We narrowed to 749 results for: Dos

...customer place an Addgene order using a PO number? Why do I have to order directly from your website? Many ...sent to the contact email addresses provided. How do I place an order using a PO number? Placing an order... PO, learn more about how to create a quote. How do I pay the invoice? After your Addgene order ships...this article, check out our Help Center article How do I pay by purchase order (PO) number? You can find...

...Yeast ID Plasmid Gene/Insert Promoter PI Publication Do you have suggestions for other plasmids that should...

...Type Selectable Marker PI Publication Back to Top Do you have suggestions for other plasmids that should...

...you in building a grant career. Do you have a passion for the arts? Do you find satisfaction in helping...Chat, and then our grant training and mentoring. How do I become a grant professional? Employment opportunities...healthcare facilities and with private businesses. How do you get the expertise needed to enter the grants ...helping your local food shelf raise money? Do you have experience with research grants? Are you interested...find work and target your professional development. Do you want to work in a particular area, e.g. education... childhood development, or community development? Do you want to focus on a part of the grant development...

...Wood of Duke University Medical Center. “While we do not yet have a comprehensive understanding of how... become resistant, an emerging theme is that they do so by activating signaling pathways controlling properties...contexts, so he assembled a plasmid kit designed to do exactly that. As described in the journal Science... world who are interested to use our libraries to do things - sometimes drug screens and sometimes totally...

...Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should...

...Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should...

...Blogs CasPEDIA Content last reviewed: 9 December 2025 Do you have suggestions for other plasmids that should...

...are trying to achieve: Do you want to permanently knock out or knock-in a gene? Do you want to activate ...Are you trying to create a single point mutation? Do you want to create a fusion with a reporter protein...appropriate expression system and delivery method: Do you need stable integration or is transient expression...sufficient? Which cell types will you be editing? Do you want to deliver the components as DNA, or would...surprised at the number of options available - how do you choose between them? One of the first steps is...

... what position do you want your gene to be in relation to the rest of the genes? And do you want it to...the Modular Cloning (MoClo) system and what it can do for you, and you’ve chosen one of the MoClo kits ...coding sequence, a terminator, or other plasmid part? Do you want to fuse your protein to any tags or fluorescent...your MoClo assemblies in silico before you start doing your cloning. Most sequence analysis software includes...preventing your overhangs from aligning properly. Do this sequentially for your Level 0, Level 1, and ...viability. When handling your MoClo plate, always do so in cold conditions and preferably on dry ice. ...

...have any questions, comments or suggestions, please do not hesitate to email me at [email protected] need a Nature/Cell/Science paper. You do need multiple first/corresponding author publications...exciting idea in a swamp of unnecessary details. You DO NOT need to present a detailed review of your field...field or the finer points of your techniques. You DO need to be prepared to know the literature of your...proteins is essential for human health. Failure to do so is implicated in several human diseases. Proper...bring in grants. What kind of colleague will you be? Do you understand what it takes to be successful in ...a Department's/Institution's success is the task. Do your homework. Learn what your future potential colleagues...

...Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids that should...

...content, website, Data Hub, and everything else we do! The goal of Addgene’s educational content Addgene...their favorite science topics. What better space to do that than in the Addgene blog?  Fig. 1: One...editing process and final check before publication.   Do research! Nearly everything we write entails a primary...papers or image sharing repositories. Then we format, do a final review to catch those pesky typos, and hit...advance the scientific community, and we can only do that with your help.     Additional Resources on...

...in the science community, we’ve been motivated to do anything and everything we can to help find a cure...by approaching it from a new direction, we hope to do it much faster. Years of science, done in weeks ...form the QBI Coronavirus Research Group (QCRG) to do just this.  In under a month’s time, we’ve published...are starting these experiments, there's so much to do that we can use the whole science community's help...  And, if you’d like to keep up with what we are doing at QBI or the QCRG to continue our work toward a...

...replicating previous research findings so difficult to do?  There are four reasons that research can be difficult..., QRPs, and small samples, then when replications do not come out the same as an original research finding...difference for the robustness of the findings.   How do we make research more reproducible? Combat unclear...of other researchers (or your own from years ago!) do you really appreciate how few details are provided...while the research is ongoing, but all registries do require that research plans become public eventually...

...the Addgene Blog AAV Titers: Where do they come from and what do they mean? AAV Vector Quality Control...AAV Protocol (Step 10) for instructions on how to do this. Concentrating your virus will allow you to ...titer when looking for effective gene expression. How do you achieve high titers in the first place? Some ...surrounding solution to ensure excess viral particles do not remain adhered to the membrane. Be careful when...

... is done within publication years. What do we find once we do this adjustment? That papers with plasmids...story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits accumulate... with Addgene plasmids get more citations So what do we find? Lots more citations for the papers with ...analysis cannot rule out all other explanations, we do think that it lends weight to the argument that Addgene...

...scope can lead to long meetings spent on details that do not yet need to be decided. Figure out what you need...level of meeting you need. Project Meetings: You do not have to include every person who could potentially...notes on the agenda points. At Addgene, we like to do this live on the screen using a Google Doc. Action...instead of reading the whole thing together. How long do you actually need? Just like liquids, meetings tend...with just one or two relevant people. How often do you need to meet? Use trial and error to determine...

... and has a To-Do list long enough that there is no hope of accomplishing it all. How do we encourage people...Days, teams at Addgene will forgo their regular To-Do lists (other than emergency items) and focus on a...large table to eat with my Addgene family every day. Do you have any traditions that you share with your ...

...becomes much more complex. “How do I reach out to potential mentors?” “Where do I start with picking universities...applying to a school that requires its students to do rotations in different labs before selecting an advisor...is the right fit. Reaching out to PIs also means doing plenty of mini-interviews; these professors will...universities offer free practice sessions for students doing virtual interviews, so check if yours does and then...things like sickness or scheduling issues can and do interfere with those in-person plans. Virtual options...