Addgene

We narrowed to 950 results for: Cre

Showing: 881 - 900 of 950 results

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Plasmids 101: Methylation and Restriction Enzymes

Type

Blog Post

...keep in mind that dam-/dcm- strains may have an increased rate of mutation (as these would also be deficient...
REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

Type

Blog Post

...harnesses the power of bacterial recombineering to create insertions, deletions, and substitutions - at the...
CRISPR 101: Non-Homologous End Joining

Type

Blog Post

...Genome-Wide Control for Large-Scale Functional Screens ...
Will You Be My Mentor? Finding and Asking for Mentoring Support

Type

Blog Post

...can make a big difference, if you can find one. Create your own peer mentoring circle. This type of program...
Antibodies 101: Conjugation

Type

Blog Post

...protein-protein interactions with other peptides. Created with BioRender.com. Many biologists can...
CRISPR 101: Cas9 vs. The Other Cas(s)

Type

Blog Post

...limitations. New efforts have recently been made to increase cutting efficiency and diversify PAM sequences...
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Type

Blog Post

...proteins are available, and their number is steadily increasing. Of course, you want the ‘latest and greatest...
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Type

Blog Post

...more efficient, as one does not have to thoroughly screen colonies for the insert. Gateway® technology (developed...
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Type

Blog Post

...inhibition. To assess TRIM21 toxicity, Clift et al. created stable cell lines overexpressing TRIM21, finding...
Visualizing Protein Turnover In Situ

Type

Blog Post

...neurodegeneration. The level of this protein is decreased in Alzheimer’s disease patient’s brains. However...
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Type

Blog Post

...Roth BL (2007) Evolving the lock to fit the key to create a family of G protein-coupled receptors potently...
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Type

Blog Post

...toolbox, Ed Boyden’s lab developed a way to rapidly screen thousands of proteins for one that can report electrical...
Reproducibility for Everyone: Lessons from an Open Science Collaboration

Type

Blog Post

...highlights of 2018 for me was to collaborate with this incredibly dedicated team. Many thanks to Tyler Ford, Caroline...
Working with Nuclear Receptors

Type

Blog Post

... double-edged sword, however, and has greatly increased the burden associated with identifying plasmids...
Lab Automation at Addgene

Type

Blog Post

...Well, it used to be a lab assistant, but with increased plasmid demand it was too time consuming to complete...
A Look Back at One Year of Plasmid Sharing for COVID-19 Research

Type

Blog Post

...Hsu PD (2021) Genome-wide, bidirectional CRISPR screens identify mucins as critical host factors modulating...
How to Prepare for an Industry Interview

Type

Blog Post

...hard during this talk since you’ll be sharing your screen and won’t be able to see who’s asking a question...
Meet an Addgenie: Insights into Grad School, Career Paths, and More

Type

Blog Post

...change gave her the balance she needed. It is incredibly helpful to me to know that you can make big changes...
Split Fluorescent Proteins for Studying Protein-Protein Interactions

Type

Blog Post

...acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation...
Adapting Toehold Switches to Detect Zika Virus

Type

Blog Post

...parallel. More broadly, we drew on the combined creativity and expertise of the group and yet never hesitated...

Showing: 881 - 900 of 950 results

Results per page:

Plasmids 101: Methylation and Restriction Enzymes

REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

CRISPR 101: Non-Homologous End Joining

Will You Be My Mentor? Finding and Asking for Mentoring Support

Antibodies 101: Conjugation

CRISPR 101: Cas9 vs. The Other Cas(s)

Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

Visualizing Protein Turnover In Situ

Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

Reproducibility for Everyone: Lessons from an Open Science Collaboration

Working with Nuclear Receptors

Lab Automation at Addgene

A Look Back at One Year of Plasmid Sharing for COVID-19 Research

How to Prepare for an Industry Interview

Meet an Addgenie: Insights into Grad School, Career Paths, and More

Split Fluorescent Proteins for Studying Protein-Protein Interactions

Adapting Toehold Switches to Detect Zika Virus

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