...chain and light chain for the antibody and when translated into protein will assemble into a fully functional...

...researchers to select large sets (with overhangs that violate some of the traditional design rules) for successful...

...fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen...

...-scientists in an unusual setting: Discuss your latest research in a local pub at a Pint of Science event...

...Other” column. For example, you may enter “donor template vector”. Cloning Method Choose from: Gateway...

...purified secreted rAAV for rapid and cost-effective manipulation of gene expression in the CNS. Molecular Neurodegeneration...

...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add...

...tendency to runout right when you need them and to accumulate a bunch of unused buffers because you’ve run ...

...helpful information about the subject, link to related guides and protocols, and provide easy to sort ...

...maintaining the structure of your samples, the relationships between cellular components, and the accessibility...

...(CRISPRi) or an activation domain (CRISPRa) to regulate gene expression. Some CRISPR libraries contain...

... deliver a large amount of DNA (~8 kb) with a relatively low immune response, it should come as no surprise...

... yourself a Scientist. You work in a lab. You formulate hypotheses and conduct experiments. You transfer...

...cells with plasmids encoding aptamer-tagged RNA and later introducing the small molecule into the cell. The...

...courses on technologies and laboratory techniques related to antibody discovery. You can find cell surface...

...that’s only good once. Even when things return to relative normalcy, I’ll be incorporating that mobile monitor...

...most commonly used in the laboratory for genome manipulation. When transposons are used in the lab, the transposase...

...’re working with and better understand how to manipulate it. Happy CRISPRing!   Molecular graphics and...

... an automated ELISA plate washer. Seal the plate. Place the plate on a microplate shaker set at 400 rpm...use an automated ELISA plate washer. Seal the plate Place the plate on a microplate shaker set at 400 rpm... the plate with a plate seal and wrap in foil. Incubate plate at room temperature on a microplate shaker...each row of a 96-well ELISA microplate. Cover the plate with a 96-well plate seal and incubate at 37 °C... to each well. Cover the plate with a plate seal and incubate on a microplate shaker set to 400 rpm for...

...overnight. Plate 2.5 mL of the transformed cells on each of the eight bioassay plates (two plates per tube...Antibiotic 245 mm bioassay plates (Molecular Devices, X602) Pro-Tip Pour these plates at least one day in advance... Agar + Antibiotic plates at 37 °C. Prewarm 8X LB Agar + Antibiotic Bioassay plates. Prechill Micropulser...100 dilution). Plate 100 µL of each dilution onto a prewarmed Petri dish. Incubate plates at 30 ℃ overnight... edge that can scrape plates more abrasively at a certain angle. Incubate plates upside down at 30 ℃ overnight... are not present on the most dilute plate, count the second plate (ie. the 1:10,000 dilution). After 12...LB to scrape bioassay plates and remove bacteria. Use one scraper for all plates. Use two 10 mL pipettes...