Skip to main content
Addgene
Showing: 841 - 860 of 1028 results

  1. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ...many cases the plasmids themselves are only the starting point for the production of viruses used to deliver...stocks. Our research scientists have provided their standard lentivirus production protocol here and you can...

  2. Components of CRISPR/Cas9

    Type
    Blog Post
    ...xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG  Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N)  ...the different components of CRISPR, It's time to start thinking about how you can used them in your experiments...

  3. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between...components, removing inducible promoters that could crosstalk with other pathways. They also reduced system ...

  5. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...GCE4All, an NIH-funded center located at Oregon State University focused on turning GCE tools developed...in your experiments, GCE4All is a great place to start. They host monthly webinars, workshops, and a bulletin...

  6. Antibodies 101: ChIP

    Type
    Blog Post
    ...may be thinking, well, these are mostly fairly standard molecular and cell biology techniques. What’s ...step can affect the optimization of another. For instance, a sample may need more or less cross-linking ...

  7. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ... and of our data in particular and our deep understanding of Addgene’s customer’s needs. Categories We...engine to be so specialized that it appears to “understand” biology.  We hope you will give our new search...

  8. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    ... are positive for your marker would be a good statistic to compare between samples. In cases where there... immunotherapies. She is currently a Research Assistant in Arlene Sharpe’s lab at Harvard Medical School...

  10. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...only 44% and 66% indel frequency with the use of standard single gRNA constructs. When PTG constructs with...<200 bp tRNA-gRNA takes up half the space of a standard pol III-gRNA, which will facilitate CRISPR/Cas9...

  11. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...intervening DNA (“pop-out” deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to...References Bennardo N, Gunn A, Cheng A, Hasty P, Stark JM (2009) Limiting the Persistence of a Chromosome...

  12. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...several considerations. First, it is important to establish the type of audience you want to reach with your...and help you correct any language- and spelling mistakes. A manuscript that is well written looks more ...

  13. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...DNA-protein interactions or to analyze chromatin states (CHIP-seq, FAIRE-seq, Cut & Run) and to detect ...interactions. To address this need, the Khavari lab at Stanford created the RNA-protein interaction detection ...

  14. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...resulting in GFP signal. With the addition of standards, the assay is semi-quantitative and can approximate...plasmids and suitability of various antibiotic resistance markers, promoters, and inducible gene expression...

  15. All in a Twist: dsRNA

    Type
    Blog Post
    ...However, transcription of thousands of genes is constantly occurring throughout a single cell, so these ... these structures can in turn lead to genome instability. Thus, it’s important to strike a balance between...

  17. Antibodies 101: Conjugation

    Type
    Blog Post
    ...possible antibody conjugates (especially if you start to consider clinical applications). But there are...make the process quick and easy. Before getting started, be sure to check that your antibody’s buffer is...

  18. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...use electroporation, a technique that briefly destabilizes the cell’s outer membrane and allows the components...structures and processes and can lead to the better understanding of cell biology. We hope that these high-quality...

  19. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ... effect different perturbations. Consider, for instance, the challenge of attempting to simultaneously...without making any functional changes to Cas9. For instance, when native (nuclease-competent) Cas9 interacts...

  20. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...that treat disease at the molecular level. For instance, if a disease is caused by a mutation in a single...developing AAV-based therapies. Furthermore, deeper understanding of disease pathogenesis will enable robust therapeutic...
Showing: 841 - 860 of 1028 results