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Plasmids 101: Screening Strategies Used in Plasmid Cloning

Type

Blog Post

... of the entire plasmid. Please note that NGS verification is more time-intensive than Sanger sequencing...
Plasmids 101: Inducible Promoters

Type

Blog Post

...prokaryotic promoter often used for bacterial protein purification. When arabinose is absent, regulatory protein...
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

Type

Blog Post

...Immunosurveillance and Defense With increasingly sophisticated visualization techniques it is possible to follow...
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

Type

Blog Post

...interactions of proteins within these structures. Enzyme-catalyzed proximity labeling (PL) uses a promiscuous labeling...
CRISPR 101: Non-Homologous End Joining

Type

Blog Post

...Giovannangeli C, Jasin M, Brunet E (2014) Chromosomal Translocations in Human Cells Are Generated by Canonical Nonhomologous...
Plasmids 101: Screens vs. Selections

Type

Blog Post

...In fact, selections are often inappropriately categorized as screens! Why the confusion? The commonality...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

... be used interchangeably. Are γ-retroviruses replication incompetent? Yes, as with lentiviruses, the genes...
New and Upcoming Viral Vectors - May 2020

Type

Blog Post

...availability date or to be notified when a particular catalog item is available for order please email [email protected]...
Antibodies 101: Buffers, Storage, and Conjugates

Type

Blog Post

...compensation. Working with antibodies can get a bit complicated, but hopefully this helps you successfully select...
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

Type

Blog Post

...This is a chance to practice leadership and communication skills. The leader also provides materials such...
Transferable Skills Guide: Public Speaking

Type

Blog Post

...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm...
Plasmids 101: The protein expression toolbox

Type

Blog Post

...that! CRISPR interference (CRISPRi) targets a catalytically dead Cas9 fused to a transcriptional repressor...
Troubleshooting Your Plasmid Cloning Experiment

Type

Blog Post

...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...
Antibodies 101: Fab Fragments

Type

Blog Post

...fragments described above can be used for similar applications and have similar advantages, though with some...
dTAG - You're it!

Type

Blog Post

... and we will provide an update with vendor and catalog numbers. As we are happy to share aliquots of dTAG...
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Type

Blog Post

... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging....
Antibodies 101: Stripping and Reprobing Western Blots

Type

Blog Post

... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...in line 4 of Figure 4. Since the loxP sites are located in intronic regions, this gene will still be expressed...
Fluorescent Tagging of Endogenous Genes with SapTrap

Type

Blog Post

...Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans.” Genetics. 202(4)...
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

Type

Blog Post

... from mutations in the gene, and whether any publications have mentioned the gene. The Bloomington Drosophila...

Showing: 901 - 920 of 989 results

Results per page:

Plasmids 101: Screening Strategies Used in Plasmid Cloning

Plasmids 101: Inducible Promoters

Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

CRISPR 101: Non-Homologous End Joining

Plasmids 101: Screens vs. Selections

Viral Vectors 101: Gamma-Retroviral Packaging Systems

New and Upcoming Viral Vectors - May 2020

Antibodies 101: Buffers, Storage, and Conjugates

Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

Transferable Skills Guide: Public Speaking

Plasmids 101: The protein expression toolbox

Troubleshooting Your Plasmid Cloning Experiment

Antibodies 101: Fab Fragments

dTAG - You're it!

CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

Antibodies 101: Stripping and Reprobing Western Blots

Plasmids 101: Knockout/Knock-In Plasmids

Fluorescent Tagging of Endogenous Genes with SapTrap

Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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