Transferable Skills Guide: Problem Solving
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...able to recognize it as a part of your skill set later on.
If you are new to research and the lab, you ...
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Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...Optogenetics and chemogenetics are powerful tools to modulate the activity of neurons and other brain cells.... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...specific audience. For example, I joined this effort later in the year and in the fall of 2018, I helped organize... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post... succeed. Thanks to all the Addgenies and congratulations to current and alumni Addgenies on our success... -
Summer SciComm Series: Cognitive Load
TypeBlog Post...require any detailed retention of information or formulation of new knowledge than it is to read a manuscript... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...is steadily increasing. Of course, you want the ‘latest and greatest’ variants with the best properties... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...for non-cytotoxic proteins which retain their regulatory functions.” Mol Microbiol. 1995 Mar;15(6):1031... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog Post...attention to the keywords used and think of ways to relate the proposal and deliverables to the aims requested... -
Progress Towards a PAM-Free CRISPR
TypeBlog Post...SpG Cas9 with the same PAM preference of NG was isolated by the Kleinstiver Lab (read more on this lab'... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog Post...researchers to select large sets (with overhangs that violate some of the traditional design rules) for successful... -
How to Prepare for an Industry Interview
TypeBlog Post...Congratulations, you’ve just landed an interview for an industry job! You’ve worked hard to earn this... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...chain and light chain for the antibody and when translated into protein will assemble into a fully functional... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...-scientists in an unusual setting: Discuss your latest research in a local pub at a Pint of Science event... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post...fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...Other” column. For example, you may enter “donor template vector”. Cloning Method Choose from: Gateway... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post...purified secreted rAAV for rapid and cost-effective manipulation of gene expression in the CNS. Molecular Neurodegeneration... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...want to include a homology directed repair (HDR) template to create knock-ins, point mutations, or to add... -
DNA Purification Without a Kit
TypeBlog Post...tendency to runout right when you need them and to accumulate a bunch of unused buffers because you’ve run ... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog Post...(CRISPRi) or an activation domain (CRISPRa) to regulate gene expression. Some CRISPR libraries contain... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog Post...helpful information about the subject, link to related guides and protocols, and provide easy to sort ...