasmic targeted tdTomato ...pre-synaptic targeted synaptophysin tagged eGFP. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE from Hongkui...of GCaMP8 vectors in serotypes AAV1 and AAV5. AAV9 preps and more AAV1 versions will be released soon....

...sighted readers.    Figure 1: An image from AAV Purification by Iodixanol Gradient Ultracentrifugation...image in Figure 1 appears on our protocol page for AAV Purification by Iodixanol Gradient Ultracentrifugation...vectors for the first time and learning how to purify AAV.  Second, you’ll identify your purpose in using that...the technical details of collecting the purified AAV–that the interfaces between layers are blurred after...are captured at the 40% layer. To collect purified AAV, insert a syringe at the interface of the 40% layer...

...specifically AAV, are also being exploited as delivery mechanisms for gene therapy. The tractability of AAV, low...of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided on an application, our...00487-6 Additional Resources on the Addgene Blog AAV: A Versatile Viral tool for Gene Expression in Mammals...Listen to Connie Cepko Discuss Gene Therapy with AAV Adenoviral Delivery of CRISPR/Cas9 Aims to Expand...

...and adeno-associated virus (AAV). The least risky of these four viruses is AAV – it is not known to cause...integrate at chromosome 19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety...safety level. Adenovirus is similar to AAV in that it does not integrate into the host’s genome. However...bleach (plastic) or ethanol (other surfaces). For AAVs, which are resistant to ethanol and isopropyl alcohol...

...optogenetic experiments. Intracranial injections of AAV are commonly used to deliver channelrhodopsins to...collected following intracranial injection of an AAV expressing a ChRger. In both experiments, neurons...Designer channelrhodopsins paired with a systemic AAV allow for minimally-invasive optogenetics While ChRgers...delivered ChRgers by intravenous injection of PHP.eB, an AAV capsid capable of broad transduction of the nervous... on the Addgene blog Read more about the PHP.eB AAV capsid Read more about optogenetics Learn about the...

...Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA arrays...platforms: transfection, lentiviral transduction, and AAV transduction.  In most of Zetsche et al’s experiments...array that can be processed by Cpf1. Lastly, an AAV vector was used to multiplex edit 3-4 genes in a ...experiments, cells were infected with a 1:1 ratio of two AAVs,  one expressing Cpf1 and the other a crRNA array...Experiment A Match Made in Heaven: CRISPR/Cas9 and AAV Resources on Addgene.org Browse Our CRISPR Multiplexing...

... it is too large to be effectively packaged into AAV particles for delivery into mammalian cells, especially...archaea are much smaller and would be ideal for such AAV packaging, but their activity in mammalian systems... Cas12a based systems, making it well suited for AAV packaging. Most importantly, this CasMINI system ...effective at genome editing when delivered in an AAV system, and demonstrated that the new system was ...methods, including plasmid, ribonucleoprotein, and AAV.  Read the paper in Nature Chemical Biology Find...

...expression for AAV CREATE libraries Gradinaru 206504 AAV-KP1 AAV-KP1 AAV packaging vector containing AAV2 rep and...codon Mali 166921 AAV-F in pAR-9 AAV-F AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F Maguire 170716...McCown 183749 kiCAP-AAV-BI30 AAV-BI30 AAV-BI30 Rep-Cap plasmid for production of AAV-BI30, a capsid with...-KanR AAV-BI28 RepCap for AAV production Deverman 203533 iCAP-BI-48-KanR AAV-BI48 RepCap for AAV production...-KanR AAV-BI49 RepCap for AAV production Deverman 203535 iCAP-BI-62-KanR AAV-BI62 RepCap for AAV production...and partial AAV2 p5 promoter Kay 206512 AAV-LK03 AAV-LK03 AAV packaging vector containing AAV2 rep and LK03...and partial AAV2 p5 promoter Kay 206513 AAV-AM AAV-AM AAV packaging vector containing AAV2 rep and AM ...

...Collection Addgene's AAV Data Hub More resources on the Addgene blog Newly Updated AAV Data Hub Antibodies..."Pass". Curating the data reports Just like our AAV Data Hub, each data submission we receive is checked...

...service provides ready-to-use AAV and lentiviral preps! Download the green AAV background.       Some of...the image background.     If you’re working with AAV or lentivirus in your research, you might want to... and needed a break so they're hanging out with Aavery in this Zoom video background. Want the purple ...bacteria video background with mascots Blugene and Aavery? Download the 720p version or download the 1080p...

...also rolled out an updated AAV Data Hub to share curated information about AAV usage in research applications...applications. Scientists around the world can upload AAV validation data, sharing what works and, equally importantly...

... AAV8, as well as AAV5 and AAVrg. Find the Cre-expressing viral preps here AAV-CAG-dLight1.3b from ...in serotype AAV5! Get dLight1.3b here Looking for a knock-out in your experiments? AAV-flex-taCasp3...available as a ready to use viral prep in serotype AAV5. Find AAV-flex-taCasp3-TEVp here The red-shifted channelrhodopsin...SaCas9 ABE variants. It's also one of the smallest AAV-deliverable ABE.  Find the plasmid here Jesse Zalatan...the Deisseroth viral preps here Coming soon! New AAV serotypes from the Sanford Boye lab with enhanced...include AAV1, and AAV9. Find viral preps here pAAV-hSyn-Cre-P2A-dTomato is now available in AAV1 and AAV8...Loren Looger Lab now available in AAV1. Get GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature...

...elements are inserted into an AAV transfer vector and used to generate AAV particles. Transient or stable...for conducting genome-wide screens using CRISPR. AAV transduction Only compatible with SaCas9 (packaging...or gRNA. Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery....hard Viral delivery   Lentivirus Retrovirus AAV Cells Dividing or nondividing Dividing Dividing...

...future experiments.  Use Addgene’s AAV Data Hub. You can search the AAV Data Hub for tools to use in future...experiments. If you’ve used Addgene’s AAV tools, you can contribute data to the AAV Data Hub to help the research...

...Addgene.org AAV Vector for TVA Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...viruses typically used in neuroscience research (e.g., AAV) are useful for labeling the individual neurons they...17179932. Additional Resources on the Addgene Blog AAVs CREATed for Gene Delivery to the CNS and PNS Advanced...

...currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful...tricks in our blog post “Droplet Digital PCR for AAV Quantification.”   Multiplex PCR Multiplex PCR, as... nucleotide concentration of starting material AAV Titrations Allelic Variations Quantifying low abundance...resources on the Addgene blog Droplet Digital PCR for AAV Quantification Plasmid cloning by PCR  Plasmids 101...

...Packaging Service AAV Retrograde AAV Viral Vector Packaging Service: Retrograde AAV As part of our Viral...catalog items. Browse Available Retrograde AAV See our retrograde AAV based on the functional catagories listed...Promoter Activity Category PI 37825 AAV-CAG-GFP CAG GFP Control Boyden 51502 AAV pCAG-FLEX-EGFP-WPRE CAG EGFP...sensor Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium sensor Kim 104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE...104493 pGP-AAV-syn-FLEX-jGCaMP7b-WPRE Syn GCaMP7b, Cre-dependent Calcium sensor Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE CAG GCaMP7f, Cre-dependent Calcium sensor Kim 104488 pGP-AAV-syn-jGCaMP7f-WPRE...104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE Syn GCaMP7f, Cre-dependent Calcium sensor Kim 162374 GP-AAV-syn-jGCaMP8s-WPRE...

...distributing a small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents... lab from the Allen Institute have supported the AAV initiative from the beginning and have worked closely...Research Campus have provided valuable insight into AAV production. Finally, we are blessed to have an incredibly...Blog Check Out the Top Requested Lentivirus and AAV of 2016 Learn How You Can Use the Viral Service in...

...Broad tropism - AAV-PHP.eB, AAV-PHP.AX, AAV-PHP.eC Cell type-biased: Astrocytes - AAV-PHP.AX with GRE ...Neurons - AAV.CAP-B10, AAV-PHP.N Broad - AAV-PHP.AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP....and NHPs: Broad tropism - AAV-MaCPNS2, AAV-PHP.S Sensory nervous system - AAV-MaCPNS1 Return to top...pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-...GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488 pGP-AAV-syn-jGCaMP7f-WPRE...scientific community. Text Description for the AAV Decision Tree The AAV decision tree graphic above helps you ...

...vectors. Jonathan Ting 172907-AAV1 pGP-AAV-syn-FLEX-Volton2-ST-WPRE AAV-mediated expression of voltage sensor...GENIE Project 172908-AAV1 pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE AAV-mediated expression of voltage...Addgene's AAV are made from a selected set of plasmids that are currently in our repository. AAV are distributed...coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA Ian Wickersham 104052-AAVPHP.V1 pAAV-CAG-DIO-EYFP An AAV genome...Viral Prep Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven...enhancer element Gordon Fishell 99120-AAV1 pAAV-ihSyn1-tTA An AAV genome that expresses tTA from the hSyn1...of the tTA Viviana Gradinaru 99121-AAV1 pAAV-ihSyn1-DIO-tTA An AAV genome with Cre-dependent expression...