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Showing: 81 - 100 of 195 results
  1. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...mL conical tubes Automated cell counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm ... in a 500 mL vented flask.Incubate in a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm....
  2. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...cre recombinase (either from a mouse line or viral co-injection), the opsin is inverted into the correct...opsin expression, extent of viral spread, and with co-localization immunofluorescence, cell-type specificity...
  3. Brain Initiative Collection

    Type
    Collection
    ...112006-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 For co-expression of GCaMP6s with mKate2 Lin Tian 112007...112007-AAV9 pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 For co-expression of GCaMP6s with mRuby3 Lin Tian 112010-AAV5...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. Jonathan...-hSyn-DIO-jGCaMP8s-P2A-ChrimsonR-ST Cre dependent co-expression of jGCaMP8s and soma-targeted ChrimsonR...
  4. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...2xNLS) fragments facilitates target DNA cleavage upon co-expression and spontaneous self-assembly. In an effort... in response to blue light, which will ultimately co-localize the VP64 transactivator with a dCas9 that...well as other photoactivatable proteins like PAGFP. Co-expression of LOC8R-PSmOrange and LOC8R-PAGFP provides...purify your POI, to perform pull down experiments like co-IPs and ChIP, and more - all without the need to ...specific genomic locus. When fused to the sgRNA and co-expressed with dCas9 – the dCas9, sgRNA/RNA cargo...
  5. Rett Syndrome

    Type
    Collection
    ...ability to bind to methylated DNA and recruit known co-repressors or other transcriptional modifiers suggests...D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and...
Showing: 81 - 100 of 195 results