Skip to main content
Addgene
Showing: 861 - 880 of 1028 results

  3. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...vivo studies should always take DNA’s chromatin status into account (Fig 1a). The laboratory of Karmella...dCas9 expression and contain different antibiotic resistance markers. New CRISPR pooled library from Keith...

  4. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...considered "Cre-On" meaning that your gene of interest starts in the inverse/antisense "off" position and is ...employed for "Cre-off" (i.e. your gene of interest starts in the sense orientation and Cre is used to flip...

  5. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...light up endogenous genes of interest to better understand normal protein function. However, CRISPR protein...introduced directly into a Drosophila S2 cell line stably expressing Cas9. This PCR toolkit offers C- and...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...CNS and PNS! What is the CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution...number of neurons overall (GFP+ cells that also stained for a neuronal marker). Check out tables 1 and ...

  8. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...is carefully designed primers. The majority of mistakes are due to incorrect primer sequences! Primers...transduction, and uses electron cryo-microscopy to understand receptor function.     Follow them on Twitter...

  11. MXS Chaining

    Type
    Blog Post
    ... (MCS). Modules are assembled one at a time by standard restriction enzyme digestion followed by ligation...Goler JA, Arkin AP, et al. BglBricks: A flexible standard for biological part assembly. Journal of biological...

  12. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ... should not even be the real goal. We are all constantly negotiating our energy and attention. The goal...going away to a conference. Really. No one will starve while you are away a few days. Also, your children...

  15. Hot Plasmids - August 2020

    Type
    Blog Post
    ...fluorescently labeled piggyBac vectors for uniform and sustained expression of single or multiplexed gRNAs and ...includes a CRISPRi-based synthetic oscillator, bistable network, stripe pattern-forming incoherent feed-forward...

  16. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...tool for sharing ideas. This leads to everyday obstacles for many of us, such as finding time to read all...platforms (such as social networking, visual appeal, instant sharing across multiple other sites), I created...

  17. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...prevent your gene from being up-regulated. For instance, generally, the more highly expressed a gene is...whichever guides are closest to the transcription start site (TSS). We recommend targeting the guides to...

  18. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...yourself up for a screening success? Before you start analyzing individual clones, it’s wise to quickly...can ensure you aren’t looking for a needle in a haystack before going on to generate and screen individual...

  20. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... to lab. Promega supports the Addgene mission statement: Accelerate research and discovery by improving...evolution of the coelenterzine substrate to the more stable yet brighter furimazine by Promega’s Chemistry ...
Showing: 861 - 880 of 1028 results