We narrowed to 1,055 results for: sta

1. Hot Plasmids and Viral Preps - July 2021

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   Blog Post

   ...vitro and have emerged as a powerful model for understanding human physiology, development, and disease. ...

2. Antibodies 101: Affinity Tags

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   ... suited for their primary application. Ready to start tagging? We are here to help with vectors, protocols...

3. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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   ...built-in primer design tools, but here are a few stand-alone sites for general primer design, as well as...

4. New Viral Vectors - Fall 2024

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   ...Notes AAV-GfaABC1D-Cre-4x6T AAV5 Recombinases Stanley Thomas Carmichael New viral prep pAAV-EF1a-fDIO-Cre...

5. Build Your CRISPR Vocabulary

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   ...Resources on the Addgene blog A Needle in a Base-Stack: Cas9 Structural Biology...

6. Year of the Camelid: Antibody Style

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   ... early years of sdAbs development, the process started with immunizing a camelid (usually llamas or alpacas...

7. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

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   Blog Post

   ...data by biomedical researchers and ready access to stably archived large-scale datasets by computational ...

8. New CRISPR Web Resources and #12DaysofCRISPR Recap

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   ...Since the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that...

9. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   ...tackling this type of genetic engineering is a testament to how amazing the downstream payoff could be....

10. Choosing a Good Mentor for Scientists

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    Blog Post

    ...proficiency, communicate your work, maintain ethical standards and interact professionally with other scientists...

11. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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    Blog Post

    ...The lab saw that the B.1.351 variant was very resistant to vaccine serum responses. This result was similar...

12. Celebrate Open Data Day with Addgene’s AAV Data Hub!

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    Blog Post

    ... much-needed community resource. Instead of painstakingly searching for experimental details in research...

13. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

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    Blog Post

    ...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

14. New and Upcoming Viral Vectors - September 2019

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    Blog Post

    ...neurotransmitter - is a powerful method for understanding how this molecule impacts neuronal circuits....

15. Using CRISPR/Cas9 to Edit Disease Out of the Genome

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    ...genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first author of the cystic...

16. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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    ...references and web links from my first post and start designing your first fly experiment! Many thanks...

17. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post

    ...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....

18. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

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    Blog Post

    ... bacterial strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI)...

19. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

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    Blog Post

    ...that this enzyme does not cleave at a predictable distance from the targeted region. Konermann et al. show...

20. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post

    ...OptoNB can’t bind, it localizes to the cytosol. To start, the team inserted AsLOV2 into conserved sites in...