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  1. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also ...transcription/ general expression Promoter from T7 bacteriophage Constitutive, but requires T7 RNA polymerase... levels of gene expression Promoter from T7 bacteriophage plus lac operators Negligible basal expression...transcription/ general expression Promoter from Sp6 bacteriophage Constitutive, but requires SP6 RNA polymerase...High levels of gene expression  Promoter from bacteriophage lambda Can be temperature regulatable  Often...transcription/general expression Promoter from T3 bacteriophage Constitutive, but requires T3 RNA polymerase...

  2. Immunology Research Plasmids and Resources

    Type
    Collection
    ...the Michael J Fox Foundation is a synuclein phagemid VHH phage display library used to screen for antibodies...and complement, and specialized cells such as macrophages and neutrophils that attack invading pathogens...presenting cells (APC) including dendritic cells and macrophages. Antigen presentation is mediated by the MHC ...function of innate immune cells including NK cells, macrophages, dendritic cells, and granulocytes. TLR The toll...system. They are found on dendritic cells and macrophages and sense conserved microbial molecules during...FRWS, MGC20661 CSF1 colony stimulating factor 1 (macrophage) MCSF, MGC31930 CSF1R colony stimulating factor...

  3. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    ...on using bacteriophages as bacterial therapeutics. This idea was incorporated into Novophage Therapeutics...engineering and scientific concepts to engineer bacteriophages and use those to detect unwanted bacteria. ...idea of starting a company around engineered bacteriophages. He decided to quit his "safety net" at the...

  4. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...phagocytosis (ADCP), Fcγ receptors on macrophages bind IgG antibodies. The macrophages then engulf and digest the...antibody-dependent cellular phagocytosis for effective macrophage-based cancer immunotherapy.Sci. Adv.8,eabl9171...

  5. Cre-lox system

    Type
    Collection
    ...Background The Cre-lox system, derived from P1 bacteriophage, is a potent and specific system for controlling... expressed at higher levels in mice than P1 bacteriophage Cre, facilitating high rates of Cre-driven recombination.../mCherry Cre and mCherry ProC3 AAV Roska 126700 pHAGE2-EF1aL-Cre-IRES-NeoR-W Cre EF-1 alpha Lentiviral...in Mammalian Cells by the Cre Recombinase of Bacteriophage P1. Proc Natl Acad Sci. Jul;85(14):5166-70. .... PubMed . Sternberg N, Hamilton D. 1981. Bacteriophage P1 Site-Specific Recombination. I. Recombination...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ... produces modified dendritic cells (DCs) and macrophages (M1 and M2) that help create welcoming environments...over-express CD47 to escape recognition by circulating macrophages. Tumor cells also downregulate the expression...

  7. CRISPR Plasmids - Base Edit

    Type
    Collection
    ... the DNA glycosylase inhibitor (UGI) or the bacteriophage Mu- derived Gam protein (Mu- GAM). While many...

  9. Synthetic Biology - Overview

    Type
    Collection
    ...Lipolytica Promoters Anderson Lab Plasmids and Phagemids Balazsi Lab Negative Autoregulation and Yeast-...

  16. Working with Nuclear Receptors

    Type
    Blog Post
    ...physiological states such as adipogenesis and macrophage activation. Our mass spectrometry-affinity purification...

  18. Hot Plasmids - October 2020

    Type
    Blog Post
    ... Cas protein identified exclusively in huge bacteriophage genomes. It’s active in vitro and in human and...
Showing: 61 - 80 of 89 results