...TF ORF barcode PCR for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X scRNA-seq...

...use protocols that require restriction enzymes or PCR. The app contains a double digest tool that is only...Lastly, the Tm calculator helps you set up an optimal PCR depending on the primer sequence and the NEB polymerase...

...blogger post about colony PCR, I signed up immediately. I had used colony PCR before and thought it was...

...better for PCR and surveyor. I used DNA polymerase Takara Ex Taq ™ DNA Polymerase for my genomic PCR, but couldn't...conditions with 5% DMSO or without 5% DMSO. My genomic PCR didn’t work, do you have any suggestions? The Zhang...the protocol, then uses around 2-4ul in each 50ul PCR reaction. The gDNA extracted in this way is usually...groups have successfully used Herc II or Kapa Hifi in PCR. Maybe this Takara enzyme is not very robust in this...

...conjugates used in assays like proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020...(2007) Detecting antigens by quantitative immuno-PCR. Nat Protoc 2:1918–1930. https://doi.org/10.1038/...

...plagued by a steady barrage of problems. Why didn’t my PCR work? Why didn’t my ligation work? Why are there ... being able to figure out what went wrong with a PCR is a pretty niche skill; outside of a lab, where ...

... once decided that to teach high school students PCR, they first had to understand hydrogen bonds and ...unlikely to be able to follow a YouTube video on PCR, while a senior lab scientist is likely to be quickly...

...perform PCR against components of the viral vector using the harvested viral solution as a PCR template...

...developed by detecting these chimeric DNA fragments by PCR or next-generation sequencing. Figure 1: Overview...reversed so that DNA fragments can be detected by PCR or deep sequencing. A drawback of the original...

... Weigel and Glazebrook, 2002. Tips: Do a colony PCR on your agrobacterium: Getting a transformant takes...remaining starter culture can be used for colony PCR and glycerol stocks. Dip/drip plants more than once...

...presence of pathogens in environmental samples via PCR. We hope these plasmids will help Dr. Duedu inspire...

... use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels of contamination...detection, Uphoff et. al. outline a do it yourself PCR-based approach complete with freely available positive...

...facility requires you to perform your own Big Dye PCR amplification reaction (as opposed to using the all...

...Reaction (PCR) by using a biotinylated primer. Biotin can also be enzymatically incorporated during PCR using...

...differentiation process. Moreover, a splinkerette-PCR assay may be used to map the specific genetic loci...and D. J. Adams, “A high-throughput splinkerette-PCR method for the isolation and sequencing of retroviral...

...degrade the double helix, fragmenting plasmid DNA, PCR product, or chromosome. One of the main causes of...

...procedure daily before the advent of techniques like PCR and DNA synthesis. Most molecular biologists bought...

... technique for use in Drosophila cells that uses PCR to generate both an expression cassette for the Cas9.... The plasmids in the following articles provide PCR templates for amplification of the tag (eg GFP, Flag...expresses Cas9, PITCh-gRNA, & FBL-specific gRNA pCRIS-PITChv2-FBL - PITCh donor vector for EGFP-2A-PuroR...

... be co-transfected as PCR amplicons using a protocol similar to SpCas9 sgRNA PCR (Ran et al., Nature Protocols...codon-optimized SpCas9; sgRNA can be co-transfected as PCR amplicons (Ran et al., Nature Protocols 2013). Individual...

...single-stranded DNA sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited...