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  1. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... Depositor Collections Bikard Lab - CRISPR Repression Collection Bikard Lab CRISPR Repression Plasmids...From the Bikard Lab, CRISPR plasmid collection for modulating expression levels of two targets independently...Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard Lab at the Institute Pasteur has deposited a collection...article: Vigouroux, A., Oldewurtel, E., Cui, L., Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s...

  2. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Optogenetics Karl Deisseroth AV-1-26966P 26966-AAV1 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26968P 26968-AAV1 pAAV-Ef1a-DIO ChETA-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26971P 26971-AAV1 pAAV-CaMKIIa-eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-1-26975 26975-AAV1 pAAV-CaMKIIa-hChR2(H134R)-mCherry Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26966P 26966-AAV5 pAAV-Ef1a-DIO eNpHR 3.0-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26969P 26969-AAV5 pAAV-CaMKIIa-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...Optogenetics Karl Deisseroth AV-5-26973P 26973-AAV5 pAAV-hSyn-hChR2(H134R)-EYFP Optogenetics Karl Deisseroth...

  3. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...An overview of CRISPR's development from a prokaryotic immune system to a valuable genome engineering...revolution shows no signs of slowing down. This prokaryotic immune system is especially amenable to genome...offering flexibility and easy multiplexing/scaling. Prokaryotes have long utilized CRISPR as a powerful defensive...elucidated until 2007. Scientists hypothesized that prokaryotes used CRISPR as part of an adaptive immune system...PAM sequences and number/types of Cas proteins. Makarova et al. ’s classification defines 5 types and 16...system seems like a winning strategy to ensure prokaryotic survival, but it is not invincible. Bondy-Denomy...provides acquired resistance against viruses in prokaryotes. Science . 315(5819):1709-12. PMID: 17379808 ...

  4. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...OPTN His T7 ALS Jon Ashwell 23376 pDONR223-PRKAR1B PRKAR1B Dementia and/or parkinsonism William Hahn 23452...Parkinson's, FTD Karen Ashe 46905 pRK5-EGFP-Tau AP MAPT GFP CMV Parkinson's, FTD Karen Ashe 46906 pRK5-...Parkinson's, FTD Karen Ashe 46907 pRK5-EGFP-Tau E14 MAPT GFP CMV Parkinson's, FTD Karen Ashe 46908 pRK5...Parkinson's, FTD Karen Ashe 46909 pRK5-EGFP-Tau E14 P301L MAPT GFP CMV Parkinson's, FTD Karen Ashe 46924 YFP-Parkin...-Spatz disease David Root 76838 PRKAR1B gRNA (BRDN0001146606) PRKAR1B hU6 Dementia and/or parkinsonism...parkinsonism David Root 76839 PRKAR1B gRNA (BRDN0001144765) PRKAR1B hU6 Dementia and/or parkinsonism David Root ...Root 76840 PRKAR1B gRNA (BRDN0001147089) PRKAR1B hU6 Dementia and/or parkinsonism David Root 76898 PRKCG ...

  5. CRISPR Guide

    Type
    Collection
    ...bacterial species, but newer versions engineered for eukaryotic cells are available and further improvements ...Cascade-Cas3 system has been co-opted for use in eukaryotic cells . The Cascade complex is recruited to target...25122746 Kleinstiver, B. P., Prew, M. S., Tsai, S. Q., Topkar, V. V., Nguyen, N. T., Zheng, Z., Gonzales, A. ... Science , 339 (6121), 823–826. PMID: 23287722 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,... Kriz, A. J., Zetsche, B., Shalem, O., Wu, X., Makarova, K. S., Koonin, E. V., Sharp, P. A., & Zhang, .... J., Hussmann, J. A., Yan, J., Knipping, F., Ravisankar, P., Chen, P., Chen, C., Nelson, J. W., Newby..., Herreno-Pachón, A., Benincore-Flórez, E., Karunathilaka, A., & Tomatsu, S. (2024). Current strategies...

  6. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ... 33,170 Bikard Lab E. coli Genome-wide Inhibition Library v1 115927 Inhibition E. coli Bikard N/A 19 92,919...92,919 Bikard Lab EcoWG1: E. coli Genome-wide Inhibition Library v2 131625 Inhibition E. coli Bikard N/A ...

  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ... CKR-1, CMKAR1, CXCR1, IL8R1, IL8RBA IL8RB interleukin 8 receptor, beta CD182, CDw128b, CMKAR2, CXCR2,... (C-X-C motif) receptor 3 CD182, CD183, CKR-L2, CMKAR3, GPR9, IP10-R, Mig-R, MigR CXCR4 chemokine (C-X-C... protein tyrosine kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I ULBP2...Smith B, Schaefer H, Chen J, Hu Z, Zalocusky KA, Shankar RD, Shen-Orr SS, Thomson E, Wiser J, Butte AJ. ...

  8. Retrograde AAV viral preps

    Type
    Collection
    ... viral service. Retrograde construct from Alla Karpova at Janelia Research Institute....preparations in a retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector... these viral preparations was performed by the Karpova lab. Results for our quality control experiments...

  9. Cre-lox system

    Type
    Collection
    ...mCherry and Cre Lentiviral Luikart 66691 FUG-T2A-cre GFP and Cre Lentiviral Luikart 66692 pRubiC-T2A-cre mCherry...Retroviral Luikart 66693 pRubiG-T2A-cre GFP and Cre expression in newborn neurons Retroviral Luikart 67503 ...

  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...PXL 75349 Mammalian U6 yes, activate S. pyogenes Luikart pRubiG-T2A-Cas9 75348 Mammalian/Retroviral from...from pXL yes, activate S. pyogenes EGFP Luikart pRubiC-T2A-Cas9 75347 Mammalian/Retroviral from pXL yes,...yes, activate S. pyogenes mCherry Luikart pDECKO_mCherry 78534 Mammalian hU6 none S. pyogenes Puro, mCherry...

  11. mTOR Pathway

    Type
    Collection
    ...provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein...protein 1 Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR...

  12. AAVED

    Type
    Collection
    ... School Dr. Melina Fan, Dr. Susanna Bachle, Dr. Karen Guerin, Dr. Leila Haery, Dr. Meghan Rego, Addgene...Campus Kenton Woodard Penn Vector Core Melina Fan, Karen Guerin, and Leila Haery from Addgene will also be...

  13. Viral Production

    Type
    Collection
    ...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). ...Mycoplasma The 293T cell line was obtained from Takara, and is routinely tested for mycoplasma contamination...

  14. Tetracycline Inducible Expression

    Type
    Collection
    ...of inducible promoters capable of controlling eukaryotic gene expression. Several endogenous promoters...Scientists began to pursue a non-endogenous system for eukaryotes. At the time, bacterial systems from E. coli ...

  15. CRISPR References and Information

    Type
    Collection
    ...user. Read more about it on the Addgene blog . Eukaryotic Pathogen CRISPR guide RNA/DNA Design Tool (EuPaGDT... gRNA design tool with extensive selection of eukaryotic pathogen genomes (200+) that can predict gRNA...

  16. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...better for PCR and surveyor. I used DNA polymerase Takara Ex Taq ™ DNA Polymerase for my genomic PCR, but...successfully used Herc II or Kapa Hifi in PCR. Maybe this Takara enzyme is not very robust in this case for EMX1...

  17. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...please try viewing on YouTube . Abstract The prokaryotic clustered regularly interspaced short palindromic...9 system may be re-purposed for site-specific eukaryotic genome engineering. CRISPR/Cas9 is an inexpensive...

  18. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase. Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation...luciferase from broad host range plasmid Attila Karsi 105621 Lenti-luciferase-P2A-Neo Firefly EF1α Lentiviral...

  19. Brain Initiative Collection

    Type
    Collection
    ... PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination...

  20. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression...Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Mitochondrial...
Showing: 1 - 20 of 36 results