We narrowed to 769 results for: Dos
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TypeCollection...comes from. Then consider the following questions: Do you need polyclonal or monoclonal antibodies? Will...direct or indirect detection method? If indirect, do you already have secondary antibodies available in...have identical features and epitopes, to ensure you do not validate the same antibody multiple times. You...antibodies will also have a source species. What conjugate do you want to use? Most secondary antibodies will be...buy small aliquots of many different antibodies and do batch validation up front rather than risk delaying...
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Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog Post...dicots, not grasses and wheat, but we do work in wheat here. We do lots of work on wheat rusts and we are...made the most sense to us to enable other people to do that too. We don’t have the capacity to send tools...have comparable expression levels to things people do use a lot. The problem in plant science is there ...good laboratory workhorse. Tips & tricks Addgene: Do you have any particular tips for those looking to...expression construct on the same piece of DNA. We do a lot of protein-protein interaction work and we’...is an analysis of women in faculty, how they are doing. They look at structure, impediments, the representation...work in plant science. I think their chances of doing something amazing will be equally high as for those... -
Finding Your Perfect Job After University
TypeBlog Post...parasitology but another major factor held me back; I do not have a second language. During the recession ...these positions. I had to reassess what I wanted to do and so I applied for a role in Industry. I wanted...wanted to get "a real job" and roles in Industry do tend to pay better. I worked in a Microbiology testing...and spreadsheets. It was great to be in the lab doing experiments without the constant fear of failure...focus always seemed more on getting results than doing good science. Another problem was that the work ...monotonous very quickly; as soon as I learned how to do the different tests and became efficient, I then ... had to look at what type of work I would like to do. I had taken advanced virology classes in California... -
Networking Follow-up: I Met a Person. Now What?
TypeBlog Post...relationships with people you care about knowing. How do you follow up with new connections and build the ... note about where you met and what you discussed. Do this in a timely manner (within a few days or a week... Here are a few things you probably don’t want to do: Don’t connect via LinkedIn with strangers. It’s... that don’t respond; focus on the nice people who do respond. Don’t call or email multiple times if you...They now message me frequently and ask me how I’m doing with nothing else to talk about. I know absolutely...worth connecting with people you don’t like or who do not show interest in you. Network with the people... -
An Addgene Co-op: The Intersection of Fun, Friendship, and Work
TypeBlog Post...collection. While most days I was up to my neck in to-dos, I also found time to go out to sushi restaurants... building strong connections with coworkers and doing something meaningful with my career. I can’t ...as much of her focus is on the scientific work we do while mine is mainly within content and business.... who are interested in understanding the work you do, in addition to being genuinely interested in knowing...safety net and trampoline all at once. I was able to do something that was challenging while helping us share...media spreadsheets every day of my co-op. I had to do research on loads of different best practices, create... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...contracting life where you do a little bit of work for one client and then switch to doing something else. You...always loved science, but didn’t know what she would do if she wasn’t at the bench. “I was literally sitting...with a postdoc, and I’m like, “Oh my god, what can I do?! This is a mistake! Why did I even come [to grad...’s a science writer?” Learning about SciComm by doing SciComm Writing had always been a strength of Susan...s something that good storytellers in science can do.” Susan’s number one piece of advice for getting...on things.” Susan personally found this easier to do while still in school since opportunities, such as... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...improve their current job situation. What can a coach do that is different from an adviser, boss or mentor...have seen, without exception, that this helps people do a better job at reaching their goals. Check out ...aware of the benefits of coaching relationships and do not know where to look for a coach. Many (most?) ...will cost a fortune for a successful outcome. How do I find a coach? Start with referrals. Are there scientists...what your goals are for the coaching relationship. Do you want to change jobs? Grow your professional presence...need to choose well. What are their qualifications? Do they have certifications? How can they demonstrate...and Anthony Collmann of White Consulting frequently do sessions by Skype or phone. Samantha Sutton is another... -
Career Insights: Technical Support Specialist
TypeBlog Post... issue, you will do well in a technical support role. What general characteristics do jobs in technical...Additional Resources on the Addgene Blog Read What Do I Do Now? Academic v. Non-Academic Career Decisions ...results, answer loads of questions, and I also had to do some troubleshooting as advised by the technical ...that rather unpleasant experience I thought: I could do this job so much better. Driven by that, I successfully...different from those specified on its data sheet?” or “Do you have additional data regarding the specificity... Outreach Scientist at Addgene. These days he's doing a lot of science communication, and is particularly... -
An Inside Look into Addgene's Dev Team
TypeBlog Post...supposed to be doing. When this happens, we discuss and agree on a common name. While we do our best to ... another point of collaboration for us. I enjoy doing code reviews because it’s like a little peek into...and fixing bugs: we take time during each sprint to do some maintenance work on our code. One way to look...is tidying up your home. You could choose to only do the dishes once a month, resulting in a dangerous...dangerous mountain of dirty dishes or you could choose to do the dishes after every meal and be reassured that...total of two years, from planning to completion, to do this upgrade while continuing to add new features...Conclusion While none of us are life science majors, we do our best to understand the science. By understanding... -
Lab to Office Culture Shock
TypeBlog Post...tools to help you do your job as well as possible. It’s in their best interest to do so, sure, but it ... something to do and there is no thumb twiddling. But you like the downtime, you say? Do you know what... anymore. Being a scientist is not about what you do--it’s about how you think. I have not worked at the... how much of that time you’re actually spending doing work. Yes, you may legitimately need to be in the...the opportunity to learn their “language”, and in doing so you may discover that your career options are...the meetings you’re attending are a waste of time, do what you can to fix them, and take a look at these... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog Post...company culture! Addgene is the best place to work, we do SO many fun things!!” Okay - honestly, maybe not...thoroughly. It’s kind of a chicken and egg situation- do we work so hard so we have time to play later? Or...how everyone is kind and collaborative and all we do is have fun all day long, whistling while we work...included) that plan big company events and fun things to do throughout the month. Although we are busy with many...tough to come up with new and interesting things to do, feel free to crowdsource! 2. Free food generally...dangle the carrot (or more popularly- cookies). 3. Do something outside of your ordinary environment Team...your office space too Balance point number 3 with doing fun things within your office. This way people don... -
All Antibodies
TypeCollection...Reactivity Recommended Applications PI Return to Top Do you have suggestions for other antibodies that should... -
Plasmid Collections
TypeCollection...Rett Syndrome Plasmid Collection and Resource Center Do you have suggestions for other plasmids that should... -
Optogenetics Plasmid Collection
TypeCollection...Vector Type Promoter PI Publication Return to top Do you have suggestions for other plasmids that should... -
CRISPR Plasmids - Double-Strand Break (Cut)
TypeCollection.../Insert Promoter Selectable Marker PI Publication Do you have suggestions for other plasmids that should... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection...PI Publication Last reviewed on: January 30, 2025 Do you have suggestions for other plasmids that should... -
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection...PI Publication Last reviewed on: January 30, 2025 Do you have suggestions for other plasmids that should... -
Fluorescent Protein Guide: In Vivo Imaging
TypeCollection...PAiRFP2 692/719 (after photoactivation) 3.0 pPAiRFP2-N1 Do you have suggestions for other plasmids that should... -
Viral Prep Service for Plasmids and Pooled Libraries
TypeCollection...make your own preps. Recipient Instructions What to do when you receive your viral vector from Addgene, ... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...reading email). My advice is always "don't do it unless you can do it well" – even the smallest program. I... personal rule is that I do not let the panelists introduce themselves. I do a 1-2 minute introduction... with a few questions. Who is out there? What do they do? Find out their areas of science or expertise...bios were likely part of the publicity. Good panels do not waste time on long introductions. The moderator...