...Double‐Readout Lu minescence‐based T wo‐ Hy brid) : Combines in vivo BRET with ex vivo luminescence-based co-immunoprecipitation...Luciferase Blog: Luminescent Imaging with Nano-lanterns Collection Highlights Empty Backbones Expression Constructs... DULIP ( DU al L uminescence-based C o- I mmunoprecipitation) plasmids : Luminescence-based protein-protein...for assays of transcriptional regulation and bioluminescent reporters. Plasmid...Luciferase is the enzyme responsible for the bioluminescence found in a diverse number of organisms, ranging...delivered intracellularly in order to measure luminescence. Gaussia luciferase is secreted and more stable...time course experiments possible, but its short luminescence half-life makes it too dim for many experimental...

...FRET More Fluorescent Protein Resources: Empty Backbones Biosensors Subcellular localization Optogenetics...biosensors targeting small molecules or specific genes are also available. Custom biosensors can also ... linker-Venus-6aa linker-Venus V5V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 5 amino acid linker V17V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 17 amino acid linker V32V Anisotropy/brightness standard consisting of two Venus fluorescent ...connected via a 32 amino acid linker VVV Anisotropy/brightness standard consisting of three Venus fluorescent...amino acid linker, respectively VVVV Anisotropy/brightness standard consisting of four Venus fluorescent...

...developmental biology. With its rapid development (embryogenesis is complete in ~3 days), short generation time...research model. With more than 70% of human disease genes having an ortholog in zebrafish, this species has...This includes plasmids for genome editing, empty backbones, fluorescent proteins, reporters, biosensors, ...selection of plasmids expressing Cas9 and empty gRNA backbones. A few examples of useful zebrafish CRISPR resources...Keith Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9...with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools for...cassettes encoding fluorescent proteins for tagging genes at endogenous genomic loci in zebrafish. NTR 2.0...

...Protocol for Genomic Deletions in Mammalian Cell Lines CasPEDIA : An encyclopedia of Class 2 CRISPR systems... and quantify the efficiency of the targeted mutagenesis The amplicon sequence expected after HDR can ...identify positively or negatively selected sgRNAs, genes, or pathways. CrispyCrunch CrispyCrunch is used ...CRISPR screens targeting a long or short list of genes provided by the user. Read more about it on the ...tool that identifies putative target sites for S. pyogenes Cas9, S. thermophilus Cas9, or Cpf from your input...Arabidopsis, C. elegans , sea squirt, cavefish, Chinese hamster, fruit fly, human, rice fish, mouse, silk...mouse, rat, chicken, frog, zebrafish, fly, worm, Japanese rice fish, maize, Arabidopsis, and rice. Proof-of-concept...

...Caenorhabditis elegans. Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit...development, and aging. Scientists can knock down genes by feeding worms bacteria expressing dsRNA or use...editing to easily engineer specific transgenic worm lines. The transparent body of the C. elegans hermaphrodite...worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre expression plasmids...Description Gene/Insert PI Plasmids Expressing Worm Genes/Inserts The table below lists plasmids that contain... include plasmids expressing Cas9, empty gRNA backbones, and constructs for endogenous tagging. Some examples...Jorgensen Lab. A collection of plasmids that streamlines the production of targeting vectors for tag insertion...

...pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited here with...from the hiPS cell lines gRNA sequences used in the creation of the hiPS cell lines The following plasmids...proteins of key cellular structures in human cell lines. Depositor...proteins of key cellular structures in human cell lines of their choice. For a full description of how to...plasmids can be used to create cell lines with endogenously-tagged cellular structures. Addgene ID Plasmid...HIST1H2BJ-mEGFP AICSDP-52 mEGFP Histone H2B type 1-J Histones 109119 CTNNB1-mEGFP AICSDP-47 mEGFP Beta-catenin...nuclear antigen (PCNA) DNA replication foci IPSC cell lines can be ordered from the Allen Institute for Cell...

...Over time, a loss of motor neurons leads to limb weakness, difficulty speaking, swallowing and breathing...mechanisms are still poorly understood but several genes and mapped loci seem to be associated with the disease...known genetic mutations in one of the following genes: SOD1, C9ORF72, TARDBP, FUS and OPTN. Huntington's... a mutation in one of three different inherited genes: Presenilin 1, Presenilin 2, and APP gene.The majority... are a risk factor for late-onset AD. Many more genes have been associated with Alzheimer's disease, through...now investigating the role that these additional genes may play in Alzheimer's disease. Research Tools ...type or mutant ORFs, gene fragments, and gRNAs for genes linked to neurodegenerative disorders. Find plasmids...

...Gene Switch: These constructs contain two genes of interest, Genes A and B. When Cre is absent, only Gene...the orientation and location of the loxP sites determines how the genetic material will be rearranged. ...have developed constructs to activate/inactivate genes when Cre is present. By expressing Cre at specific...regulation. Promoter-regulated Cre: The promoter region defines the areas in which Cre will be expressed. Cre may... Mammalian Jacks 68544 AAV-Cre-GFP Cre CMV AAV Nestler 68552 HRE:GFP-pGK:Cre Cre and hypoxia-responsive...orientation, and type of target sites that flox your genes of interest makes FLEx switch a powerful experimental...genOway press release for more information. Empty backbones to regulate your gene of interest: ID Plasmid ...

...Institute: Patient-specific lines (Link opens in a new window) RSRT iPSC lines and isogenic controls , patient-specific... Resource Center MECP2 Animal Models Human Cell Lines CRISPR Tools Plasmids Resources The (Link opens ...global transcriptional regulator of thousands of genes and studies have suggested roles in transcriptional...almost 46% of all Rett syndrome cases. Research Milestones 1992 - MECP2 is discovered by Adrian Bird's lab...new window) PMID: 21074045 2017 - the Bird lab determines a minimally functional MECP2 that rescues neurological...opens in a new window) Envigo. The following cell lines are available directly from the labs in which they..., please email [email protected] The following cell lines are available directly from the labs in which they...

...top Empty Backbones The table below highlights plasmids that can be used as empty backbones for your gene...CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources Background...different functional variants of Cas9 and empty gRNA backbones. A few examples of useful plant CRISPR resources...includes CRISPR/Cas nucleases, base editors, gRNA backbones, and promoters for expression in monocots and ...CRISPR/Cas9 constructs - Sylvestre Marillonnet and Johannes Stuttmann. Combined fluorescent seed selection...hierarchical assembly of plant multigene constructs - Johannes Stuttmann. MoChlo: Modular Cloning Chloroplast...highlights plasmids that feature fluorescent or luminescent proteins for expression in plants. You can search...

...repeat cycles of expansion. Chemokines Chemokines, or chemotactic cytokines, are small proteins that can...factor FLJ40431 Return to top Chemokines Chemokines, or chemotactic cytokines, are small proteins that can...sites of inflammation throughout the body. Cytokines Cytokines are molecules that enable cells of the immune...motif) receptor 1 CCXCR1, GPR5 Return to top Cytokines Cytokines are molecules that enable cells of the immune...Addgene’s immunology collection contains plasmids for genes involved in innate and adaptive immune signaling...response. Browse Plasmids for Immunology Research Genes have been grouped into tables based on the Gene ...plasmids for each gene. Please note that not all genes have plasmids available for request. Antigen Presentation...

...perspective, sometimes it’s best to delete genes from or introduce new genes and mutations to the bacterial genome...collection contains a variety of tools to help you harness bacterial genomes for your research. Plasmid ID...inducible expression of wild-type Cas9 from S. pyogenes for inducing double stranded breaks. For a more... Visualization Technique/Tag PI Purpose Empty Backbones with Fluorescent protein tags Various Plasmids... associated transcription factors, and reporter genes. pCS-PesaRlux 47640 PesaR 3OC6HSL (Quorum sensing...expression of catalytically inactive Cas9 ( S. pyogenes ) which can be combined with expression of a gRNA... Plasmids containing easily measurable reporter genes (eg. LacZ or Fluorescent Proteins ) under the control...

...performs a chemiluminescent reaction in the presence of several different substrates. The luminescence produced...Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into plasmids. Plasmids...region is a stable part of the antibody, which determines the isotype of the antibody. B cells, the immune...tears, saliva, mucus) where it protects mucosal membranes Dimer with a valency of 4 IgA1; IgA2 IgD Coexpressed... epitope, or polyclonal, meaning there are many clones that are specific to multiple epitopes on one antigen...Sodium azide, an antimicrobial agent, can block some amines needed for conjugation reactions and should be ... tubulin (sometimes referred to as housekeeping genes). This allows for relative comparison of expression...

...provides basic guidelines for working with various types of viruses; however, these guidelines may differ ...Plasmids | Guide Adenoviruses are commonly used as vaccines because of the strong immunogenic response they... to produce other molecules or to express other genes. Viruses used to direct these alternate functions...viruses) for safety through the removal of certain genes that are required for viral replication. Thus, as...that naturally occurring viruses do. Instead, the genes that are required for viral replication are usually...beginning virus work. For general virus biosafety guidelines, check out Addgene's Biosafety Resource Guide...

...top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as having...to these genes below. ID Plasmid Description Industry PI For more information on these genes, see the ... is involved in beating of airway cilia.( 7 ) Cytokines - SARS-CoV-2 infection induces a cytokine storm...storm response in the body. For a full list of cytokines and related plasmids see our Immunology Resource...Spike Receptor Binding Domain Site-Saturation Mutagenesis Libraries - Libraries of variant Spike protein...

... identified that responded to stimuli, such as hormones or metal ions; however, these systems were confounded...On System In 1995, Gossen et al. used random mutagenesis to identify which amino acid residues of tetR...interest rtTA or TetR expression plasmid Stable cell lines can be made that continuously express a system component...tetracycline-free. Tetracycline-inducible Empty Backbones Find a construct that will allow you to insert...promoter, and Blasticidin; can be used to make cell lines Tet-On Advanced; Plasmid 26730 has hygro resistance...-69. PubMed . Transcriptional activation by tetracyclines in mammalian cells. Gossen M, Freundlieb S, ...

...Imaging More Fluorescent Protein Resources: Empty Backbones FRET Subcellular localization Optogenetics Background...variants have been shown to have high effective brightness and allow for single or multicolor imaging. Photo-activatable...Vivo Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal...Crimson Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643/670 12.7 piRFP670-N1... Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690/717 (after photoactivation...

...will promptly notify you by email within a few business days of your request to let you know that we don...solution. You must reply to that email within 10 business days in order to proceed with any adjustments ...analysis, you will be notified by email. If Addgene determines that our current queue is too high and we do ... will notify you by email before, or within 10 business days of ordering. We will give you the option ...solution. You must reply to this email within 10 business days in order to proceed with any adjustments ...

... discriminate between positive and negative clones. Clones with insertion will show only linearized plasmid...FAQs Can you summarize HR template vector design guidelines? A few notes below are considerations for designing...plasmid of ~8.5 kb (only AgeI will be able to cut). Clones without insertion will show a ~1kb and ~7.5kb fragment...) on each colony in order to screen for positive ones. - Or - If your HR template has a selection marker... primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the reaction...

...recombinase-expressing mouse lines 2–9 and combinations of transgenic recombinase-expressing animal lines and retro-grade...these ORF fragments (which have become exons) determines the logical expression requirements of Cre and...Flp-recombinase-expressing transgenic mouse and rat lines. Plasmids In addition to EYFP and ChR2-EYFP, a large...genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene ID Plasmid Logic Sites and...opens in a new window) Mandelbaum G, Taranda J, Haynes TM, Hochbaum DR, Huang KW, Hyun M, Umadevi Venkataraju...