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  1. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...Zinc Finger Arrays Zinc Finger Consortium: Zinc Finger Arrays Engineered Zinc Finger Arrays for Endogenous...zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs targeted to a...Peterson, and Joanna Yeh are engineering zinc finger arrays targeted to various zebrafish genes. The table...ZFN expression vectors, DNA encoding zinc finger arrays should be cloned into plasmids also available through...more details). IMPORTANT NOTE: All zinc finger arrays listed show transcriptional activation activity...ZFNs in zebrafish. Target Gene Name Zinc Finger Arrays Engineering Method Full Target Site (5' to 3') ...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Elisa Izaurralde 147087 pLNHA-C1-HseIF4G_O EIF4G HA CMV Parkinson's Elisa Izaurralde 147091 pT7-...Parkinson's Elisa Izaurralde 148112 pcDNA3.1-MS2-HA-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148113 pcDNA3.1...proteinopathy, ALS Elisa Izaurralde 148138 pT7-V5-SBP-C1-HshnRNPA1_Z HNRNPA1 CMV ALS Elisa Izaurralde 148139 pT7-V5...ALS Elisa Izaurralde 148154 pnEA-NvM-DmeIF4G_578-650-GB1_Z EIF4G Parkinson's Elisa Izaurralde 148183 pnEA-NvG-DmeIF4G...'s Elisa Izaurralde 148504 pnEA-NpM-DmeIF4G_578-675-GB1_AD EIF4G Parkinson's Elisa Izaurralde 148505 pnEA-NpM-DmeIF4G...Parkinson's Elisa Izaurralde 148506 pnEA-NpM-DmeIF4G_601-660-GB1_AD EIF4G1 Parkinson's Elisa Izaurralde 148507 pnEA-NpM-DmeIF4G...'s Elisa Izaurralde 148508 pnEA-NpM-DmeIF4G_617-634-GB1_AD EIF4G Parkinson's Elisa Izaurralde 148509 pnEA-NpM-DmeIF4G...

  3. TALEN Guide

    Type
    Collection
    ...components of the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays have been... the final array is composed of 18 TAL effector repeats. The kit can be used to make arrays from 12-31...nucleotide triplets. With some trial and error, ZF arrays are able to recognize their targets with fairly... fused to a nuclease or activation domain, a ZF array is an effective targeting mechanism for molecular...and interactions between zinc fingers within an array can reduce their specificity. Two years ago, groups...importance of context for each TAL effector within an array, but early studies suggest the likelihood of context...negative effect on specificity is on par with ZF arrays, if not slightly lower. Figure 2: Simplified representation...

  4. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...into contiguous stretches of DNA known as CRISPR arrays. Separate Cas proteins then express and process...CRISPR system. Within the bacterial genome, a CRISPR array contains many unique protospacer sequences that ...short palindromic repeat sequences. (1) The CRISPR array is transcribed to make the pre-CRISPR RNA (pre-crRNA...cerevisiae and S. pombe ) Zebrafish Xenopus References Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P,..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. 2013. Multiplex genome engineering...Alkhnbashi OS, Costa F, Shah SA, Saunders SJ, Barrangou R, Brouns SJ, Charpentier E, Haft DH, Horvath ...gene editing by CRISPR-Cpf1 using a single crRNA array. Nat Biotechnol . 35(1):31-34. PMID: 27918548...

  5. mTOR Pathway

    Type
    Collection
    ...PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD Ras-related GTP binding Raptor Also known...Loss of the tumor suppressor p53 also promotes aberrant mTORC1 activity, and multiple familial cancers...

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ...system using two cassettes to express the CRISPR RNA array and tracrRNA separately (Cong et al ., Science 2013... SpCas9 (or SpCas9n, D10A nickase) + CRISPR RNA array + tracrRNA: This plasmid contains three expression... indicated below) can be cloned into the CRISPR array. The oligos are designed based on the target site..., Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science . 2013 Jan 3. DOI: 10.1126...V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, Marraffini LA, Bao G, Zhang F. Nat Biotechnol. 2013 Sep;... 10.1016/j.cell.2013.08.021. Epub 2013 Aug 29. Erratum in: Cell. 2013 Oct 10;155(2):479-80. PubMed . Genome...

  7. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Chloramphenicol Marraffini pCas9 42876 Bacteria BsaI yes, cut S. pyogenes Chloramphenicol Marraffini pgRNA-bacteria...interfere S. pyogenes Marraffini pWJ66 46570 Bacteria BsaI yes, activate S. pyogenes Marraffini pWJ68 46571 Bacteria...Bacteria BsaI yes, activate S. pyogenes Marraffini pSimpleII-U6-tracr-U6-BsmBI-NLS-NmCas9-HA-NLS(s) 47868...rapid modular assembly of natural type II-A CRISPR arrays capable of simultaneously repressing multiple target...

  8. Bacterial Expression Systems

    Type
    Collection
    ...Technique PI Purpose pCas9 42876 CRISPR Luciano Marraffini Bacterial expression of Cas9 nuclease gRNA. For...Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence for RNA-binding protein,...Philippe Cluzel RNA localization: Contains a 2x array of the binding sequence for RNA-binding protein ...interest. Plasmid ID Promoter Inducer(s) PI Purpose Murray Lab pBEST plasmids Various Plasmids pBAD, OR2-OR1...Arabinose, Anhydrotetracycline, lactose, IPTG Richard Murray Plasmids based off of pBESTluc from Promega. Contain...

  10. Viral Vectors

    Type
    Collection
    ...to harbor a wide array of viral genomes, enabling researchers to direct a wide array of genetic functions...that can be used to engineer viruses for a wide array of research purposes. Ready-to-use viral preparations...that can be used to engineer viruses for a wide array of research purposes. Which Type of Virus Should...

  11. TALEN Plasmids and Kits

    Type
    Collection
    ...Binding specificity is determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors...efficiently assemble TALEN constructs with custom repeat arrays. TALEN Kits TALEN Kit Description Depositing PI...Yamamoto This accessory pack contains modified pFUS array vectors, destination vectors and a reporter vector...cloning DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast, or FLASH ...

  12. CRISPR Guide

    Type
    Collection
    ...expressing Cas9 and a gRNA will result in a diverse array of mutations (see: Validate genetic modification... a given genome. Some more recent libraries use arrays of multiple gRNAs to target paralogs or lncRNAs...R., Habib, N., Hsu, P. D., Wu, X., Jiang, W., Marraffini, L. A., & Zhang, F. (2013). Multiplex genome ...Fine, E. J., Wu, X., Shalem, O., Cradick, T. J., Marraffini, L. A., Bao, G., & Zhang, F. (2013). DNA targeting...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature , 516 (... S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, ...CRISPR Journal . 2 (6), 376–394. PMID: 31742433 Durrant, M. G., Fanton, A., Tycko, J., Hinks, M., Chandrasekaran...

  13. Zinc Finger Consortium Reagents

    Type
    Collection
    ...finger arrays via OPEN method Modular Assembly Engineering Reagents - Engineer zinc finger arrays via Modular... Finger Genome Engineering Reagents Zinc Finger Arrays for Zebrafish Genes - Browse pre-constructed zinc...

  14. TALEN Expression Vectors

    Type
    Collection
    ...Mammalian expression vectors for TAL effector repeat arrays assembled using the REAL, REAL-Fast or FLASH methods...which DNA fragments encoding TAL effector repeat arrays (assembled using the REAL, REAL-Fast or FLASH methods...three of these methods yield TAL effector repeat arrays with identical DNA and amino acid sequences (i....

  15. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...Institute Pasteur has deposited a collection of CRISPR arrays to modulate the expression levels of two targets...DNA-binding protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is ... can be controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with...

  16. TALEN Engineering

    Type
    Collection
    ...three platforms for engineering TAL effector repeat arrays of any desired length: the REAL (Restriction Enzyme...of these platforms assemble TAL effector repeat arrays on a single architectural framework and the DNAs... are identical in sequence. TAL effector repeat arrays produced on this framework has yielded TALENs which...

  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...targeted GCaMPer Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...sensor (cytosolic) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...oxidation (roGFP2-Orp1) Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular... 2017 Nov 10;8(1):1412. Justin Taraska , David Perrais pH Sensor for intracellular chloride and pH (ClopHensor...or mitochondria Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular...

  18. CRISPR References and Information

    Type
    Collection
    ...PDF 165.1 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF 125.6 KB Marraffini Bacteria: pCRISPR...pSL1180polyUBdsRED PDF 583.3 KB Zhang gRNA cloning CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9...

  19. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...209552 Knockout Human Doench 3rd 1, 2, or 4 49,766 arrays Broad GPP kinome Brunello 75314, 75315 (1 plasmid...Library 130630 Knockout Human Draetta 3rd 3-4 17,032 arrays Mouse Cardiac Transcriptional Regulators CRISPR...Library 123361 Knockout Mouse Chen 3rd 4 11,934 arrays Mouse SLC Knockout Library 141436 Knockout Mouse...

  20. Individual REAL TALE Repeat Plasmids

    Type
    Collection
    ...and robust protocol for constructing TALE repeat arrays of any desired sequence, referred to as the REAL...restriction sites used for ligation into longer arrays. These 28 plasmids are available individually or...
Showing: 1 - 20 of 36 results