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Showing: 81 - 100 of 882 results

  1. Plan Your Experiment

    Type
    Collection
    ... containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning ...agarose gel to identify DNA fragments. PCR and restriction digest (for HDR repaired DSBs): For small nucleotide...nucleotide edits that introduce a novel restriction site. The region of interest is PCR amplified, digested...digested with the appropriate restriction enzyme and run on an agarose gel to identify DNA fragments. PCR...

  2. Bacterial Expression Systems

    Type
    Collection
    ...protein resources here . pCyPet-His 14030 FRET Patrick Daugherty FRET: Vector for arabinose inducible ...YPet) found in pYPet-His . pYPet-His 14031 FRET Patrick Daugherty FRET: Vector for arabinose inducible ... and P. pastoris . All plasmids use the same restriction enzymes for cloning so multiple constructs can...compatible with standard cloning using many different restriction enzymes. pPro24-gfp contains GFP but all others...

  3. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...Plasmids for REAL (Restriction Enzyme And Ligation) TALEN assembly containing single TALE repeat domains...any desired sequence, referred to as the REAL (Restriction Enzyme And Ligation) assembly method ( Sander...encoding a single TALE repeat domain and flanked by restriction sites used for ligation into longer arrays. These...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ...reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent stem cell reprogramming...conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells. Proc Natl Acad Sci U S...Spiny Neurons Lentiviral Human Generation of Human Striatal Neurons by MicroRNA-Dependent Direct Conversion.... 2017 Aug;9(8):1117-1131. Parmar Fibroblasts Dentritic Cells Lentiviral Mouse Direct reprogramming of...

  5. AAV Viral Preps

    Type
    Collection
    ... viral vector preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality...genome sequencing (VGS). In-stock AAV preps are distributed as 100 µL solutions of purified viral particles...Samples Small samples of control AAV vectors for trial purposes in serotypes AAV1, 2, 5, 8, 9 and retrograde...

  6. CRISPR Guide

    Type
    Collection
    ...activity with endogenous cell-type specific miRNAs to restrict CRISPR editing only to the target tissue. Browse... Acidaminococcus sp. ). The popularity can be attributed to Cas12a’s many benefits. Cas12a exhibits increased... quantification and can be used on lateral flow strips. Other Cas enzymes can be added to SHERLOCKv2 to...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations...924–927. PMID: 30377362 Casini, A., Olivieri, M., Petris, G., Montagna, C., Reginato, G., Maule, G., Lorenzin... (12), 770–788. PMID: 30323312 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward, C. C., Levesque, S....152 (5), 1173–1183. PMID: 23452860 Savic, D., Partridge, E. C., Newberry, K. M., Smith, S. B., Meadows...

  7. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...as in-stock preps. These high-titer preps are distributed as 100 µL solutions of purified viral particles...Vector Core have been transferred to Addgene for distribution. Look up the IDs of particular Penn Vector Core... have the right to use the original plasmid. Distribution of the original plasmid is important because...

  8. Microbiology Resources

    Type
    Collection
    ...microbiology resources. The plasmids Addgene distributes cannot be used to reconstitute self-replicating...Addgene continues to monitor its library and distributions in accordance with export laws and biosafety...Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium sp. Deinococcus radiodurans Enterococcus sp. ...

  9. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... Other/Tribolium castaneum BsaI none S. pyogenes Averof p(U6b-BsaI-gRNA) 65956 Other/Tribolium castaneum...encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity, experimental considerations... pKS diaCas9_sgRNA 74923 Other/Phaeodactylum tricornutum U6 yes, cut S. pyogenes Winge pJJR50 75026 Worm...

  11. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...was first described in 1966 by Austrian physician, Andreas Rett, who tried to raise awareness and published...binds to methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson & Bird...in which too much of the MECP2 protein is as detrimental as too little, resulting in MECP2 duplication...advance their respective gene therapies to clinical trials in 2021. Figure 1: Schematic of the human...

  12. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...for most purposes, but what do you do when you’ve tried a common strain and don’t get the desired level ...inactivated native restriction/methylation system. This means the strain can neither restrict nor methylate...expression of T7 RNAP from the genome; does not restrict methylated DNA fhuA2 lacZ::T7 gene1 [lon] ompT...adding the inducer. Growth Media: Changing media is tricky, because there can be a trade-off between growth...about your favorite protein expression tips and tricks! Leave a comment below or browse Addgene’s collection...

  13. COVID-19 Resources

    Type
    Collection
    ...Addgene has partnered with Ginkgo Bioworks to distribute SARS-CoV-2 plasmids. The collection contains:... a for-profit entity, please see our Plasmid Distribution to Industry page. The list of plasmids available...Cathepsin L - a cysteine endosomal protease that triggers proteolysis of SARS-CoV Spike protein, activating...

  14. Deisseroth INTRSECT Collection

    Type
    Collection
    ...TVA-mCherry Cre AND Flp pAAV-nEF-Con/Fon OG Cre AND Flp Triple recombinase-dependent Addgene ID Plasmid Logic ... evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons. Elife. 7. pii: e39786... Sabatini BL. 2019. Distinct Cortical-Thalamic-Striatal Circuits through the Parafascicular Nucleus. Neuron...

  15. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Michael Davidson from Florida State University has contributed their comprehensive collection of ORFs tagged...found below. In addition, the Davidson lab has contributed the corresponding empty backbone with different...

  16. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...plasmids available to scientists in academic and industrial organizations. For more information on ordering... a for-profit entity, please see our Plasmid Distribution to Industry page. These plasmids have not been...

  17. AAV for Neuronal Tracing

    Type
    Collection
    ...retrograde neuronal tracing. Note : Addgene does not distribute rabies virus. AAV Vectors for Monosynaptic Neuronal..., Young JA, Callaway EM. 2007b. Monosynaptic restriction of transsynaptic tracing from single, genetically...

  18. Antibody Plasmid Collection

    Type
    Collection
    ...Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies made from...neuroscience research applications from the NeuroMab/Trimmer Lab Recombinant mAb Collection . Once the plasmids...

  19. mTOR Pathway

    Type
    Collection
    ...mTORC1 integrates these signals to determine the nutritional state of the cell and regulates the switch from...lysosome biogenesis. mTORC2 does not respond to nutrients, but it is activated by growth factors, including...

  20. AAVED

    Type
    Collection
    ...successfully adopt these tools. Thank you for your contributions and your commitment to open science! Please ...meeting preparation section for how to best help contribute to this publication. Citation information for...
Showing: 81 - 100 of 882 results