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  1. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ... the lab. GFP is used in research across a vast array of biological disciplines and scientists employ ...maturation, and fluorescence. Biosensors: A wide array of GFP-based fluorescent biosensors has been designed...

  2. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...for the indicated ssAAV5s. Figure adapted from Serrano et al. 2023 under a CC-BY-NC-ND license.  Integrating...  Find microglia-targeting AAV plasmids here! Serrano, C., et al. (2023). Simple and Highly Specific ...

  3. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ... Rhodopseudomonas spheroides, Salmonella spp., Serratia marcescens, Streptomyces lividans, Synechococcus...Salmonella enteritidis, S. typhimurium, S. ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas...

  5. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ...experiment. Here, we will introduce you to the wide array of Cas9s (many available through Addgene’s repository...VP64 along with other SAM factors, and the Suntag array of factors, all targeted to the promoter region....

  6. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...and portability. This method can be used for an array of applications including clinical diagnostics (...detection of nucleic acids in a mixed population for an array of molecular and clinical diagnostic applications...

  7. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F (2013) Multiplex Genome Engineering...Garneau JE, Dupuis M-È, Villion M, Romero DA, Barrangou R, Boyaval P, Fremaux C, Horvath P, Magadán AH...

  8. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...plasmid Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae Chloramphenicol yes, cut Marraffini 44251 pgRNA-bacteria...

  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...commonly used today in molecular biology for an array of different techniques and protocols. In this post... Thermo Fisher Scientific's guide. There are an array of other molecular biology applications for biotin...

  11. Cre-lox system

    Type
    Collection
    ...material will be rearranged. The schematic below shows the three types of rearrangements: inversion, deletion...Cre-Lox system and how it can be used for genetic rearrangements and recombination. Use Addgene's curated tables...

  12. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...identifies a p value cut off. This method entails arranging all calculated p values from smallest to largest...): 126-130 (2012). 10.1097/SMJ.0b013e3182498ad5 Curran-Everett, Douglas. Explorations in statistics: statistical...

  13. Rett Syndrome

    Type
    Collection
    ...developmental transcriptome information, prenatal LMD microarray data, ISH image data, and anatomic reference ..., 944–950. PMID: 21154482 Neul et al. 2019. The array of clinical phenotypes of males with mutations in...

  19. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    ..., in the labs of Chase L. Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing...
Showing: 81 - 100 of 224 results