...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite...

...a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line and then transduce this...

...if not methylated (if it comes from a phage for instance). Aside from restriction modification systems,...

...medRxiv.org) in the past few years.   Fig. 1: Stand aside, CERN - it's a new age of accelerating science...

...workshop was well received, and then when the team started brainstorming ideas for the next workshop, they...

...method to create CRISPR/Cas9 constructs, assemble standard plasmids parts in different combinations, or other...

...organism where deletion strains are available. Then, staff or students clone similar sequences from a less-...

...2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving. Variants of Cas proteins can be used...

...away from a desired site does not have the same devastating impact on efficiency as 10-15 bps away does. ...

...In order to understand the complex wiring of the brain and the underpinnings of neurological disease,...

...aa 155), includes I152L in N-terminal fragment Constant rate of p53 tetramerization in response to DNA...

...where cleavage leads to the loss of spectinomycin resistance. xCas9 3.7 showed the broadest PAM range, recognizing...

...useful tools, and as such, we’re always looking to understand our community better. We therefore looked at ...

...translate perceived signals to physical actions. Understanding neuronal pathways and the function of certain...

...techniques do tip the scales in your favor. For starters, Golden Gate cloning is extremely efficient due...

...not a leader,” because you don’t see yourself  standing in front of a crowd and using your charisma to...

...and adaptations that can provide an increased understanding of DNA binding and gene regulation. For additional...

...medical journal. Decades later and after years of painstaking work trying to isolate the causative gene for...

...extremely important gene in animal development. To understand where sonic hedgehog got its name, we need to...

...IgG isotype standard 2.5 mg/mL - can use commercial standard or validate an in-house standard Before Starting...Thaw IgG standard and prestained protein ladder on ice. Reagent Preparation Prepare IgG Standard Dilute ...ready-to-use bio-safe Coomassie G-250 stain (Addgene uses SimplyBlue SafeStain) and ImageJ software. The sample... serial dilutions of a standard antibody of known concentration. After staining with Coomassie blue, protein...ready-to-use biosafe Coomassie stain and ImageJ software. Protocols... Protocols Coomassie Purity Stain of Recombinant Antibodies...Antibodies Coomassie Purity Stain of Recombinant Antibodies You may also like... Antibody Plasmid Collection...