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  1. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose Glucose, methanol,...meaning it can utilize methanol as its sole source of carbon and energy (as well as glucose or glycerol). K....

  2. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these sequences...PMID: 31914802. Savy, A., Dickx, Y., Nauwynck, L., Bonnin, D., Merten, O.-W., & Galibert, L. (2017). Impact...

  3. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...Check Out Addgene's Empty Backbone Resource: Choosing Your Perfect Plasmid Backbone Read More: Plasmids...

  8. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...create an amplicon that  was cloned into a plasmid backbone for expression of the mAb heavy and light chain...modify the CH encoding sequence within the plasmid backbone to generate R-mAbs of different subclasses, including...

  9. Antibodies 101: ChIP

    Type
    Blog Post
    ...formaldehyde or UV light to create strong but reversible bonds. This will keep the protein:DNA complexes together...sample up and then processing with a homogenizer, but bone or especially fibrous tissues may need extra steps...

  10. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies on this plate...list of Bacterial Expression Systems Find Empty Backbones for Your Experiments    ...

  11. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...while expressing the gene. Briefly, the vector “backbone” in this system contains ccdB. A successful insertion...propagate and prep your ccdB-containing empty backbones but should never be used when selecting for recombinant...

  12. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ..., and as we all know, a structural component of bones and teeth. It is an enzyme co-factor, a crosslinking...the Cameleon-based FRET sensor D1ER, and GCaMPER. Bonus: There are also Cameleon-based FRET sensors available...

  13. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...that target the common features of viral vector backbones; once validated these universal primers can be... for functional assays depending on the vector backbone. When using qPCR based methods for viral titration...

  14. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...tagging the kinesins KIF5A and KIF5B as well as backbones for generating other SNAPf-, CLIPf-, and CLAP-...biosensor in optimal proportions from a vector backbone that allows lentiviral packaging as a single payload...

  15. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...Thankfully, this is a hot topic with many resources! In Bondeson et al, they review all the tags discussed here...informed choice! References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al....

  19. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... based on the copy number of the empty plasmid backbone or a researcher’s personal observations. If you..., buffering agents, and/or supplying additional carbon sources such as glycerol or glucose (in moderation...
Showing: 61 - 80 of 306 results