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TypeBlog Post... “species on species”) immunochemistry (IHC) applications an issue? The culprits are endogenous IgGs in...Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to Mouse Tissue...
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Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...launching a company without partners. You need a dedicated team that you know well and trust. This will ...Chowdhury and Clinton Buell, who have very diverse educational backgrounds (an essential component to any successful... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post... interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease...library of characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer Cruz-... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog Post...will you meet? How does the Mentor prefer to communicate between meetings (email, phone, etc.)? Make agendas...skills Support inclusion, diversity, and open communication Feel valued by giving back Final tips for success... -
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering
TypeBlog Post...brain, which has humanistic and philosophical implications. As an example, a group at Caltech used an optical...a handle on some of these mysteries. Another application is clinical; can we find ways to treat patients... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog Post...research papers or dissertations. Phrases are categorized either based on the section of a manuscript they...introduction, methods, results; or phrases are categorized by the goal of the phrase, such as being cautious... -
Celebrate National Book Month with Addgene
TypeBlog Post...sometimes we don't." - Jennifer Tsang, Science Communications and Marketing Coordinator Non-science favorites...times." - Aliyah Weinstein, Marketing and Communications Manager Addgene's book club Indeed, reading... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...plasmids at Addgene Opperman J et al., Nature Communications, 2019. PubMed PMID: 31346176. Using LADL to...light-activated-dynamic-looping (LADL) system. The group fused dCas9 to a truncated CIB1 protein from Arabidopsis (CIBN), which they... -
The MTA at Addgene
TypeBlog Post...things being organized, tracked, and clearly communicated. And that’s where MTAs, and similar agreements...portal automates a lot of the process, from notifications and emails to generating the UBMTA link for ... -
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**
TypeBlog Post...generation when bound by the dL5** FAP. The iodine-modifications also shift MG-2I’s excitation wavelength to ...FAP-TAP system works. Find the Plasmids from the Publication Here Light-induced cell ablation with MG-2I-dL5... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog Post...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...lines. In the meantime, be sure to browse the publication references and web links from my first post and... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog Post...smaller fragments that are then barcoded for identification. This mixture of barcoded fragments is known...of Toronto. He is an author of 20+ scientific publications and patents. ... -
With Our New Viral Service, We're Taking CRISPR Further!
TypeBlog Post...represent the fraction of that total with the indicated function. One huge reason CRISPR has become such...achieving the gRNA representation recommended in the publications associated with these libraries. Please note... -
How to Lead a Great Meeting
TypeBlog Post... dialogue and community among participants, clarification - new and useful information, and invigoration...be the representative in the meeting and then communicate outcomes to the rest of their team. Here's a ... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post... I hope these tips demonstrate that plasmid verification is not just necessary but also an easy process.... Please vist Addgene's resource for plasmid verification to find additional tips and detailed protocols... -
Antibodies 101: Isotypes
TypeBlog Post... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of these five isotypes, such as the number and location of disulfide bonds, the number of constant domains... -
Antibodies 101: Flow Cytometry Controls
TypeBlog Post...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color... -
Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies
TypeBlog Post...beforehand on which chromosome your docking site is located). This amounts to a small difference of two or ...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...Resources Find CRISPR Plasmids for Your Research Catch up on Your CRISPR Background with Our Guide Pages... -
Top Requested Lentivirus and AAV of 2016
TypeBlog Post... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics ...