...For example, G-deleted rabies lacks the viral glycoprotein (G) gene, which is not required for the transcription...

...nucleoids 164499 EZH2-mEGFP AICSDP-83 mEGFP EZH2 Polycomb repressive complex 2 164500 POLR2A-mEGFP AICSDP...

...72268 xpress gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express...

...consisting of ECFP and EYFP connected by 1 flexible glycine- and serine-containing peptide linker (GGSGGS) ...

...17532 pLTR-G 2nd Envelope Expression of VSV-G glycoprotein (Indiana strain). Use with packaging construct...

...proteases.( 3 ) BSG - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily, binds to ...

...TCTAATTGGTCTGTACCTaaagtggccgctaaggAAGCCCCCTGGGCTGATA lycat TAL3482 & TAL3483 TCCTTCATCTTCATCCAGcggcgctgggaggacgACCGCACACACATGAGCA...

...smegmatis Rock NA Varies 176,286 Neelamegham Human GlycoGene CRISPR Library 140961 Knockout Human Neelamegham...

... in the CIDAR MoClo Parts Kit are available as glycerol stocks in a single plate with every plasmid or...

...how to generate a monoclonal cell line from a polyclonal pool of stable cells....how to generate a monoclonal cell line from a polyclonal pool of stable cells. Transducing cells with ...with lentivirus results in a heterogenous polyclonal population that varies in the number of integration ... as the lower expressing clones take over the polyclonal cell pool. Generating a monoclonal cell line ... Hemocytometer or other cell counter Reagents Polyclonal stable cell pool DMEM high glucose, Corning 10...FBS and 5 mL of 100X glutaGRO. Store at 4 °C. Polyclonal stable cell pool: see our protocol for generating...cell lines with lentivirus . Pro-Tip Because the polyclonal cell pool is always undergoing selection, it’...

...) 7-HCou Aminoacyl (7-hydroxy-4-coumarin-yl) ethylglycine Bacterial TAG Kenneth Johnson 198323 pRSF-G1TMSNKRS...

...cells, resulting in a more homogenous (but still polyclonal) cell population. Depending on the transducibility...regularly to confirm transgene expression. As polyclonal populations of resistant cells start proliferating...etc. Pro-Tip This selection method results in a polyclonal cell population, meaning that the transgene has...from different cells in the population. Once the polyclonal populations are growing well and have been sufficiently...expression. Over time, transgene expression in a polyclonal population may drop. This is because cells that...consider generating monoclonal lines from the early polyclonal populations. Sample Data Figure 1: Generation...

... simplifies the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series...

...from Bacterial Culture Introduction If you have a glycerol stock or stab culture of bacteria and need to ...burner. Obtain the approrpriate bacterial stab or glycerol stock . Using a sterile loop, pipette tip or toothpick...punctured area of the stab culture or the top of the glycerol stock. Pro-Tip If you use a wire loop you can ...

...Lithium Chloride (for precipitation step, Option B) Glycogen (Optional) Caution Make sure to read the SDS (...anticipate your RNA yield to be small, RNase-free Glycogen may be used as a carrier to facilitate RNA precipitation...

... GCaMP6s calcium sensor A polarized Ca(2+), diacylglycerol and STIM1 signalling system regulates directed...

...Bacteria on an LB Agar Plate Creating Bacterial Glycerol Stocks for Long-term Storage of Plasmids Recovering... the bacteria, you can proceed with Creating a Glycerol Stock . You can now isolate your plasmid DNA from...

...liquid medium Watch the Video! Creating Bacterial Glycerol Stocks Store bacterial strains or plasmids for...Dilution Generate monoclonal cell lines from a polyclonal pool of stable cells AAV Production in HEK293...

...Benzonase/DNAse I (Millipore 71205-3) 40% Polyethylene Glycol 8000 (PEG) + 0.5 M NaCl Cell lysis buffer (50 mM... and thaw a new working stock. 40% POLYETHYLENE GLYCOL (PEG) 8000 solution: Dissolve 400 g of PEG 8000...

...happen for a variety of reasons, including high glycerol concentration. Learn more at (Link opens in a ...

... migrated; 2) it contains a high percentage of glycerol that increases the density of your DNA sample ...

...freeze-thaw if not suspended in cryoprotectant like Glycerol or DMSO solutions. Quantify the individual Maxipreps...

...complex in the transduced cells. hPGK Human phosphoglycerate kinase promoter drives expression of puromycin...

...lower background signals than polyclonal antibodies. However, polyclonal antibodies are more sensitive...clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that are specific... antibodies are typically more expensive than polyclonal antibodies. Environmental factors, binding partners...available epitope on your protein of interest. Polyclonal antibodies are typically collected directly from...targeting various epitopes on the same antigen. Polyclonal antibodies are often more environmentally stable...protein in any form it may be present in. However, polyclonals can vary significantly from lot to lot, as immune...consider the following questions: Do you need polyclonal or monoclonal antibodies? Will you be using a...

...within bacterial cells that have been preserved as glycerol stocks. Functional in many species and can be ...Glutamine Gln Q CAA, CAG Glutamic Acid Glu E GAA, GAG Glycine Gly G GGU, GGC, GGA, GGG Histidine His H CAU, CAC...

...Transformation Plasmid Cloning Creating Bacterial Glycerol Stocks Restriction Digests Guides Browse our in-depth...

...Envelope VSVG in trans Vesicular somatitis virus G glycoprotein; Broad tropism envelope protein; Pseudotyped...

...promoter PGK Constitutive Mammalian promoter from phospholycerate kinase gene TRE Inducible Tetracycline response...

...Envelope VSVG in trans Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein used to psuedotype...

...coupled to: PSAM-Gly LGICs pair a PSAM domain with a Glycine-receptor (GlyR) chloride-selective IPD. Binding...