We narrowed to 946 results for: omp
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TypeBlog Post...Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios of the viral...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...
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How to Write a Scientific Review Article
TypeBlog Post...Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases...Addgene.org Check out Our Hot Plasmids Articles to See Compiled Info on New Plasmids Review the CRISPR Field with... -
Mesothelioma - Causes, Symptoms, and Treatment
TypeBlog Post...is a terrible disease that is, unfortunately, completely preventable. Mainly caused by exposure to asbestos...non-resectable mesothelioma by the National Comprehensive Cancer Network, a coalition of the top 27 cancer... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...institutions doing? Institutions are now preparing to comply with the changes to the law on Dec 1st, according...centrally administered, which leads to many complications, including the inability of some institutions... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...ml before the media becomes cloudy. Mycoplasma compete with host cells for nutrients and can alter expression...al. outline a do it yourself PCR-based approach complete with freely available positive controls. Routinely... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...membrane-impermeant and can only enter cells with compromised plasma membranes — i.e., dead cells — while live...which yields a much weaker signal on live cells compared to dead cells. As a result, the largely negative... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...Tarlow, Branden D., Milton J. Finegold, and Markus Grompe. "Clonal tracing of Sox9+ liver progenitors in ... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...enhanced brightness, expression, and sensitivity compared with earlier SF-iGluSnFR. Image from Aggarwal ...size and reduced sensitivity to pH and calcium compared to previous lactate biosensors, and its affinity... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post...HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing...mutation. Here, the blocking-only templates will compete with the blocking/intended mutation templates resulting... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...undetectable in the blood. But the virus doesn’t completely disappear; it just hides in latently infected...viral reservoirs, and CRISPR may be the way to accomplish this tough job! Kamel Khalili’s lab at Temple... -
Viral Vectors 101: AAV Serotypes and Tissue Tropism
TypeBlog Post... cell types. Figure 1: Diagram of AAV components. Created with BioRender.com. AAV vectors ...Transduction of Airway Epithelial Cells in Mouse Lungs Compared to That of AAV2 Vectors. Journal of Virology, ... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...enhancer regions (Mendenhall et al., 2013). By comparing gene activation when enhancers were active or ...increased gene activation at the targeted site compared to other Tet1 delivery systems. Transcriptional... -
Hot Plasmids: Spring 2025
TypeBlog Post... domain structure. Glypicans are proteoglycans composed of a protein core, 2–4 glycosaminoglycans (GAG...). Figure 5: TurboCas protein labeling compared to immunoprecipitation. The top panel depicts ... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...sites are both recognized by Cre, but they are comprised of different sequences (loxp and lox2272) and ...essential that every new vector, whether it be a completely new transgene or merely a new lot, be thoroughly... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post...experiences! These applications can be highly competitive, so asking for help from mentors and other students... Imposter: The Effect of Perceived Classroom Competition on the Daily Psychological Experiences of First-Generation... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...direct injection of a CRISPR/Cas9 ribonucleoprotein complex with linear DNA as repair templates which reduces...worms are perfectly viable at 15 ºC, yet exhibit complete embryonic lethality at 25 ºC. This method resulted... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...parameter to consider is the contrast ratio- a comparison of PA-FP brightness before and after photoactivation...variants are more monomeric than mEos2 (mEos4b is completely monomeric) and display bright and photostable... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...vitro Both in vitro and in vivo Epigenetic marks Complete removal to convert to iPSCs and re-establishment...in science communication and publishing after completing her postdoctoral training from Massachusetts ... -
New Coomassie Protocol Video
TypeBlog Post... If this video is helpful, you can find our complete collection of protocol videos on the Addgene YouTube... -
New Western Blot Protocol Video!
TypeBlog Post...That's why we gave our written protocol a video companion! Follow along as Addgenie Meghan and Abi run a...