... water, not tap water, to prevent salts from accumulating on the surfaces of the water bath as the water... your experiment. Water baths tend to heat up relatively slowly. Place the water bath cover on the top...

... (ITRs). Genes are divided into early (E1-4) and late (L1-5) transcripts. There are 57 accepted human ...construct, and virus can be harvested about 7-10 days later. Vogelstein designed multiple shuttle vectors for...non-enveloped double-stranded DNA viruses. They are not related; however, AAV requires the presence of adenoviral...

...hepatitis virus post‐transcriptional regulatory element; sequence that stimulates the expression of transgenes...-retroviral plasmids have similar names? The nomenclature of the plasmid backbone is meant to be descriptive...

.... Use a (Link opens in a new window) ligation calculator to easily quantify how much vector and insert...vector end. (Link opens in a new window) Ligation calculators are easily found on the web. Just enter the concentration...

... molecular cloning scientists can amplify and manipulate genes of interest and then insert them into plasmids...hundreds of available enzymes, many of which are relatively cheap. They also cut specific target sequences...and choice of restriction site. Because of the relatively long stretches of base pairing in the annealed...

...be in the liquid. Pro-Tip If pipetting from a relatively large container holding a small volume of liquid...liquid into different tubes or into wells of a microplate, you may consider using a multichannel pipette...

...Workflow Timeline Day 1: Purify antibody Day 2 or later: Buffer exchange Equipment Class II, Type A2 Biological...spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Clamp stand and...

...any of our eBooks to learn about popular topics related to our collections. Titles include: Plasmids 101...Read about plasmids in Addgene’s repository , and related molecular biology techniques. Topics include: Genome...

...start learning about various scientific concepts related to our plasmid collection. Find links to the guides...optics and genetic engineering to measure and manipulate cells (frequently neurons) and their governing...

...balance. This will help you remove it from the scale later on as otherwise, the paper could stick to the surface...

...sequence is used, plus a minimum of 18 bp of your template sequence. 5' and 3' primers will have different...

...lab. Ensure that a designated chemical waste accumulation site is present, and that you have received ...

...gel. Pro-Tip If you will be purifying the DNA for later use, use long-wavelength UV and expose for as little...

...you need to know about plasmids, from parts, nomenclature, and uses to common promoters and cloning techniques...

...the complementary DNA strand. Rep and Cap are translated to produce multiple distinct proteins (Rep78,...