Skip to main content
Addgene
Showing: 821 - 840 of 989 results

  2. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...The glycoprotein will be incorporated into the replicating rabies virus envelope, travel retrogradely, and... Pseudotypes: Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene...

  3. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ... (2014) A Protein-Tagging System for Signal Amplification in Gene Expression and Fluorescence Imaging.....org Find Cas9 Activators Find Cas9 Repressors Catch Up on Your CRISPR Background with Our CRISPR Guide...

  5. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ... conferences are held every year in different locations all over the world. But simply attending the conference...work. Addgene’s very own Joanne Kamens, a strong advocate for career planning, has written a great blog ...

  6. Lab to Office Culture Shock

    Type
    Blog Post
    ...involves a few key features that can be difficult to replicate in other work settings: The sense of being the... might include collaborating, managing, and communicating. After years of focusing on my technical skills...

  7. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...ribosome may therefore stall at inappropriate locations or fail to make it through the entire transcript...generally segregate with the cell pellet during purification procedures. Even if you produce a large amount...

  8. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...have made great strides to map brain regions and allocate specific functions to them. Electrical recordings...long-lasting antidepressant effects." Nature communications 10.1 (2019): 223. PubMed PMID: 30644390. PubMed...

  9. How to Be an Excellent Trainee

    Type
    Blog Post
    ... check out Addgene’s blog, eBooks, and other educational resources to help you get started!) Are there...something. It can be intimidating to ask for clarification at first, but in the end everyone wins. Your...

  10. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ...allows the detection of molecules' interactions, modifications or dissociations in situ. Used since the mid...14. Zimmermann T,  Terjung S. FRET Basics and Applications: An EAMNET Teaching Module http://www.embl.de...

  11. Five Popular Model Organisms

    Type
    Blog Post
    ... throughout their lifetime. Thus any genetic modification, such as expression of fluorescent protein or...studying somatic mutations and cancer." Nature Education 1.1 (2008): 183. Zuber, Johannes, et al. "Mouse...

  13. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...the user. This approach is useful for several applications as will be discussed below. The software could...contain multiple gRNAs targeting all genes in the indicated species and were suggested to be the best gRNAs...

  14. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...reporter, such as GFP or mCherry, through genetic modification. As your protein of interest will only occur...excitation and emission. The visible color spectrum is located in a range of wavelengths of about 380–700 nm. ...

  19. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...On the ninth day of CRISPR, Addgene gave to me: MUCICAT to perform multicopy chromosomal integration.  ...

  20. We’re Going Viral This Week!

    Type
    Blog Post
    ... information on the basics of viral vectors, applications, and some new resources available from Addgene...
Showing: 821 - 840 of 989 results