We narrowed to 1,024 results for: cat

1. Antibodies 101: Buffers, Storage, and Conjugates

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   Blog Post

   ...compensation.    Working with antibodies can get a bit complicated, but hopefully this helps you successfully select...

2. CRISPR 101: Non-Homologous End Joining

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   ...Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous...

3. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

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   Blog Post

   ...This is a chance to practice leadership and communication skills. The leader also provides materials such...

4. dTAG - You're it!

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   ... and we will provide an update with vendor and catalog numbers. As we are happy to share aliquots of dTAG...

5. Transferable Skills Guide: Public Speaking

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   ...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm...

6. Plasmids 101: The protein expression toolbox

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   ...that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 fused to a transcriptional repressor...

7. Antibodies 101: Fab Fragments

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   ...fragments described above can be used for similar applications and have similar advantages, though with some...

8. Troubleshooting Your Plasmid Cloning Experiment

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   ...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

9. Antibodies 101: Stripping and Reprobing Western Blots

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   ... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...

10. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....

11. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ... from mutations in the gene, and whether any publications have mentioned the gene. The Bloomington Drosophila...

12. Plasmids 101: Knockout/Knock-In Plasmids

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    ...in line 4 of Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed...

13. Antibodies 101: Flow Compensation

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    ...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value...

14. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    ...HDR. Optimal mutation in-depth One the most sophisticated features of CrispyCrunch is how it mutates guide...

15. Fluorescent Tagging of Endogenous Genes with SapTrap

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    ...Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4)...

16. Plasmids 101: Multicistronic Vectors

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    ...desktop. The Plasmids 101 eBook is designed to educate all levels of scientists and plasmid lovers and...

17. Tips for arabidopsis transformation

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    Blog Post

    ...Agrobacterium tDNA insertion likely precipitated domestication of sweet potatoes (Kyndt et al., 2015)! It sounds...

18. Negotiating Work and Life: How to Find the Joy

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    ...other activities. Take care of yourself. Take a vacation alone with your partner – no kids. Get a massage...

19. Antibodies 101: The Four ELISAs and When to Use Them

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    ...staining. Direct ELISAs also lack the signal amplification afforded by secondary antibodies and thus are...

20. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

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    ... written up in mSphere. A post-meeting survey indicated that the program was largely a success, but also...