...RNA sequence, they adopt an enzymatically active state and can bind and cleave additional RNAs regardless...homology to the crRNA. This activity provides a stark contrast to Cas9 and Cpf1, which require that each...

... Protein Plasmids by Category Empty Backbones Understand the localization and/or function of your protein...fluorescent protein. FRET Find FRET pairs and standards to study protein-protein interactions or conformational...

...Posts Eukaryotic parasites continue to cause substantial death and disease in the world. Parasites such..., CRISPR-dCas9 gene regulation has also been established for some of these parasites. Together, these ...

...plasmids and science information you need before starting your experiment. Genome Engineering Genome Engineering...Communities Find a scientific community that has established a common place for deposited plasmids. ASAP Discovery...

...below or check out our CRISPR resources on how to start using CRISPR in your lab. Addgene CRISPR Resources...nickase enzymes fused to reverse transcriptase install new genetic information into a specific DNA site...

...to a reverse transcriptase (RT) is capable of installing targeted insertions, deletions, and all possible...gRNA Plasmid Promoter Cloning Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species...

...H840A nickase. The fusion enzyme is capable of installing targeted insertions, deletions, and point mutations...protection factor La to PEmax to enhance pegRNA stability epegRNA - additional protection added to 3’ tail...

... of pMKO.1 puro with GFP instead of puromycin resistance gene William Hahn Additional Resources Web References...Mar; 33(3):401-6. PubMed . Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart...

...and responsible innovation for improvement of sustainable agriculture and conservation. Medicinal Plant...in a new window) - The Taproot is a podcast to understand how scientific publications are created. Papers...

...interaction with negative regulators, increase its stability and DNA binding activity, and allow it to bind...regulator; also known as MDM4 Noxa Phorbol-12-myristate-13-acetate-induced protein 1; also known as PMAIP1...

...Martin-Luther-University Halle-Wittenberg and Adam Bogdanove at Iowa State University published the nucleotide recognition ...some restrictions (for example, the target must start with a T), but they still have potential targetable...

...retroviral expression vector with the puromycin resistance gene 63591 3xFN-Tel-TAL/pMXs-neo Expresses a ...retroviral expression vector with the neomycin resistance gene 64325 3xFLAG-dCas9/p-bacteria Expresses ...

...proteins are excellent tools for further understanding the function of your favorite gene. For example...experiments, scientists often want to create stable cell lines in which the plasmid is incorporated...

...TGGACGCTCTGGTAATGGcacgggattgacacctCTACAGGTGGGCAGCACA stac TAL3184 & TAL3185 TGCAGAGGCTAAAGCGTTcgctgtccttcaagacCAAGAGTCTTCGCAGCAA Stat3 TAL3560 & ...

...transgenes in Cre transgenic lines. As part of our standard viral production , these viral vectors undergo... plasmid with PCR, purity assessment by silver staining, in vitro and (when possible) in vivo testing,...

...choose to start their own academic research laboratory. How does one even go about starting a lab? Obviously...students and postdocs when starting out. She advises “do not hurry to staff up the lab and to take things...resources for hiring research staff. And for a more indepth resource for starting your academic lab, download...a PI without a personal assistant has to deal with. My inbox currently stands at about 65,000 messages...for advice on staffing your lab. Let us know if you have any relevant tips for starting a lab. Or share...effective working environment? Tom Ellis was offered a start-up package that included a postdoc and a PhD student...important to me to be with them in the lab when they started, so that we could discuss our projects and ideas...

... you. Statistics – The basics Statistical analysis is what determines if there is statistical significance... several core factors of statistics relevant for many downstream statistical tests.   Independent variables...are statistically different. They are classic parametric tests and one of the simplest statistical tests...described above. Violated assumptions and statistics Statistical power, the ability to correctly identify...is a better option. Stay tuned for our upcoming blog on non-parametric statistical tests! Adding it all...your friendly neighborhood biostatistician happy by making your statistical analysis plan when you design...Wissing, Dennis R., and Timm, Donna. Statistics for the nonstatistician: Part I. South Med J., 105(3): 126...

... creation of the United States Environmental Protection Agency, which started making regulations such ... the second in a series of posts on bringing sustainable practices in the lab. The first post focused ...learn about some creative approaches to lab sustainability, and find ways to further reduce our consumption...consumption beyond recycling.     Choosing more sustainable products Recycling is great for rerouting waste... grant agencies), we can shift toward a more sustainable one. The math on this is pretty simple: wash ...solutions and practices to meet higher environmental standards. Find some easy action items on our other article...

...location where the United States Postal Service picks up mail. NEB actually started this program in 1976! ..., flip the flaps of the box so the pre-paid postage stamp is on the outside of the container, and drop...off the box at any location where the United States Postal Service picks up mail. Recycle plastic packaging...more lab sustainability tips, check out our other articles in this series: Bringing Sustainable Practices...popularity of single-use items, plastic is a substantial portion of lab waste. Despite the fact that researchers...cost of regular waste services. Learn about how to start a glove recycling program at your institute! What...culture room for the wrappers and ship them back. Start a glove recycling program at your institute! Universities...

...involved, it can be difficult for scientists to understand how GPCR activation leads to downstream effects... the endogenous ligand or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2...resulting in a decreased GFP2 emission. The activation state can be measured by a BRET2 ratio, which is the ratio...pharmaceutical drugs, there is still only a partial understanding of how GPCRs signal through G protein signal...provides scientists with a toolkit to gain an understanding of the signaling through the transducerome from...as well as the activation by drugs to further understand their mechanism. These plasmids are available... the transducerome, to gain a more complete understanding of a GPCR mediated response.  Acknowledgements...