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Showing: 961 - 980 of 1044 results

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Year of the Camelid: Antibody Style

Type

Blog Post

... early years of sdAbs development, the process started with immunizing a camelid (usually llamas or alpacas...
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

Type

Blog Post

...tackling this type of genetic engineering is a testament to how amazing the downstream payoff could be....
Choosing a Good Mentor for Scientists

Type

Blog Post

...proficiency, communicate your work, maintain ethical standards and interact professionally with other scientists...
Celebrate Open Data Day with Addgene’s AAV Data Hub!

Type

Blog Post

... much-needed community resource. Instead of painstakingly searching for experimental details in research...
Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

Type

Blog Post

...The lab saw that the B.1.351 variant was very resistant to vaccine serum responses. This result was similar...
Even more elegant: Single injection CRISPR/Cas9 in C. elegans

Type

Blog Post

...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

Type

Blog Post

...references and web links from my first post and start designing your first fly experiment! Many thanks...
The Power Behind NGS Plasmid Validation: seqWell

Type

Blog Post

...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....
Using CRISPR/Cas9 to Edit Disease Out of the Genome

Type

Blog Post

...genomes of adult stem cells in organoid cultures are stable,” said Gerald Schwank, first author of the cystic...
New and Upcoming Viral Vectors - September 2019

Type

Blog Post

...neurotransmitter - is a powerful method for understanding how this molecule impacts neuronal circuits....
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

Type

Blog Post

... bacterial strain such as JM110 or INV110. Avoid star activity. Some endonucleases (for example BamHI)...
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Type

Blog Post

...OptoNB can’t bind, it localizes to the cytosol. To start, the team inserted AsLOV2 into conserved sites in...
Luminescent Imaging with Nano-lanterns

Type

Blog Post

...coelenterazine-h to coelenterazine-h, producing a constant supply of substrate. Using this compound to excite...
Top Requested Lentivirus and AAV of 2016

Type

Blog Post

...a LentiCas9-Blast viral prep to create a single stable Cas9-expressing cell line and then transduce this...
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

Type

Blog Post

...that this enzyme does not cleave at a predictable distance from the targeted region. Konermann et al. show...
GPCRs: How Do They Work and How Do We Study Them?

Type

Blog Post

...//doi.org/10.1038/nsmb.3014 Paek J, Kalocsay M, Staus DP, Wingler L, Pascolutti R, Paulo JA, Gygi SP, ...
Plasmids 101: Methylation and Restriction Enzymes

Type

Blog Post

...if not methylated (if it comes from a phage for instance). Aside from restriction modification systems,...
Viral Vectors 101: Viral Vector Elements

Type

Blog Post

...what goes into ‘making virus’ or what gag and pol stand for, we demystify all components of viral vectors...
Viral Vectors 101: Biosensors

Type

Blog Post

...Biosensors tend to be more reproducible when used as stable systems, rather than transiently overexpressing...
Reproducibility for Everyone: Lessons from an Open Science Collaboration

Type

Blog Post

...workshop was well received, and then when the team started brainstorming ideas for the next workshop, they...

Showing: 961 - 980 of 1044 results

Results per page:

Year of the Camelid: Antibody Style

Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

Choosing a Good Mentor for Scientists

Celebrate Open Data Day with Addgene’s AAV Data Hub!

Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

Even more elegant: Single injection CRISPR/Cas9 in C. elegans

Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

The Power Behind NGS Plasmid Validation: seqWell

Using CRISPR/Cas9 to Edit Disease Out of the Genome

New and Upcoming Viral Vectors - September 2019

Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

Luminescent Imaging with Nano-lanterns

Top Requested Lentivirus and AAV of 2016

Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

GPCRs: How Do They Work and How Do We Study Them?

Plasmids 101: Methylation and Restriction Enzymes

Viral Vectors 101: Viral Vector Elements

Viral Vectors 101: Biosensors

Reproducibility for Everyone: Lessons from an Open Science Collaboration

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