...ultracentrifugation. The steps are clearly distinguishable thanks to the use of phenol red. Image adapted...

...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-...

...commonly used affinity reagents. Antibodies are Y-shaped proteins, naturally produced by the immune system... These four domains come together to form a “Y” shape, as shown in Figure 1. The two arms of the Y structure...

...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8):783-...

...researchers to share information about the emerging SARS-CoV-2 virus quickly and openly. By sharing results ...you’re ready to write up the paper and share your results, share them as best you can! At the time of writing...rising use of preprints, or scientific manuscripts shared by the authors on a public server independent from...allow authors to avoid many of these issues, by sharing their work publicly and immediately once the manuscript...preprint as evidence of their productivity that can be shared with hiring and review committees, as opposed to...reviewers’ comments, and author responses can be shared directly alongside the preprint while the work ...working manuscript as a preprint too and you can share all the details that you couldn’t fit on your poster...

...who have generously shared their reagents freely with the research community. Sharing is caring We’re thrilled...hooked on sharing because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's...plasmids deposited at Addgene enhance scientific sharing worldwide but a select few are particularly special...honor the creativity, passion, and dedication to sharing epitomized by the creators of these plasmids with...pleased when scientists around the world choose to share their plasmids through our repository since we know...says, “Addgene has transformed the way in which we share plasmids and other molecular tools with the scientific...appreciate our depositor’s dedication to scientific sharing, we wanted to celebrate our depositing PI’s who...

...you want to share your biological materials under! While most plasmids at Addgene are shared under the ...process. Even though this process is essential to sharing plasmids around the globe, it can be a bit of a...of use for a tangible research material that is shared between two parties. There are many different flavors...negotiate individual MTAs every time they wanted to share materials, and the process was time-consuming and..., which is the MTA most plasmids at Addgene are shared under, is well-understood and accepted by the majority...centralized tracking when biological reagents are shared — both at what time and with whom. Tracking these...scientific ecosystem, including understanding how shared materials are used to drive forward new discoveries...

...4,7,9) - are asked why they don’t share, many express concern about sharing negative data they cannot explain...What AAV vector is best for my specific system?  Sharing data and community concerns AAV vectors are complex...particular importance to these efforts is the open sharing of negative data.  Negative AAV data is highly ...scientists who support open science, but do not share their own data - which seems to be the majority ...therefore actively work to remove the stigma around sharing negative data and instead focus on getting this...there?  The Addgene Data Hub is a free resource to share this data and help speed scientific progress, but...if many scientists choose to help each other by sharing their data. So take advantage of it and get your...

... to easily share plasmids with each other. Together, we can transform the antibody sharing landscape as...from Addgene. How Addgene is Reimagining Antibody Sharing Addgene is building our antibody resource, which...antibodies, educational materials, protocols, and shared data, on our values of transparency, reproducibility...community-building. The transparency begins with sharing sequence information for the antibodies in our ...excellent for reproducibility because they do not share hybridomas’ genomic drift or limited material from... toolmakers and tool users. We encourage you to share your recombinant antibodies via Addgene to maximize...accompany our antibody collection.  Addgene is sharing our protocols for recombinant antibody production...

...We’re big fans of all forms of research sharing and with Open Data Day 2020 coming up on March 7th, what... what better way to celebrate open data sharing than with Addgene’s AAV Data Hub! We launched this pilot...using Addgene AAV viral preps to easily find and share useful technical data. These data focus on experimental...injection site from the AAV data hub. How to share your AAV data Do you use Addgene AAV tools and want...ranks of our growing group of Addgene AAV data sharing champions? If you’ve recently used Addgene AAV ...simple steps you can take to input your data for sharing on the site. The entry form has 5 general question...questions and so that we can give you recognition for sharing your data. Materials: What virus and serotype did...

...researchers worldwide. Countless breakthroughs enabled by shared resources. As we step into 2025, these milestones...impact that happens when scientists come together to share their work. The power of community in 2024 In 2024...scientific community's commitment to open science and shared progress. But numbers tell only part of the story...Building tomorrow’s science today Your voices helped shape our journey throughout the year. Through surveys... faster. We’re also building a portal to easily share sequences and characterization data for over 150,000...believe that innovation flourishes when data are shared. The scientific landscape is evolving rapidly, ...innovate with your help and guidance. Every plasmid shared, every collaboration formed, and every breakthrough...

... making it all work – most of the time. #1 Thou shalt find enjoyment in your work If you are happy at ...Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead of life Sometimes, work...and with attention to those around you #4 Thou shalt not bear the burden alone You, alone, are not solely... friends. If there is any guilt, you should be sharing it absolutely equally, which means you only have...get enmeshed in a serious relationship. #5 Thou shalt not sweat the small stuff The house can be messy...yourself; you will make progress over time. #6 Thou shalt throw money at the problem If you can, pay someone...to relax and be with friends and family. #7 Thou shalt create community to help Involve family. If you ...

...you’ll pretty quickly notice the name Vladislav Verkhusha popping up again and again, and for good reason... those tools are great for many applications, Verkhusha and his lab at Albert Einstein College of Medicine... mammalian tissues are nearly transparent. As Verkhusha explained it to me, the transparency window of...the transparency window of mammalian tissues,” Verkhusha said. “We developed near-infrared fluorescent ...deeper.” Adding to the toolbox In the last year, Verkhusha added two far-red light photoactivatable (PA) ...background.” Just last month in Scientific Reports, Verkhusha’s team reported another advance in the application...is because the light becomes very scattered,” Verkhusha explained. Ultrasound wavelengths, on the other...

... believe in openly sharing their plasmids. It’s been a privilege to find and share what stories we can...for our future. And while we’ve been excited to share them with you, we realized our 20th anniversary ...walk through some of our favorite milestones and share some thoughts from the community that makes us… ...open science nonprofit that would facilitate the sharing of plasmids between scientists. The service would...resource. I really appreciate the work you do to make sharing reagents and protocols so easy!” — Nina Jain, Harvard...2012, our repository was rapidly able to support sharing of CRISPR constructs and information across the...lentiviral and AAV vectors, as well as developing and sharing supportive educational resources. By 2017, we had...

...least a shared sense that we’re not alone in struggling with our western blots. Thoughts about sharing Addgene...helping scientists share between labs, and people work hard at Addgene to make plasmid sharing as easy as possible.... The four of us would eat together every day, sharing ideas for the company, but also talking about sports...table and we eat together Harry Potter-style.The shared lunches create a culture of openness and trust ... just as important to take a break from work to share time with the people around us. It makes the quality... every day. Do you have any traditions that you share with your labmates, be they food related or not?...

...environment and hence making it searchable and shareable, but some ELNs provide better support for reproducibility...the integration with protocols.io, the protocol sharing repository. As illustrated in this video the integration...RSpace becomes part of a workflow that enhances the sharing and reproducibility ecosystem protocols.io makes...Zenodo, Open Science Framework, Data Dryad, and Figshare. Any ELN that facilitates reproducibility has ...RSpace has been integrated with two of the five: Figshare and Dataverse, as well as the DSpace repository...integration is described in this video and the Figshare integration in this video.  The ability to link... RSpace using the RSpace datasets deposited in Figshare or Dataverse has access not only to the RSpace...

...believing, as we do, that sharing speeds science.  This year, Chonnettia will be sharing more of her perspective...reflect on our achievements in the last year and share the exciting things to come in the new year. I am...what we accomplished in 2023 and thrilled to be sharing our plans for 2024.  In 2023, Addgene distributed... of innovation and impact made possible by the sharing of tools for research: discovered and developed...and website.  In the coming months, we’ll also share our work in evolving Addgene’s strategic vision ...Addgene, it is my privilege to uphold the values that shape Addgene’s mission and work. In 2024, we will be ... to join me here, and on my LinkedIn page, as I share reflections throughout 2024 on Addgene’s work, mission...

...of Plasmid Sharing: Interested in more statistics from Addgene's 10 years of plasmid sharing? Check out...our US counterparts are still sleeping). Plasmid sharing in Europe: Addgene’s request statistics Since 2004...do for them and how the repository can help them share their materials. The new office in London is dedicated...expression vectors. I also used plasmids made and shared by others to develop an anti-tumour therapy using...researchers are craving, like I was, for a better way to share their plasmids. Now I am on the other side of the...discussing a new scientific discovery or invention, and sharing those ideas with others. This love of interacting... blog post Or look at our "10 Years of Plasmid Sharing" Infographic!  ...

...nice genes…" tshirts, and the lucky winner was Shagun Sharma from the University of Akron, Ohio. Congratulations...researchers who weren’t aware of Addgene’s plasmid sharing services, as well as #happydepositors who stopped...leverage their expertise as we advance our mission of sharing science. I got to check out many of the posters...research and we’d love to help more microbiologists share these valuable resources! Developing the Addgene...about our mission of open science and resource sharing and was also a great place to catch up with former...If you have a great new construct you’d like to share with the scientific community, contact [email protected] from all over the world easily access and share their materials! Resources on the Addgene Blog ...

...Marty LaFleur, who shared information about recent CRISPR deposits from Arlene Sharpe’s lab. X-Chime and...ligands. Illuminating Choices Paul Heisig, who shared his flow cytometry expertise in three posts. An...and Beyond Surface Labeling Ashlyn Lemmen, who shared her flow cytometry expertise in two posts. Flow...Flow Cytometry Readouts Toj, a summer intern who shared their experience working at Addgene. An Addgene...collection. Embracing Serendipity Nicolas Mathis, who shared information on his open software that predicts ...and Michael Biddle, of Only Good Antibodies, who shared their antibody validation expertise. Antibody ...