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  1. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...generating iPSCs by plasmid transfection where 2 of 11 clones tested had plasmid integration. Another challenge...Nonstructural C Protein and Endogenous MicroRNAs." Plos One 11, no. 10 (2016). PubMed PMID: 27764162. PubMed Central...
  2. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...expression of human IL1RN, the researchers saw an 11-fold increase in mRNA levels after 2 hr and a 400...control of endogenous gene activation. Nat Chem Biol 11:198–200 . https://doi.org/10.1038/nchembio.1753 Richter...
  3. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...12005-15. Hernandez et al., Autophagy 2013 Nov 1;9(11):1852-61. A quicker method for protein inactivation...variants. Klapoetke et al., Nat Methods 2014 Mar 11;(3):338-46. For more information and descriptions....3179. Martell et al., Nat Biotechnol 2012 Nov;30(11):1143-8.  A new tool for CRISPR gRNA validation...
  4. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...non-human primate brain. Nature Nanotechnology, 1–11. https://doi.org/10.1038/s41565-023-01419-x Deverman...cellular receptor for AAV-PHP.B capsids. PloS One, 14(11), e0225206. https://doi.org/10.1371/journal.pone.0225206...prioritization for cell type-specific enhancer design. eLife,11, e69571. https://doi.org/10.7554/eLife.69571 Liguore...Primates Compared with Mice. Molecular Therapy, 27(11), 2018–2037. https://doi.org/10.1016/j.ymthe.2019.07.017...
  5. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...Engler C, Kandzia R, Marillonnet S. PLoS One 2008;3(11):e3647. doi: 10.1371/journal.pone.0003647. Epub 2008...
  6. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...via a self-excising degron. Nat Chem Biol. 2015 Sep;11(9):713-20. doi: 10.1038/nchembio Nabet B, Roberts...
Showing: 81 - 100 of 146 results