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...fragments described above can be used for similar applications and have similar advantages, though with some...

...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm...

...that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 fused to a transcriptional repressor...

... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...

...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide...

...Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4)...

... from mutations in the gene, and whether any publications have mentioned the gene. The Bloomington Drosophila...

...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...

...in line 4 of Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed...

...desktop. The Plasmids 101 eBook is designed to educate all levels of scientists and plasmid lovers and...

...Agrobacterium tDNA insertion likely precipitated domestication of sweet potatoes (Kyndt et al., 2015)! It sounds...

...other activities. Take care of yourself. Take a vacation alone with your partner – no kids. Get a massage...

...Mbp region of the mouse chromosome. Of the genes located in this stretch of DNA, Ly6a seemed the most likely...

...anticipated outcome, and payoff for each aim. Learn to communicate your story with only the most salient details...

... both accept deposit of data associated with publications into one of five general purpose open source...

...staining. Direct ELISAs also lack the signal amplification afforded by secondary antibodies and thus are...

... written up in mSphere. A post-meeting survey indicated that the program was largely a success, but also...

...many cell types and often results in biallelic modifications. Most commonly, DSBs are repaired by the nonhomologous...

...created by making some or all of the following modifications: 1) increased concentration of Taq polymerase...