...                     A post shared by Xi Chen (@seleena78) The lab has officially welcomed the Christmas...                     A post shared by Xi Chen (@seleena78)   Here’s how to enter: Post your picture or...In January, Addgenies will vote on the entries, select up to three winners, and reveal the winning entries...

... PEI transfection calculator for AAV packaging Select the plasmid molar ratio you would like to use for...RepCap OR 3 Transfer Plasmid : 5 pHelper: 2 RepCap Select what type of tissue culture dish you will be performing... transfection in: 15 cm dishes OR CF5 stacks Select how many dishes or stacks you plan to transfect...

...are an important foundation for life on Earth. Selective breeding methods have shaped the plants that we...each for E. coli and Agrobacterium, along with a selectable marker. Left and right T-DNA borders specify ...constitutive Nos promoter, and it can be used to select both transformed Agrobacterium and infected plants...

...sequences to enable both negative and positive selection screening. They and their colleagues including...cancer drug vemurafenib. Enriched libraries for select gene groups The 6 available enriched lentiviral...enough” depends on your experiment and method of selection. Shalem and Sanjana say they kept a high representation...

..., and across species, it enables researchers to select and generate physiologically relevant models. In...of which make it a non-ideal drug. If a drug selectively kills the wildtype line with minimal to no toxicity... and money to make sure quality candidates are selected.      CRISPR aids in multiple stages of the...

...following questions can serve as a guide when selecting a research project. What will the impact be ...students to consider their goal of PhD training when selecting a research topic. The end point of training is...milestones and working hard. Being patient, being selective, and talking to peers and mentors will help elucidate...

.... Avi-Tag plasmid for improved biotinylation selectivity A common strategy to biotinylate proteins involves... cannot be well-controlled. The lack of site selectivity typically diminishes the apparent affinity for...amines are located near the ligand binding site. A selective approach entails the use of the enzyme biotin ...

..., Origins of Replication, Resistance gene and Selection Markers) and one “Transfer Plasmid” game board... on each element to use, they had to place the selected card on the board game. They had 35 minutes to...used to build the construct and explain why they selected a certain target cell population. Who is the winner...

... al.)    Putting MORF to work Screens and selections In its current format, the MORF library is most...Library, the MORF library can be used with other selection and screening mechanisms, although the unique ...Zhang lab validated their Atlas’s TF fate map by selecting a panel of candidate TFs which were predicted ...

...things slowly! It is more important to carefully select first hires who are well-suited, productive, and...themselves out of great projects as ‘impossible' or useless.” How do you run your lab differently or the same...importance of slowly building up your lab and selectively picking the first couple of hires to build a ...

...features such as antibiotic resistance genes, selectable markers, promoters, origins, tags, and certain...location in the plasmid and then a second location to select the sequence between the two locations and send... two separate locations in the linear sequence selects the sequence between the two locations and sends...

...Phage-Assisted Non-Continuous Evolution (PANCE) selection systems to engineer better and faster ABEs (Fig...protein evolution through many rounds of mutations, selection, and replications per day.  The PACE and PANCE...less overall dilution and less stringent phage selection. Figure 2: Phage-assisted evolution of...

...by allowing the user to select for variants that infect the CNS while selecting against those that also... which one applies both positive and negative selective pressures for novel capsids. In a continuation...

...cultured in a special medium called HAT. This medium selects against unfused myeloma cells and antibody-producing...producing the most specific antibodies. Finally, the selected hybridoma is expanded as a clonal population (...experimental results. When you are originally selecting a hybridoma, you can choose one that produces ...

...repair donor plasmid with homology arms and a selection marker. If things work correctly, the second plasmid...antibiotic resistance to neomycin so that you can select the population of cells with the tagged transcription...adapt these vectors for use with their favorite selection cassette. They could also switch from FLAG to ...

...type you select, don’t forget to save a lane for your standards (ladder). You’ll want to select a pre-stained...categories: protein-based and chemical-based. Before selecting one, check your antibody information. Many antibodies...The Basics of Western BlottingAntibodies 101: Selecting the Right AntibodyAntibodies 101: Secondary Antibodies...

...this series described a practical approach to selecting a bright fluorescent protein and a photostable...third post of this series, we will discuss how to select a non-aggregating fluorescent protein. In the jellyfish...of interest before starting your experiments.  Selecting a non-oligomerizing protein A fluorescent protein...

...infected with this virus, and therefore, will be selectable with the antibiotic whose resistance gene is ... encoded in the viral vector. This antibiotic selection can be performed approximately 72 hours after ...–50% of the packaging cells after 1–3 days of selection. A lower percentage of surviving cells may be ...

.... used phage-assisted evolution to generate and select for Cas9 mutants with PAM flexibility. In this ...mutation of the gene of interest. They created a selection phage (SP) to carry catalytically dead SpCas9 ... made to Cas9. It is a pleasant surprise that selecting for one beneficial trait, PAM compatibility, also...

... translucent bodies. This is the ideal stage to select for females because you can be certain that they...Using various markers (more on that below), you can select for progeny with the correct genotype for further...extremely effective. Their Snapdragon tool helps you select primers to synthesize RNA for gene silencing. Additionally...