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  1. CRISPR Plasmids - Xenopus

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially...surrounding the DSB is introduced along with the Cas9 and gRNA plasmids, the cell may instead repair the break ...single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can...

  2. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can...

  3. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...guide RNA (sgRNA). While the dCas9 protein provides the transactivation activity, the sgRNA component ...activators and sgRNAs can be separately transfected (pmax dCas9 activators [48223~48227] + sgRNA expression...CRISPR Cas9 gRNA plasmids from Dr. Rudolf Jaenisch's lab. CRISPR...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-on is an RNA-guided...expression vector or the linear DNA containing the sgRNA expressing fragment produced by PCR), or expressed from... via the links below: Table 1. dCas9 Activators sgRNA Dual Expression ID Plasmid 48236 pAC2-dual-dCas9VP48...expression construct expressing both dCas9VP48 and sgRNA from separate promoters. 48238 pAC152-dual-dCas9VP64...

  4. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Using catalytically...fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers...it is easy to implement due to the simplicity of gRNA design, programmable for different genomic loci,...efficiently visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity...

  5. CRISPR Plasmids - Purify Genomic Loci

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...genomic DNA bound by the gRNA. An epitope tag(s) can be fused to dCas9 or gRNA for efficient purification...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Identifying molecules...of any genomic sequence specified by a particular gRNA. In the enChIP (engineered DNA-binding molecule-...vivo. For CRISPR-based purification, design your gRNA sequence to direct dCas9 to a specific locus, avoiding...

  6. CRISPR Plasmids - Repress Gene Expression

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...using does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead...expression by interfering with transcription. Design your gRNA to target your gene of interest’s promoter/enhancer...

  7. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...choose does not also express a gRNA, you will need to use a separate gRNA expression plasmid to target ...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Catalytically dead...increase transcription of a specific gene. Design your gRNA sequence to direct the dCas9-activator to promoter...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...with paired gRNAs to lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution... 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target genomic locus...is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed ...it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are...marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used to image...labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol . . PMID: 27088723...

  9. CRISPR Plasmids - Parasites

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic parasites...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the... - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158...

  10. Cre-lox system

    Type
    Collection
    ... CMV Lentiviral Lasek 87682 AAV-U6gRNA1-U6gRNA2-TnT-Cre Cre and gRNAs cTnT AAV Pu 87694 pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2...60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Cre, luciferase, and sgRNA expression EFS... Zhang 60229 AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR Cre and sgRNA coexpression Cbh AAV Zhang...ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR Cre, KASH-tagged EGFP, and sgRNA expression...expression hSyn AAV Zhang 60820 pSECC Cre, Cas9 and sgRNA expression EF-1 alpha Lentiviral Jacks 60877 pMAZe... vector Cbh Lentiviral Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre Cre Chicken cardiac troponin T AAV Pu 134308...

  11. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...consider that wildtype Cas9 with optimized chimeric gRNA has high efficiency but has been shown to have off-target... is that you can express two different chimeric gRNAs with the Cas9 nickase which will together introduce...with efficiency similar to using a single chimeric gRNA. At the same time, the off-target effects are reduced...sequence, and the concentration of Cas9 and guide RNA (gRNA). These considerations only matter if the PAM sequence... plate, co-deliver into the cells with the Cas9/sgRNAs vector (like PX330). For large changes (>100bp ... a typical size (~ 5kb) donor plasmid with Cas9/sgRNAs vector, for one well in 24-well plate. A good example...of a typical size (~5kb) donor plasmid with Cas9/sgRNAs vector, for a one well in a 24-well plate. A good...

  12. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close...

  13. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully functional ...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and 2. a specific edit close to the gRNA PAM site. When a repair template is present, the...

  14. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3 is a unique ...off-target alterations and require only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR-...Cas11. Cascade is directed to target DNA using a gRNA and recruits Cas3. After nicking the non-targeted...

  15. CRISPR Plasmids - dCas9-FokI

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A catalytically inactive... break (DSB) at a specific sequence. Two unique gRNAs, binding ~15-25 bp apart, are required for dCas9...

  16. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To make targeted ... dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter or enhancer for your...

  17. CRISPR Plasmids and Resources

    Type
    Collection
    ...methods can be used to visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment...experiment based on a variety of factors. gRNAs gRNA plasmids to target specific genes.... : Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links...

  18. FlyCRISPR

    Type
    Collection
    ...Browse CRISPR Cas9 gRNA plasmids for drosophila research from the O'Connor-Giles, Wildonger, and Harrison...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Harrison Lab - ...replacement by homologous recombination. For the sgRNA, vector pMB60 allows in vitro production of RNA ...template: example : Resources: Protocol for generating gRNAs 106.5 KB flyCRISPR website contains additional information...information CRISPR Optimal Target Finder Identifies gRNA target sequences from an input sequence and checks...

  19. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base...Base editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within...

  20. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems...

  21. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts RNA-Guided Nucleases...consist of two components: a short ~100 nt guide RNA ( gRNA ) that uses 20 variable nts at its 5’ end to base...the target DNA. The Joung lab recently described gRNA and Cas9 expression vectors and validated the use...design of short oligonucleotides needed to construct gRNA expression vectors. More information about RGNs ...

  22. CRISPR Plasmids - RNA Targeting

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems...

  23. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ... dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR-associated...

  24. Bacterial Expression Systems

    Type
    Collection
    ...see here . pgRNA-bacteria 44251 CRISPR Stanley Qi Expression of customizable guide RNA (gRNA) for bacterial...can be combined with expression of a gRNA from a plasmid like pgRNA-bacteria to knock down endogenous gene...Marraffini Bacterial expression of Cas9 nuclease gRNA. For a more comprehensive list of our bacterial ...

  25. Plant Plasmids and Resources

    Type
    Collection
    ...assembly of one or more gRNA expression cassettes - Qi-Jun Chen. Cas9 and gRNA backbone for use in rice... different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR...Plants includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots...

  26. CRISPR Guide

    Type
    Guide
    ... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...conversions using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target site... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...amplify a specific sequence of DNA pegRNA P rime E dit gRNA; a specialized gRNA containing an additional primer...Cas enzyme and a gRNA specific to the gene of interest. The genomic target of the gRNA can be any ~20 nucleotide...interactions between the gRNA scaffold and surface-exposed positively-charged grooves on Cas9. gRNA binding induces...

  27. Plan Your Experiment

    Type
    Guide
    ...how to design your gRNA . Browse Plasmids: gRNAs Synthesize and clone desired gRNAs Once you have selected...enzyme and gRNA Species of promoter and expression pattern of promoter for Cas enzyme and gRNA Presence ...delivery of Cas9 and gRNA In vitro transcription reactions generate mature Cas9 mRNA and gRNA, which are then... Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and in vitro transcribed gRNA are combined...modify prior to designing your gRNA, as sequence variation between your gRNA targeting sequence and target...the associated gRNAs based on their predicted on-target and off-target activity ( see gRNA design software...containing validated gRNAs are now available through Addgene. These plasmids contain gRNAs that have been used...

  28. Lentivirus Plasmids

    Type
    Collection
    ...plasmids to express your CRISPR gRNA? Please see our CRISPR Empty gRNA plasmids table and search "lenti...

  29. Guide to Using Pooled Libraries

    Type
    Guide
    ...each plasmid contains a unique cDNA. In shRNA or gRNA libraries, each plasmid contains a unique gene targeting...library from the Moffat lab has over 175,000 different gRNA-containing plasmids). Amplifying and Using the Library... screen. The figure above is an outline of how a gRNA library is used. Below are the general steps to ...experimental and control cells Generate a list of gRNAs that disappear with addition of the selection mechanism...

  30. Plasmid Collections

    Type
    Collection
    ...libraries. Pooled Library Types cDNA shRNA CRISPR gRNAs Depositor Collections A modular set of plasmids ...

  32. Microbiology Resources

    Type
    Collection
    ...CRISPR resources, such as depositor protocols and gRNA design software. Addgene yeast CRISPR resources ...

  33. Worm Expression Resources

    Type
    Collection
    ...resources include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging...

  35. Zebrafish Plasmid Collection

    Type
    Collection
    ... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR...

  37. Rett Syndrome

    Type
    Collection
    ... selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of additional CRISPR resources...

  38. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase Type Promoter Description PI 60226 AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR Renilla...

  39. Addgene's eBook Collection

    Type
    Guide
    ...planning your CRISPR experiment and designing your gRNA and how to use pooled libraries in genome-wide screens...

  40. Retrovirus Guide

    Type
    Guide
    ...γ-Retroviral transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is...

  41. Promoters

    Type
    Guide
    ...also transcribes small RNAs, such as shRNAs and gRNAs. Resources Addgene's blog , including our popular...
Showing: 201 - 242 of 242 results