...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). ...Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination...

...selection markers (for use in other cell types, e.g. eukaryotic cells). pBACKBONE-XXXXX Tip: For a catalog of...

...of inducible promoters capable of controlling eukaryotic gene expression. Several endogenous promoters...Scientists began to pursue a non-endogenous system for eukaryotes. At the time, bacterial systems from E. coli ...

... the researchers led by Dr. Jorge Ruas of the Karolinska Institutet, the discovery really wasn’t what ...

...avenue of modifying and manipulating endogenous eukaryotic RNA.” Endless possibilities with PUF proteins...

...bacterial species, but newer versions engineered for eukaryotic cells are available and further improvements ...Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is recruited to target...25122746 Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. ... Science , 339 (6121), 823–826. PMID: 23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,... Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp, P. A., & Zhang, .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby..., Herreno-Pachón, A., Benincore-Flórez, E., Karunathilaka, A., & Tomatsu, S. (2024). Current strategies...

...user. Read more about it on the Addgene blog . Eukaryotic Pathogen CRISPR guide RNA/DNA Design Tool (EuPaGDT... gRNA design tool with extensive selection of eukaryotic pathogen genomes (200+) that can predict gRNA...

...introduction of nucleic acids into bacteria or eukaryotic cells is a common technique in molecular biology...

...post-translational modifications added to proteins in other eukaryotes better than E. coli. Plasmids designed for these...

... References Geyer, Roland, Jenna R. Jambeck, and Kara Lavender Law. "Production, use, and fate of all ...

... Jane, Jason, Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly, Kory, Kris, Leila, Lianna, ...

...your biosensor Before expressing a biosensor in eukaryotic cells, think about the best place for the biosensor...

... your imaging time to get enough signal. This workaround risks cellular phototoxicity and eliminates the...

...bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature, plasmids...

...plain sight: what remains to be discovered in the eukaryotic proteome? Open Biol. (2019) 9:180241. DOI: https...

...translocation Bacteriostatic 5-25 ug/mL in EtOH *In prokaryotes.     **Dissolve in dH2O and sterile filter unless...

...better for PCR and surveyor. I used DNA polymerase Takara Ex Taq ™ DNA Polymerase for my genomic PCR, but...successfully used Herc II or Kapa Hifi in PCR. Maybe this Takara enzyme is not very robust in this case for EMX1...

...State University, Indiana University, and the Karolinska Institute developed mNeonGreen via 21 substitutions...

... and former Associate Director of Viral Vectors Karen Guerin for the journal Molecular Therapy. Vandenberge...

...We have also packaged hChR2(H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon...